breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 119,184 T→G 11.8% F47V (TTT→GTT)  marB → multiple antibiotic resistance protein MarB
RA NZ_CP022686 119,210 C→G 7.3% G55G (GGC→GGG marB → multiple antibiotic resistance protein MarB
RA NZ_CP022686 196,840 A→C 7.4% N132T (AAC→ACC)  dtpA → dipeptide/tripeptide permease DtpA
RA NZ_CP022686 235,846 A→C 6.0% N287T (AAC→ACC)  pykF → pyruvate kinase PykF
RA NZ_CP022686 238,279 G→T 9.9% F100L (TTC→TTA sufE ← cysteine desulfuration protein SufE
RA NZ_CP022686 238,312 G→T 8.3% Y89* (TAC→TAA sufE ← cysteine desulfuration protein SufE
RA NZ_CP022686 238,317 G→T 13.1% L88I (CTT→ATT)  sufE ← cysteine desulfuration protein SufE
RA NZ_CP022686 238,323 A→C 20.8% F86V (TTT→GTT)  sufE ← cysteine desulfuration protein SufE
RA NZ_CP022686 263,831 A→C 7.7% intergenic (+38/+19) fadK → / ← ppsA medium‑chain fatty‑acid‑‑CoA ligase/phosphoenolpyruvate synthase
RA NZ_CP022686 298,027 A→C 11.3% F60C (TTT→TGT)  chbC ← PTS N,N'‑diacetylchitobiose transporter subunit IIC
RA NZ_CP022686 314,813 T→G 8.5% V204G (GTC→GGC)  ynjC → ABC transporter permease
RA NZ_CP022686 349,106 A→C 12.7% T607P (ACC→CCC)  yeaG → protein kinase YeaG
RA NZ_CP022686 360,246 T→G 11.9% E28D (GAA→GAC yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 360,256 C→A 25.6% G25V (GGA→GTA)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 377,274 A→C 11.0% K136N (AAA→AAC manX → PTS mannose transporter subunit IIAB
RA NZ_CP022686 418,379 T→G 6.9% G49G (GGT→GGG znuB → zinc ABC transporter permease subunit ZnuB
RA NZ_CP022686 442,342 A→C 8.9% S80A (TCG→GCG)  CIW80_RS02340 ← phage tail protein
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 474,664 C→A 8.2% E297* (GAA→TAA)  torZ ← trimethylamine N‑oxide reductase TorZ
RA NZ_CP022686 482,349 T→G 6.9% N172T (AAC→ACC)  flhA ← flagellar biosynthesis protein FlhA
RA NZ_CP022686 498,662 A→C 7.4% C183G (TGT→GGT)  araH ← arabinose ABC transporter permease AraH
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 601,295 T→G 17.7% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 20.8% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,337 T→G 8.8% V1621G (GTT→GGT)  irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 693,021 A→C 8.1% T121P (ACG→CCG)  CIW80_RS03550 → YaiO family outer membrane beta‑barrel protein
RA NZ_CP022686 694,391 C→A 11.6% intergenic (+151/‑45) CIW80_RS03555 → / → CIW80_RS03560 transposase/PfkB family carbohydrate kinase
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 759,039 A→T 14.7% L219* (TTA→TAA)  CIW80_RS03960 ← glycosyltransferase family 2 protein
RA NZ_CP022686 764,997 T→G 11.6% N41T (AAC→ACC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 765,001 A→T 8.8% Y40N (TAC→AAC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,452 G→T 24.3% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,467 C→A 16.9% intergenic (‑56/+171) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,472 G→T 9.4% intergenic (‑61/+166) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 837,561 G→T 10.1% intergenic (‑134/+89) thiM ← / ← rcnR hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR
RA NZ_CP022686 859,954 A→C 9.7% R605R (CGA→CGC yehM → DUF5682 family protein
RA NZ_CP022686 896,395 T→G 10.8% W17G (TGG→GGG)  yeiG → S‑formylglutathione hydrolase
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,025 G→T 11.8% intergenic (+94/‑163) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,049 A→T 12.2% intergenic (+118/‑139) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,080 G→T 14.3% intergenic (+149/‑108) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,090 C→A 14.3% intergenic (+159/‑98) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 1,005,082 T→A 9.5% Y379* (TAT→TAA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,040,909 A→C 8.3% E232D (GAA→GAC rbn → ribonuclease BN
RA NZ_CP022686 1,065,978 A→C 6.2% F37V (TTT→GTT)  yfbS ← SLC13 family permease
RA NZ_CP022686 1,100,944 T→G 11.0% D187A (GAC→GCC)  aroC ← chorismate synthase
RA NZ_CP022686 1,131,892 A→C 9.9% V119G (GTG→GGG)  dsdC ← DNA‑binding transcriptional regulator DsdC
RA NZ_CP022686 1,151,773 T→G 7.0% N379T (AAC→ACC)  alaC ← alanine transaminase
RA NZ_CP022686 1,167,039 A→C 11.2% F96V (TTC→GTC)  mntH ← Nramp family divalent metal transporter
RA NZ_CP022686 1,174,272 A→C 13.5% intergenic (+1/‑43) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,174,305 A→T 7.7% intergenic (+34/‑10) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,195,297 C→A 10.2% K132N (AAG→AAT cysP ← thiosulfate/sulfate ABC transporter substrate‑binding protein CysP
RA NZ_CP022686 1,209,908 T→G 21.0% D396A (GAC→GCC)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,209,917 G→T 14.5% P393Q (CCA→CAA)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,237,929 C→A 6.3% L299F (TTG→TTT tmcA ← tRNA cytosine(34) acetyltransferase TmcA
RA NZ_CP022686 1,239,833 G→T 12.3% intergenic (‑130/+38) ypfJ ← / ← purC neutral zinc metallopeptidase/phosphoribosylaminoimidazolesuccinocarboxamide synthase
RA NZ_CP022686 1,269,551 T→G 9.9% K1124N (AAA→AAC CIW80_RS06320 ← DUF823 domain‑containing adhesin
RA NZ_CP022686 1,278,783 T→G 7.5% D242A (GAC→GCC)  bamB ← outer membrane protein assembly factor BamB
RA NZ_CP022686 1,319,604 T→G 13.1% N327T (AAC→ACC)  glyA ← serine hydroxymethyltransferase
RA NZ_CP022686 1,349,193 A→C 6.6% K401N (AAA→AAC srmB → ATP‑dependent RNA helicase SrmB
RA NZ_CP022686 1,360,724 G→T 13.4% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,727 A→T 9.5% intergenic (‑62/+262) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,756 C→A 10.8% intergenic (‑91/+233) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,370,612 C→A 14.4% D81Y (GAT→TAT)  rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase RluD
RA NZ_CP022686 1,371,086 G→T 23.6% E34* (GAA→TAA)  bamD → outer membrane protein assembly factor BamD
RA NZ_CP022686 1,441,726 A→C 8.8% L612V (TTA→GTA)  hypF ← carbamoyltransferase HypF
RA NZ_CP022686 1,441,772 A→C 6.6% R596R (CGT→CGG hypF ← carbamoyltransferase HypF
RA NZ_CP022686 1,443,795 T→G 6.7% T149P (ACC→CCC)  hydN ← electron transport protein HydN
RA NZ_CP022686 1,447,222 A→C 10.0% A27A (GCT→GCG hycI ← hydrogenase maturation peptidase HycI
RA NZ_CP022686 1,460,615 A→C 10.9% K673N (AAA→AAC flhA → formate hydrogenlyase transcriptional activator FlhA
RA NZ_CP022686 1,479,607 A→C 11.5% intergenic (‑16/+177) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,479,633 A→C 12.8% intergenic (‑42/+151) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,250 T→G 15.4% V229V (GTT→GTG CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,258 G→T 14.4% S232I (AGT→ATT)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,289 T→G 8.6% R242R (CGT→CGG CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,310 G→T 13.6% M249I (ATG→ATT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,323 C→A 12.1% P254T (CCT→ACT)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,353 G→C 8.2% A264P (GCT→CCT) ‡ CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,355 T→A 9.4% A264A (GCT→GCA) ‡ CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,535,318 A→T 8.7% M20L (ATG→TTG)  fucI → L‑fucose isomerase
RA NZ_CP022686 1,535,365 A→C 24.2% K35N (AAA→AAC fucI → L‑fucose isomerase
RA NZ_CP022686 1,547,003 G→C 11.7% intergenic (+25/‑9) CIW80_RS07705 → / → CIW80_RS07710 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,558,395 A→C 15.4% K464N (AAA→AAC vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,597,235 T→G 11.7% N19H (AAC→CAC)  thyA ← thymidylate synthase
RA NZ_CP022686 1,604,862 A→C 7.5% F379V (TTC→GTC)  lplT ← lysophospholipid transporter LplT
RA NZ_CP022686 1,632,480 T→G 7.2% V159G (GTC→GGC)  arcC → carbamate kinase
RA NZ_CP022686 1,632,501 T→G 17.8% V166G (GTT→GGT)  arcC → carbamate kinase
RA NZ_CP022686 1,674,224 T→G 14.9% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 12.4% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 13.0% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,705,974 T→G 12.8% intergenic (‑584/‑71) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,708,105 T→G 6.4% A157A (GCT→GCG galP → galactose/proton symporter
RA NZ_CP022686 1,716,145 A→C 13.8% T42P (ACC→CCC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,716,154 A→C 8.3% T45P (ACC→CCC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,716,167 A→C 16.3% N49T (AAC→ACC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,716,202 A→C 7.6% T61P (ACC→CCC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,758,771 T→G 5.1% intergenic (+18/‑254) sat → / → CIW80_RS08700 serine protease autotransporter toxin Sat/transposase
RA NZ_CP022686 1,784,279 A→C 8.8% K111T (AAG→ACG)  CIW80_RS08870 → IS66 family transposase
RA NZ_CP022686 1,805,002 C→A 6.5% pseudogene (283/934 nt) CIW80_RS09020 → nucleotidyl transferase AbiEii/AbiGii toxin family protein
RA NZ_CP022686 1,805,056 A→C 9.7% pseudogene (337/934 nt) CIW80_RS09020 → nucleotidyl transferase AbiEii/AbiGii toxin family protein
RA NZ_CP022686 1,833,750 A→T 13.7% V466D (GTC→GAC)  gspD ← type II secretion system secretin GspD
RA NZ_CP022686 1,911,602 T→G 14.2% N224T (AAC→ACC)  CIW80_RS09520 ← MurR/RpiR family transcriptional regulator
RA NZ_CP022686 2,016,153 T→G 6.0% noncoding (121/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,016,170 A→C 7.7% noncoding (104/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,027,390 A→C 7.4% K80N (AAA→AAC agaE → PTS N‑acetylgalactosamine transporter subunit IID
RA NZ_CP022686 2,047,984 A→C 8.6% R624R (CGT→CGG deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,089 T→G 19.7% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,094 G→T 9.2% T254T (ACC→ACA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,126 C→A 5.3% D244Y (GAT→TAT)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,056,147 A→C 5.8% G621G (GGT→GGG infB ← translation initiation factor IF‑2
RA NZ_CP022686 2,061,804 A→C 10.6% K391N (AAA→AAC argG → argininosuccinate synthase
RA NZ_CP022686 2,110,457 T→G 10.5% K12N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,110,465 T→G 8.1% T10P (ACC→CCC)  rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,110,472 T→G 11.0% K7N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,130,829 A→C 7.8% V237G (GTT→GGT)  mreC ← rod shape‑determining protein MreC
RA NZ_CP022686 2,148,109 T→G 14.6% V124G (GTG→GGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,115 T→G 10.5% L126R (CTG→CGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,132 C→G 16.6% L132V (CTG→GTG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,184,330 G→T 11.4% F64L (TTC→TTA rplO ← 50S ribosomal protein L15
RA NZ_CP022686 2,185,075 A→C 7.2% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,188,229 G→T 5.4% intergenic (‑98/+67) rplN ← / ← rpsQ 50S ribosomal protein L14/30S ribosomal protein S17
RA NZ_CP022686 2,194,054 G→T 6.3% Q444K (CAA→AAA)  gspA ← type II secretion system protein GspA
RA NZ_CP022686 2,209,074 C→A 5.2% A258A (GCG→GCT chiA ← bifunctional chitinase/lysozyme
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,222,531 T→G 6.8% F417V (TTT→GTT)  yheS → ABC transporter ATP‑binding protein
RA NZ_CP022686 2,232,148 T→G 10.0% K48N (AAA→AAC ppiA ← peptidylprolyl isomerase A
RA NZ_CP022686 2,232,217 C→A 6.1% A25A (GCG→GCT ppiA ← peptidylprolyl isomerase A
RA NZ_CP022686 2,272,831 G→T 6.7% intergenic (+73/‑24) greB → / → yhgF transcription elongation factor GreB/Tex family protein
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,353,173 T→G 5.7% L108V (TTG→GTG)  yhhN → lysoplasmalogenase
RA NZ_CP022686 2,357,291 A→C 8.8% N26T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,357,322 T→A 10.7% S36R (AGT→AGA dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,357,324 A→C 14.7% N37T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,362,954 T→G 6.2% F152V (TTT→GTT)  nikB → nickel ABC transporter permease subunit NikB
RA NZ_CP022686 2,380,936 A→C 7.7% K186N (AAA→AAC pitA → inorganic phosphate transporter PitA
RA NZ_CP022686 2,386,496 T→G 5.6% K417N (AAA→AAC prlC ← oligopeptidase A
RA NZ_CP022686 2,393,013 T→G 5.1% intergenic (+151/‑2) slp → / → dctR outer membrane lipoprotein Slp/LuxR C‑terminal‑related transcriptional regulator
RA NZ_CP022686 2,394,287 T→G 18.8% N115T (AAC→ACC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,398,896 A→C 15.8% K206N (AAA→AAC hutW → heme anaerobic degradation radical SAM methyltransferase ChuW/HutW
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,460,576 C→A 10.2% M1M (ATG→ATT) † yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,460,581 A→C 22.7% intergenic (‑3/‑155) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
RA NZ_CP022686 2,468,070 G→T 10.3% G9V (GGA→GTA)  CIW80_RS26235 → hypothetical protein
RA NZ_CP022686 2,470,422 T→G 12.4% K72N (AAA→AAC glyS ← glycine‑‑tRNA ligase subunit beta
RA NZ_CP022686 2,516,216 T→C 7.7% S542S (TCT→TCC upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,516,426 T→A 7.7% G612G (GGT→GGA upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,516,872 A→C 6.1% K761T (AAA→ACA)  upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,533,236 A→C 7.3% L301R (CTG→CGG)  tdh ← L‑threonine 3‑dehydrogenase
RA NZ_CP022686 2,555,403 G→C 18.5% intergenic (‑30/+36) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH
RA NZ_CP022686 2,555,413 A→C 19.6% intergenic (‑40/+26) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH
RA NZ_CP022686 2,559,398 T→G 7.0% N461T (AAC→ACC)  ligB ← NAD‑dependent DNA ligase LigB
RA NZ_CP022686 2,572,705 A→C 15.8% G191G (GGT→GGG CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,574,429 T→G 14.6% N230T (AAC→ACC)  CIW80_RS12895 ← class II fructose‑bisphosphate aldolase family protein
RA NZ_CP022686 2,574,441 A→T 6.3% V226E (GTA→GAA)  CIW80_RS12895 ← class II fructose‑bisphosphate aldolase family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,632,661 G→T 6.0% F112L (TTC→TTA dgoT ← MFS transporter
RA NZ_CP022686 2,638,855 C→A 6.7% intergenic (‑170/+70) yidB ← / ← gyrB YidB family protein/DNA topoisomerase (ATP‑hydrolyzing) subunit B
RA NZ_CP022686 2,736,585 T→A 7.6% Y183N (TAC→AAC) ‡ rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,736,586 A→C 11.9% Y183S (TAC→TCC) ‡ rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,743,563 T→G 10.4% noncoding (65/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,743,601 G→T 9.2% intergenic (+16/‑27) CIW80_RS13750 → / → CIW80_RS13755 tRNA‑Leu/tRNA‑Pro
RA NZ_CP022686 2,780,149 T→G 6.5% V690G (GTC→GGC)  metE → 5‑methyltetrahydropteroyltriglutamate‑‑ homocysteine S‑methyltransferase
RA NZ_CP022686 2,801,864 T→G 5.2% L16F (TTA→TTC CIW80_RS14030 ← 2‑dehydro‑3‑deoxy‑6‑phosphogalactonate aldolase
RA NZ_CP022686 2,808,132 A→T 5.8% I443F (ATT→TTT)  ubiB → ubiquinone biosynthesis regulatory protein kinase UbiB
RA NZ_CP022686 2,814,142 A→C 5.5% L341R (CTG→CGG)  fadA ← acetyl‑CoA C‑acyltransferase FadA
RA NZ_CP022686 2,836,157 C→A 6.1% intergenic (+275/+107) polA → / ← yihA DNA polymerase I/ribosome biogenesis GTP‑binding protein YihA/YsxC
RA NZ_CP022686 2,868,740 T→G 22.0% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 13.1% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,871,422 T→G 8.5% N149T (AAC→ACC)  fdnG ← formate dehydrogenase‑N subunit alpha
RA NZ_CP022686 2,920,408 C→A 6.7% Q62K (CAA→AAA)  CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 2,920,418 C→A 5.6% A65E (GCA→GAA)  CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 2,981,625 A→C 9.2% D571A (GAC→GCC)  rpoC → DNA‑directed RNA polymerase subunit beta'
RA NZ_CP022686 2,989,627 T→G 7.5% Q39H (CAA→CAC thiC ← phosphomethylpyrimidine synthase ThiC
RA NZ_CP022686 3,020,229 T→G 11.0% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,070,986 T→G 8.4% G53G (GGT→GGG CIW80_RS15290 → 2‑oxoglutarate dehydrogenase E1 component
RA NZ_CP022686 3,090,093 G→T 7.2% R12S (CGC→AGC)  yjcB ← YjcB family protein
RA NZ_CP022686 3,136,940 T→G 8.6% D161A (GAC→GCC)  phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,150,252 T→G 13.1% F20V (TTT→GTT)  rdcB → YjcZ‑like family protein
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,200,595 A→C 7.0% V199G (GTT→GGT)  frdA ← fumarate reductase (quinol) flavoprotein subunit
RA NZ_CP022686 3,280,824 T→G 6.5% F15V (TTT→GTT)  yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,280,835 T→G 6.0% G18G (GGT→GGG yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,281,004 T→G 10.2% F75V (TTT→GTT)  yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,286,882 C→A 6.3% Q63K (CAA→AAA)  cybC → cytochrome b562
RA NZ_CP022686 3,334,291 C→A 7.3% G250C (GGT→TGT)  fecD ← Fe(3+) dicitrate ABC transporter permease subunit FecD
RA NZ_CP022686 3,348,342 A→T 8.3% intergenic (‑158/‑42) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,345 G→T 7.1% intergenic (‑161/‑39) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,357 T→G 6.7% intergenic (‑173/‑27) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,387,644 A→C 8.0% L222R (CTG→CGG)  sgcQ ← BtpA family protein SgcQ
RA NZ_CP022686 3,390,616 C→G 14.2% A143P (GCC→CCC)  sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,390,625 C→A 16.7% D140Y (GAT→TAT)  sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,704 T→G 15.6% T530P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,451,834 C→A 8.5% H275N (CAT→AAT)  lgoD → L‑galactonate oxidoreductase
RA NZ_CP022686 3,461,200 C→A 11.5% intergenic (‑9/+26) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,215 A→T 12.4% intergenic (‑24/+11) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,223 G→T 15.0% intergenic (‑32/+3) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,229 T→G 23.0% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,496,444 T→G 10.9% I270S (ATC→AGC)  thrA → bifunctional aspartate kinase/homoserine dehydrogenase I
RA NZ_CP022686 3,547,355 T→G 6.7% V99G (GTT→GGT)  folA → type 3 dihydrofolate reductase
RA NZ_CP022686 3,604,564 G→T 15.1% intergenic (+80/‑151) lpxC → / → secM UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM
RA NZ_CP022686 3,652,079 T→G 11.2% intergenic (‑88/+24) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,652,083 C→G 6.9% intergenic (‑92/+20) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,657,085 A→T 6.6% Y250N (TAC→AAC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,661,518 C→A 14.5% C9F (TGC→TTC)  pcnB ← polynucleotide adenylyltransferase PcnB
RA NZ_CP022686 3,734,367 T→G 19.5% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,770,508 T→G 6.4% intergenic (‑244/+219) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,770,509 A→T 9.6% intergenic (‑245/+218) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,770,526 C→G 7.9% intergenic (‑262/+201) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 14.6% L130V (TTA→GTA)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,798,092 C→A 8.9% F333L (TTC→TTA CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,806,265 T→A 9.3% Y1586N (TAC→AAC)  cdiA → contact‑dependent inhibition effector tRNA nuclease
RA NZ_CP022686 3,818,256 A→C 7.4% V311G (GTG→GGG)  pic ← serine protease autotransporter toxin Pic
RA NZ_CP022686 3,828,913 T→G 5.5% G131G (GGT→GGG CIW80_RS19170 → hypothetical protein
RA NZ_CP022686 3,868,846 T→G 9.1% T383P (ACG→CCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,881,917 C→A 5.2% S224* (TCG→TAG)  fdeC → inverse autotransporter adhesin FdeC
RA NZ_CP022686 3,885,499 A→C 12.5% intergenic (+2/+123) fdeC → / ← CIW80_RS19455 inverse autotransporter adhesin FdeC/helix‑turn‑helix domain‑containing protein
RA NZ_CP022686 3,909,251 G→T 6.1% D285Y (GAC→TAC)  pdeL → DNA‑binding transcriptional activator/c‑di‑GMP phosphodiesterase PdeL
RA NZ_CP022686 3,925,289 A→C 11.5% N156T (AAC→ACC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,928,345 T→G 13.7% F277V (TTC→GTC)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,928,355 C→G 11.9% A280G (GCT→GGT) ‡ prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,928,356 T→G 8.3% A280A (GCT→GCG) ‡ prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,928,361 A→T 7.2% D282V (GAT→GTT)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,928,369 T→G 8.7% W285G (TGG→GGG)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,935,324 T→G 11.7% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,940,707 A→C 9.2% F314V (TTT→GTT)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,949,969 T→G 11.1% D141A (GAC→GCC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 3,949,972 T→G 5.4% N140T (AAC→ACC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 3,977,611 A→C 6.7% E73D (GAA→GAC phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 3,981,933 T→G 12.4% intergenic (+70/‑86) proY → / → malZ proline‑specific permease ProY/maltodextrin glucosidase
RA NZ_CP022686 3,984,841 T→G 9.3% R67R (CGT→CGG queA → tRNA preQ1(34) S‑adenosylmethionine ribosyltransferase‑isomerase QueA
RA NZ_CP022686 4,014,242 A→T 13.1% intergenic (+88/‑256) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,250 C→G 11.8% intergenic (+96/‑248) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,032,292 A→C 15.2% E106D (GAA→GAC glnK → P‑II family nitrogen regulator
RA NZ_CP022686 4,056,962 C→A 19.6% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,056,978 G→T 9.1% E143* (GAA→TAA)  adk → adenylate kinase
RA NZ_CP022686 4,067,840 A→C 5.7% K7N (AAA→AAC ybaQ → HigA family addiction module antitoxin
RA NZ_CP022686 4,079,328 G→C 7.8% S170* (TCA→TGA)  ybbO ← NADP(+)‑dependent aldehyde reductase
RA NZ_CP022686 4,079,363 A→C 11.3% A158A (GCT→GCG ybbO ← NADP(+)‑dependent aldehyde reductase
RA NZ_CP022686 4,118,545 G→T 6.7% S252* (TCA→TAA)  nfrA ← bacteriophage adsorption protein NfrA
RA NZ_CP022686 4,188,944 A→C 8.0% V321G (GTA→GGA)  rlpA ← endolytic peptidoglycan transglycosylase RlpA
RA NZ_CP022686 4,213,261 A→T 6.6% M189L (ATG→TTG)  ubiF → 3‑demethoxyubiquinol 3‑hydroxylase
RA NZ_CP022686 4,214,103 T→A 11.0% intergenic (‑3/+35) CIW80_RS21080 ← / ← CIW80_RS21085 tRNA‑Gln/tRNA‑Gln
RA NZ_CP022686 4,214,435 G→C 9.4% intergenic (‑8/+27) CIW80_RS21095 ← / ← CIW80_RS21100 tRNA‑Gln/tRNA‑Gln
RA NZ_CP022686 4,287,456 T→G 10.3% noncoding (47/76 nt) CIW80_RS21475 → tRNA‑Val
RA NZ_CP022686 4,287,503 T→G 7.9% noncoding (16/76 nt) CIW80_RS21480 → tRNA‑Lys
RA NZ_CP022686 4,331,751 T→G 10.2% T352P (ACG→CCG)  ybhR ← ABC transporter permease
RA NZ_CP022686 4,368,341 T→G 9.4% D47A (GAC→GCC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,412,284 A→C 13.6% W241G (TGG→GGG)  ybjT ← SDR family oxidoreductase
RA NZ_CP022686 4,435,749 A→C 9.5% intergenic (‑66/+57) cydD ← / ← trxB cysteine/glutathione ABC transporter permease/ATP‑binding protein CydD/thioredoxin‑disulfide reductase
RA NZ_CP022686 4,440,324 C→A 14.1% P794Q (CCG→CAG)  ftsK → DNA translocase FtsK
RA NZ_CP022686 4,440,334 A→T 15.4% Q797H (CAA→CAT ftsK → DNA translocase FtsK
RA NZ_CP022686 4,445,543 C→G 10.1% intergenic (+49/‑190) serS → / → dmsA serine‑‑tRNA ligase/dimethylsulfoxide reductase subunit A
RA NZ_CP022686 4,562,550 T→G 15.8% N140H (AAC→CAC)  putA ← trifunctional transcriptional regulator/proline dehydrogenase/L‑glutamate gamma‑semialdehyde dehydrogenase
RA NZ_CP022686 4,571,354 C→A 10.3% E87D (GAG→GAT pgaC ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
RA NZ_CP022686 4,587,091 T→G 12.9% N609T (AAC→ACC)  CIW80_RS22915 ← enterotoxin
RA NZ_CP022686 4,587,098 A→T 6.2% Y607N (TAT→AAT)  CIW80_RS22915 ← enterotoxin
RA NZ_CP022686 4,589,367 T→G 7.5% T247P (ACC→CCC)  CIW80_RS22920 ← PatB family C‑S lyase
RA NZ_CP022686 4,632,182 T→G 11.3% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,678,033 T→G 38.6% intergenic (+31/‑90) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,678,047 A→C 26.0% intergenic (+45/‑76) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,764,877 T→G 12.4% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,880 T→G 5.9% intergenic (‑60/‑201) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,904 G→T 10.6% intergenic (‑84/‑177) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,780,176 A→C 12.1% intergenic (‑75/+95) phoP ← / ← purB two‑component system response regulator PhoP/adenylosuccinate lyase
RA NZ_CP022686 4,783,334 A→C 12.7% V32G (GTC→GGC)  mnmA ← tRNA 2‑thiouridine(34) synthase MnmA
RA NZ_CP022686 4,804,059 A→C 9.9% E620A (GAG→GCG)  CIW80_RS24225 → phage terminase large subunit family protein
RA NZ_CP022686 4,814,961 T→G 10.4% F61C (TTT→TGT)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,814,990 T→G 10.5% F71V (TTT→GTT)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,839,687 T→G 8.8% N144T (AAC→ACC)  minD ← septum site‑determining protein MinD
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,905,191 T→G 15.8% L106R (CTT→CGT)  CIW80_RS24815 → coenzyme F390 synthetase
RA NZ_CP022686 4,910,213 A→C 8.5% F48V (TTT→GTT)  purU ← formyltetrahydrofolate deformylase
RA NZ_CP022686 4,918,846 C→A 9.9% Q387H (CAG→CAT adhE ← bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
RA NZ_CP022686 4,919,017 T→G 8.2% E330D (GAA→GAC adhE ← bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
RA NZ_CP022686 4,932,144 T→G 9.2% F124V (TTT→GTT)  tonB → TonB system transport protein TonB
RA NZ_CP022686 4,952,020 T→G 6.6% V205G (GTC→GGC)  topA → type I DNA topoisomerase
RA NZ_CP022686 4,980,895 A→C 10.0% C101W (TGT→TGG sapD ← peptide ABC transporter ATP‑binding protein SapD
RA NZ_CP022686 5,001,157 G→T 13.4% D161Y (GAT→TAT)  ompG → monomeric porin OmpG
RA NZ_CP022686 5,043,648 G→C 5.3% V1093V (GTG→GTC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,855 T→A 10.9% G1162G (GGT→GGA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,182 T→A 6.1% G1271G (GGT→GGA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,614 T→C 8.1% G1415G (GGT→GGC CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMUT1‑hok‑empty 4,123:1 +T 94.8% intergenic (‑341/–) putative rom (RNA I inhibition modulator protein) ← /  –/–
RA pMUT1‑hok‑empty 4,127 C→A 100% intergenic (‑345/–) putative rom (RNA I inhibition modulator protein) ← /  –/–
RA pMUT1‑hok‑empty 4,132 4 bp→TA 100% intergenic (‑350/–) putative rom (RNA I inhibition modulator protein) ← /  –/–

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 400–2618 3048–2957 340–2649 8835 [8822] [8663] 9063 [JNG15_RS00005]–mobA [JNG15_RS00005],JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pMUT1‑hok‑empty 1256–1317 3987–1721 405–2732 7481 [7175] [6991] 8618 putative HTH domain protein–putative rom (RNA I inhibition modulator protein) putative HTH domain protein,uncharacterized protein,hypothetical protein,putative rom (RNA I inhibition modulator protein)

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 1522881 =NA (NA)8 (0.000) 8/250 NT NA intergenic (‑394/‑72) yqcC/CIW80_RS07590 YqcC family protein/IS3‑like element ISEc52 family transposase
?pMUT1‑hok‑empty 1135 = NA (NA)intergenic (–/+582) –/putative HTH domain protein –/–
* ? pMUT1‑hok‑empty 456 =5315 (0.450)7716 (0.660) 206/250 NT 67.5% intergenic (–/+1261) –/putative HTH domain protein –/–
?pMUT1‑hok‑empty 4136 = 2099 (0.180)intergenic (‑354/–) putative rom (RNA I inhibition modulator protein)/– –/–
* ? pMUT1‑hok‑empty = 4917850 (0.670)5825 (0.620) 168/200 NT 58.2% intergenic (–/+1226) –/putative HTH domain protein –/–
?pMUT1‑hok‑empty = 4098 2090 (0.220)intergenic (‑316/–) putative rom (RNA I inhibition modulator protein)/– –/–