breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 177,506 T→G 8.1% Q140H (CAA→CAC uidB ← glucuronide transporter
RA NZ_CP022686 298,027 A→C 11.8% F60C (TTT→TGT)  chbC ← PTS N,N'‑diacetylchitobiose transporter subunit IIC
RA NZ_CP022686 372,574 A→C 15.5% N275T (AAC→ACC)  sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 379,589 T→G 12.9% intergenic (+24/‑31) manZ → / → yobD PTS mannose transporter subunit IID/DUF986 family protein
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 496,077 T→A 8.6% N402Y (AAT→TAT)  otsA ← alpha,alpha‑trehalose‑phosphate synthase
RA NZ_CP022686 496,086 A→C 9.8% L399V (TTA→GTA)  otsA ← alpha,alpha‑trehalose‑phosphate synthase
RA NZ_CP022686 556,942 G→T 14.5% intergenic (+126/‑722) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 556,949 G→T 15.1% intergenic (+133/‑715) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 556,957 C→A 13.2% intergenic (+141/‑707) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 601,295 T→G 20.2% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 21.9% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 636,704 A→C 6.5% R455R (CGT→CGG clbM ← precolibactin export MATE transporter ClbM
RA NZ_CP022686 636,713 A→C 10.7% R452R (CGT→CGG clbM ← precolibactin export MATE transporter ClbM
RA NZ_CP022686 651,833 A→C 14.4% P243P (CCT→CCG clbJ ← colibactin non‑ribosomal peptide synthetase ClbJ
RA NZ_CP022686 694,438 G→T 16.3% M1M (ATG→ATT) † CIW80_RS03560 → PfkB family carbohydrate kinase
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 764,997 T→G 12.5% N41T (AAC→ACC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 765,008 A→T 16.8% I37I (ATT→ATA wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,452 G→T 12.4% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,467 C→A 19.1% intergenic (‑56/+171) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 881,733 A→C 17.9% intergenic (+248/+139) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 881,739 A→C 22.5% intergenic (+254/+133) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 881,742 A→C 22.3% intergenic (+257/+130) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 881,760 T→G 22.0% intergenic (+275/+112) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,046 G→T 14.5% intergenic (+115/‑142) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,049 A→T 12.2% intergenic (+118/‑139) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,051 G→T 14.2% intergenic (+120/‑137) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,080 G→T 16.8% intergenic (+149/‑108) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,090 C→A 15.0% intergenic (+159/‑98) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 957,112 T→G 11.8% T89P (ACC→CCC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 1,005,085 C→A 9.2% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,038,024 T→G 5.6% D409A (GAC→GCC)  menF ← isochorismate synthase MenF
RA NZ_CP022686 1,040,909 A→C 13.2% E232D (GAA→GAC rbn → ribonuclease BN
RA NZ_CP022686 1,137,229 A→C 6.0% R243R (CGT→CGG emrK ← multidrug efflux MFS transporter periplasmic adaptor subunit EmrK
RA NZ_CP022686 1,172,467 C→A 13.1% A130E (GCA→GAA)  yfeD → putative DNA‑binding transcriptional regulator
RA NZ_CP022686 1,174,272 A→C 16.2% intergenic (+1/‑43) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,197,146 C→G 11.8% L76V (CTG→GTG)  murQ → N‑acetylmuramic acid 6‑phosphate etherase
RA NZ_CP022686 1,209,935 A→C 15.2% V387G (GTG→GGG)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,213,456 A→C 29.2% L428R (CTG→CGG)  eutE ← aldehyde dehydrogenase EutE
RA NZ_CP022686 1,280,117 T→G 6.0% N8H (AAC→CAC)  yfgM ← ancillary SecYEG translocon subunit YfgM
RA NZ_CP022686 1,293,976 G→T 6.7% E212* (GAA→TAA)  sseA → 3‑mercaptopyruvate sulfurtransferase
RA NZ_CP022686 1,308,527 C→A 5.1% F28L (TTC→TTA yphA → DoxX family protein
RA NZ_CP022686 1,360,724 G→T 9.5% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,750 G→T 6.5% intergenic (‑85/+239) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,370,556 T→G 20.0% P99P (CCA→CCC rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase RluD
RA NZ_CP022686 1,418,017 A→C 5.3% T383P (ACC→CCC)  emrB → multidrug efflux MFS transporter permease subunit EmrB
RA NZ_CP022686 1,443,824 T→G 5.2% D139A (GAC→GCC)  hydN ← electron transport protein HydN
RA NZ_CP022686 1,447,198 T→A 7.4% V35V (GTA→GTT hycI ← hydrogenase maturation peptidase HycI
RA NZ_CP022686 1,464,955 G→T 6.7% E158* (GAA→TAA)  pphB → protein‑serine/threonine phosphatase
RA NZ_CP022686 1,479,607 A→C 10.0% intergenic (‑16/+177) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,479,610 G→C 6.5% intergenic (‑19/+174) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,310 G→T 12.5% M249I (ATG→ATT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,323 C→A 16.1% P254T (CCT→ACT) ‡ CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,324 C→A 17.9% P254H (CCT→CAT) ‡ CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,328 G→T 14.4% M255I (ATG→ATT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,361 G→T 23.1% V266V (GTG→GTT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,547,110 G→C 21.6% intergenic (+22/‑12) CIW80_RS07710 → / → CIW80_RS07715 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,558,325 T→G 6.3% L441R (CTG→CGG)  vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,558,395 A→C 13.2% K464N (AAA→AAC vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,564,569 A→C 13.7% K190N (AAA→AAC CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,564,573 T→G 12.2% W192G (TGG→GGG)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,564,589 A→C 11.1% N197T (AAC→ACC)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,632,501 T→G 9.7% V166G (GTT→GGT)  arcC → carbamate kinase
RA NZ_CP022686 1,632,508 C→A 12.4% I168I (ATC→ATA arcC → carbamate kinase
RA NZ_CP022686 1,633,846 T→G 29.2% N253T (AAC→ACC)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,640,925 A→C 16.1% R7S (AGA→AGC ygfM → molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,674,224 T→G 13.5% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 13.8% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 13.5% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,698,738 T→A 13.5% Q455L (CAG→CTG)  tkt ← transketolase
RA NZ_CP022686 1,706,032 G→T 12.5% intergenic (‑642/‑13) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,710,453 A→C 7.4% intergenic (+48/‑32) endA → / → rsmE deoxyribonuclease I/16S rRNA (uracil(1498)‑N(3))‑methyltransferase
RA NZ_CP022686 1,716,167 A→C 12.2% N49T (AAC→ACC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,796,779 A→C 9.2% T1039P (ACC→CCC)  agn43 → autotransporter adhesin Ag43
RA NZ_CP022686 1,828,113 A→C 7.1% P84P (CCT→CCG gspK ← type II secretion system minor pseudopilin GspK
RA NZ_CP022686 1,833,756 T→G 13.3% Q464P (CAG→CCG)  gspD ← type II secretion system secretin GspD
RA NZ_CP022686 1,860,077 G→T 15.4% G116G (GGC→GGA CIW80_RS09255 ← YtfJ family protein
RA NZ_CP022686 1,911,602 T→G 10.1% N224T (AAC→ACC)  CIW80_RS09520 ← MurR/RpiR family transcriptional regulator
RA NZ_CP022686 1,954,923 T→G 20.1% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 1,964,332 A→C 6.8% G27G (GGT→GGG mug ← G/U mismatch‑specific DNA glycosylase
RA NZ_CP022686 1,965,173 A→C 10.2% R86R (CGT→CGG nfeF ← NADPH‑dependent ferric chelate reductase
RA NZ_CP022686 2,016,175 T→G 5.5% noncoding (99/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,016,225 T→G 5.5% noncoding (49/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,049,094 G→T 9.5% T254T (ACC→ACA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,126 C→A 7.1% D244Y (GAT→TAT)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,056,168 A→C 7.5% G614G (GGT→GGG infB ← translation initiation factor IF‑2
RA NZ_CP022686 2,061,785 A→C 7.8% E385A (GAG→GCG)  argG → argininosuccinate synthase
RA NZ_CP022686 2,076,554 T→G 8.1% intergenic (+24/‑209) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,110,465 T→G 14.3% T10P (ACC→CCC)  rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,123,241 A→C 12.4% F208V (TTT→GTT)  tldD ← metalloprotease TldD
RA NZ_CP022686 2,148,115 T→G 15.0% L126R (CTG→CGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,122 T→G 9.1% S128R (AGT→AGG panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,141 T→G 10.8% F135V (TTC→GTC)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,172,177 A→C 7.6% G87G (GGT→GGG smg ← DUF494 family protein Smg
RA NZ_CP022686 2,179,877 C→G 9.2% K35N (AAG→AAC) ‡ rplQ ← 50S ribosomal protein L17
RA NZ_CP022686 2,179,878 T→G 9.5% K35T (AAG→ACG) ‡ rplQ ← 50S ribosomal protein L17
RA NZ_CP022686 2,179,916 G→T 8.5% R22R (CGC→CGA rplQ ← 50S ribosomal protein L17
RA NZ_CP022686 2,185,075 A→C 12.3% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,272,287 A→C 5.3% K2N (AAA→AAC greB → transcription elongation factor GreB
RA NZ_CP022686 2,305,978 T→A 8.1% S220C (AGT→TGT)  CIW80_RS11625 ← fimbrial biogenesis usher protein
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,357,324 A→C 18.3% N37T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,361,489 T→G 16.0% V188G (GTT→GGT)  nikA → nickel ABC transporter substrate‑binding protein
RA NZ_CP022686 2,394,287 T→G 17.1% N115T (AAC→ACC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,410,556 A→C 5.7% pseudogene (2792/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,460,522 T→A 10.2% V19V (GTA→GTT yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,460,581 A→C 20.6% intergenic (‑3/‑155) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
RA NZ_CP022686 2,516,873 A→C 7.5% K761N (AAA→AAC upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,521,024 T→G 7.0% V229G (GTT→GGT)  lldP → L‑lactate permease
RA NZ_CP022686 2,546,394 A→C 11.7% V95G (GTT→GGT)  waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,546,400 A→C 10.7% L93R (CTG→CGG)  waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,559,398 T→G 5.1% N461T (AAC→ACC)  ligB ← NAD‑dependent DNA ligase LigB
RA NZ_CP022686 2,572,705 A→C 12.8% G191G (GGT→GGG CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,572,715 G→T 9.6% S188Y (TCC→TAC)  CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,574,407 T→G 6.8% E237D (GAA→GAC CIW80_RS12895 ← class II fructose‑bisphosphate aldolase family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,632,825 G→T 9.3% Q58K (CAG→AAG)  dgoT ← MFS transporter
RA NZ_CP022686 2,701,400 G→T 6.0% R328S (CGC→AGC)  mdtD ← multidrug transporter subunit MdtD
RA NZ_CP022686 2,717,385 T→G 8.7% F96V (TTT→GTT)  ilvA → threonine ammonia‑lyase, biosynthetic
RA NZ_CP022686 2,736,573 G→C 8.4% E179Q (GAA→CAA)  rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,736,585 T→A 10.8% Y183N (TAC→AAC)  rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,743,601 G→T 16.7% intergenic (+16/‑27) CIW80_RS13750 → / → CIW80_RS13755 tRNA‑Leu/tRNA‑Pro
RA NZ_CP022686 2,780,110 T→G 11.6% F677C (TTT→TGT)  metE → 5‑methyltetrahydropteroyltriglutamate‑‑ homocysteine S‑methyltransferase
RA NZ_CP022686 2,801,864 T→G 7.9% L16F (TTA→TTC CIW80_RS14030 ← 2‑dehydro‑3‑deoxy‑6‑phosphogalactonate aldolase
RA NZ_CP022686 2,821,644 A→C 6.9% intergenic (+100/‑278) hemG → / → CIW80_RS14130 menaquinone‑dependent protoporphyrinogen IX dehydrogenase/16S ribosomal RNA
RA NZ_CP022686 2,868,740 T→G 19.2% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 16.0% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,871,422 T→G 7.6% N149T (AAC→ACC)  fdnG ← formate dehydrogenase‑N subunit alpha
RA NZ_CP022686 2,874,073 A→C 10.0% T62P (ACC→CCC)  CIW80_RS14390 → carbohydrate porin
RA NZ_CP022686 2,904,856 T→G 7.7% K237N (AAA→AAC ftsN ← cell division protein FtsN
RA NZ_CP022686 2,977,481 T→G 6.3% V558G (GTA→GGA)  rpoB → DNA‑directed RNA polymerase subunit beta
RA NZ_CP022686 2,989,627 T→G 8.1% Q39H (CAA→CAC thiC ← phosphomethylpyrimidine synthase ThiC
RA NZ_CP022686 3,013,752 T→G 6.9% V471G (GTG→GGG)  aceK → bifunctional isocitrate dehydrogenase kinase/phosphatase
RA NZ_CP022686 3,017,622 A→C 11.0% K839N (AAA→AAC metH → methionine synthase
RA NZ_CP022686 3,020,229 T→G 14.8% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,070,980 A→T 10.5% V51V (GTA→GTT CIW80_RS15290 → 2‑oxoglutarate dehydrogenase E1 component
RA NZ_CP022686 3,070,986 T→G 11.3% G53G (GGT→GGG CIW80_RS15290 → 2‑oxoglutarate dehydrogenase E1 component
RA NZ_CP022686 3,080,495 T→G 5.6% F170V (TTC→GTC)  CIW80_RS15320 → Ldh family oxidoreductase
RA NZ_CP022686 3,114,640 A→C 5.1% G40G (GGT→GGG CIW80_RS15500 ← ABC transporter ATP‑binding protein
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,200,552 A→C 9.1% G213G (GGT→GGG frdA ← fumarate reductase (quinol) flavoprotein subunit
RA NZ_CP022686 3,200,595 A→C 5.9% V199G (GTT→GGT)  frdA ← fumarate reductase (quinol) flavoprotein subunit
RA NZ_CP022686 3,209,121 T→G 7.8% T236P (ACC→CCC)  rsgA ← small ribosomal subunit biogenesis GTPase RsgA
RA NZ_CP022686 3,278,001 G→T 9.4% E281* (GAG→TAG) ‡ ytfQ → galactofuranose ABC transporter substrate‑binding protein YtfQ
RA NZ_CP022686 3,278,003 G→T 19.2% E281D (GAG→GAT) ‡ ytfQ → galactofuranose ABC transporter substrate‑binding protein YtfQ
RA NZ_CP022686 3,317,619 G→T 6.9% intergenic (+37/+82) lptG → / ← yjgR LPS export ABC transporter permease LptG/DUF853 domain‑containing protein
RA NZ_CP022686 3,348,345 G→T 6.9% intergenic (‑161/‑39) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,347 A→T 6.8% intergenic (‑163/‑37) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,371 T→G 7.6% intergenic (‑187/‑13) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,387,116 A→C 8.1% F86V (TTT→GTT)  sgcA ← SgcA family PTS sugar transporter subunit IIA
RA NZ_CP022686 3,387,616 G→T 6.3% I231I (ATC→ATA sgcQ ← BtpA family protein SgcQ
RA NZ_CP022686 3,390,605 T→G 19.0% K146N (AAA→AAC sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,390,629 C→A 10.3% W138C (TGG→TGT sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,704 T→G 11.1% T530P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,451,843 G→C 15.2% E278Q (GAA→CAA)  lgoD → L‑galactonate oxidoreductase
RA NZ_CP022686 3,461,229 T→G 14.5% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,461,231 C→G 10.2% noncoding (82/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,604,565 A→T 13.7% intergenic (+81/‑150) lpxC → / → secM UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM
RA NZ_CP022686 3,634,591 G→T 11.7% D260Y (GAT→TAT)  acnB → bifunctional aconitate hydratase 2/2‑methylisocitrate dehydratase
RA NZ_CP022686 3,634,608 G→T 5.2% P265P (CCG→CCT acnB → bifunctional aconitate hydratase 2/2‑methylisocitrate dehydratase
RA NZ_CP022686 3,634,614 A→C 9.6% K267N (AAA→AAC acnB → bifunctional aconitate hydratase 2/2‑methylisocitrate dehydratase
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,731,466 T→C 100% intergenic (+20/‑35) rrf → / → CIW80_RS18620 5S ribosomal RNA/tRNA‑Asp
RA NZ_CP022686 3,731,470 C→T 100% intergenic (+24/‑31) rrf → / → CIW80_RS18620 5S ribosomal RNA/tRNA‑Asp
RA NZ_CP022686 3,731,473 Δ1 bp 100% intergenic (+27/‑28) rrf → / → CIW80_RS18620 5S ribosomal RNA/tRNA‑Asp
RA NZ_CP022686 3,734,367 T→G 22.7% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,748,050 T→G 6.2% K22N (AAA→AAC CIW80_RS18735 ← hypothetical protein
RA NZ_CP022686 3,770,508 T→G 7.4% intergenic (‑244/+219) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,770,509 A→T 9.5% intergenic (‑245/+218) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,770,524 T→G 6.4% intergenic (‑260/+203) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 13.9% L130V (TTA→GTA)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,798,092 C→A 10.1% F333L (TTC→TTA CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,800,981 A→C 9.3% pseudogene (1257/1773 nt) CIW80_RS19080 → ShlB/FhaC/HecB family hemolysin secretion/activation protein
RA NZ_CP022686 3,806,266 A→C 9.3% Y1586S (TAC→TCC)  cdiA → contact‑dependent inhibition effector tRNA nuclease
RA NZ_CP022686 3,811,480 G→T 51.1% G122C (GGT→TGT)  CIW80_RS19090 → hypothetical protein
RA NZ_CP022686 3,850,157 A→T 8.0% intergenic (+55/‑37) gpt → / → frsA xanthine phosphoribosyltransferase/esterase FrsA
RA NZ_CP022686 3,868,846 T→G 12.5% T383P (ACG→CCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,895,534 T→G 7.1% intergenic (‑140/+44) CIW80_RS19510 ← / ← CIW80_RS19515 protein YkgH/autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 3,897,759 A→T 8.0% pseudogene (117/1083 nt) hyxB ← outer membrane autotransporter HyxB
RA NZ_CP022686 3,925,289 A→C 13.7% N156T (AAC→ACC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,950,011 T→G 7.7% H127P (CAC→CCC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 3,961,187 T→G 7.7% intergenic (+58/‑43) iraP → / → phoA anti‑adapter protein IraP/alkaline phosphatase
RA NZ_CP022686 4,004,135 T→G 8.6% R29R (CGT→CGG yajQ → nucleotide binding protein YajQ
RA NZ_CP022686 4,014,238 C→G 15.4% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,250 C→G 9.2% intergenic (+96/‑248) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,256 G→T 9.2% intergenic (+102/‑242) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,271 G→T 13.3% intergenic (+117/‑227) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,291 G→T 18.5% intergenic (+137/‑207) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,032,265 A→C 8.4% Q97H (CAA→CAC glnK → P‑II family nitrogen regulator
RA NZ_CP022686 4,056,962 C→A 18.3% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,056,975 G→T 12.0% V142L (GTA→TTA)  adk → adenylate kinase
RA NZ_CP022686 4,133,850 T→G 10.0% K113N (AAA→AAC ybdF ← MmcQ/YjbR family DNA‑binding protein
RA NZ_CP022686 4,196,276 T→G 17.2% E15D (GAA→GAC holA ← DNA polymerase III subunit delta
RA NZ_CP022686 4,331,751 T→G 8.3% T352P (ACG→CCG)  ybhR ← ABC transporter permease
RA NZ_CP022686 4,331,909 G→T 10.6% S299Y (TCC→TAC)  ybhR ← ABC transporter permease
RA NZ_CP022686 4,368,380 T→G 8.4% N34T (AAC→ACC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,440,297 T→A 11.3% V785D (GTT→GAT)  ftsK → DNA translocase FtsK
RA NZ_CP022686 4,535,285 A→C 9.5% K97T (AAG→ACG)  appA → bifunctional acid phosphatase/4‑phytase
RA NZ_CP022686 4,587,091 T→G 11.7% N609T (AAC→ACC)  CIW80_RS22915 ← enterotoxin
RA NZ_CP022686 4,632,182 T→G 11.0% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,764,877 T→G 15.5% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,880 T→G 10.8% intergenic (‑60/‑201) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,805,489 T→G 8.6% Y392D (TAT→GAT)  CIW80_RS24235 → phage portal protein
RA NZ_CP022686 4,809,441 T→G 10.9% G50G (GGT→GGG CIW80_RS24260 → head‑tail joining protein
RA NZ_CP022686 4,815,886 T→G 8.8% V25G (GTG→GGG)  CIW80_RS24305 → C40 family peptidase
RA NZ_CP022686 4,839,687 T→G 10.7% N144T (AAC→ACC)  minD ← septum site‑determining protein MinD
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,905,221 T→G 15.7% L116R (CTT→CGT)  CIW80_RS24815 → coenzyme F390 synthetase
RA NZ_CP022686 4,976,169 A→C 10.0% N826T (AAC→ACC)  CIW80_RS25180 → efflux RND transporter permease subunit
RA NZ_CP022686 5,042,304 T→G 16.6% N645K (AAT→AAG CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,558 T→C 5.5% S1063S (AGT→AGC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,855 T→A 9.3% G1162G (GGT→GGA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,359 C→G 5.2% V1330V (GTC→GTG CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMUT1‑hok‑empty 1,349 G→T 15.0% intergenic (–/+368)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,373 T→G 11.0% intergenic (–/+344)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,375 Δ1 bp 5.4% intergenic (–/+342)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,376 Δ1 bp 100% intergenic (–/+341)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,412 G→T 7.1% intergenic (–/+305)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,417 A→T 6.1% intergenic (–/+300)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,423 G→T 8.9% intergenic (–/+294)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,430 G→T 14.1% intergenic (–/+287)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 4,123:1 +T 93.8% intergenic (‑341/–) putative rom (RNA I inhibition modulator protein) ← /  –/–
RA pMUT1‑hok‑empty 4,127 C→A 100% intergenic (‑345/–) putative rom (RNA I inhibition modulator protein) ← /  –/–
RA pMUT1‑hok‑empty 4,132 4 bp→TA 100% intergenic (‑350/–) putative rom (RNA I inhibition modulator protein) ← /  –/–

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 388–2618 3052–2957 340–2665 7076 [7053] [7059] 7208 [JNG15_RS00005]–mobA [JNG15_RS00005],JNG15_RS00010,JNG15_RS00015,mobA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 3899666 =283 (1.300)26 (0.140) 19/218 NT 10.2% intergenic (‑40/+290) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 3899944 = 210 (1.110)intergenic (‑318/+12) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? NZ_CP022686 = 3899681267 (1.230)19 (0.100) 15/218 NT 7.9% intergenic (‑55/+275) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 = 3899927 210 (1.110)intergenic (‑301/+29) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? pMUT1‑hok‑empty 456 =3783 (0.450)5556 (0.660) 194/250 NT 67.6% intergenic (–/+1261) –/putative HTH domain protein –/–
?pMUT1‑hok‑empty 4136 = 1555 (0.180)intergenic (‑354/–) putative rom (RNA I inhibition modulator protein)/– –/–
* ? pMUT1‑hok‑empty = 4915654 (0.670)4283 (0.630) 152/200 NT 58.5% intergenic (–/+1226) –/putative HTH domain protein –/–
?pMUT1‑hok‑empty = 4098 1550 (0.230)intergenic (‑316/–) putative rom (RNA I inhibition modulator protein)/– –/–