breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 193,743 T→G 11.2% L309V (TTA→GTA)  rsxD → electron transport complex subunit RsxD
RA NZ_CP022686 340,516 A→C 12.3% F31V (TTT→GTT)  ydjK ← MFS transporter
RA NZ_CP022686 349,100 A→C 7.8% T605P (ACG→CCG)  yeaG → protein kinase YeaG
RA NZ_CP022686 349,106 A→C 13.5% T607P (ACC→CCC)  yeaG → protein kinase YeaG
RA NZ_CP022686 349,123 A→C 12.6% K612N (AAA→AAC yeaG → protein kinase YeaG
RA NZ_CP022686 350,045 G→C 13.4% K237N (AAG→AAC yeaH → YeaH/YhbH family protein
RA NZ_CP022686 360,227 T→G 17.3% T35P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 360,290 T→G 10.2% T14P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 372,556 T→G 10.5% L269R (CTG→CGG)  sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 372,574 A→C 13.3% N275T (AAC→ACC)  sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 512,703 T→G 8.8% intergenic (‑203/+27) sdiA ← / ← yecC transcriptional regulator SdiA/L‑cystine ABC transporter ATP‑binding protein YecC
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 596,637 T→G 10.1% G54G (GGT→GGG irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 597,830 A→C 10.6% D452A (GAC→GCC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,295 T→G 17.4% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 20.5% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 651,848 A→C 11.6% R238R (CGT→CGG clbJ ← colibactin non‑ribosomal peptide synthetase ClbJ
RA NZ_CP022686 693,021 A→C 8.1% T121P (ACG→CCG)  CIW80_RS03550 → YaiO family outer membrane beta‑barrel protein
RA NZ_CP022686 694,467 A→C 11.6% K11T (AAA→ACA)  CIW80_RS03560 → PfkB family carbohydrate kinase
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 764,997 T→G 10.9% N41T (AAC→ACC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 765,001 A→T 8.7% Y40N (TAC→AAC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 777,365 A→C 13.2% L216R (CTG→CGG)  gmd ← GDP‑mannose 4,6‑dehydratase
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,452 G→T 17.2% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 837,555 T→G 15.1% intergenic (‑128/+95) thiM ← / ← rcnR hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR
RA NZ_CP022686 878,883 T→G 8.9% intergenic (+75/+45) yohD → / ← yohF DedA family protein/SDR family oxidoreductase
RA NZ_CP022686 878,938 T→G 6.8% pseudogene (752/762 nt) yohF ← SDR family oxidoreductase
RA NZ_CP022686 881,733 A→C 19.9% intergenic (+248/+139) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 881,742 A→C 13.2% intergenic (+257/+130) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 881,760 T→G 15.6% intergenic (+275/+112) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 954,525 A→C 12.7% S38A (TCT→GCT)  ccmF ← cytochrome c‑type biogenesis heme lyase CcmF
RA NZ_CP022686 957,112 T→G 7.3% T89P (ACC→CCC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 1,005,085 C→A 11.4% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,034,493 T→G 9.6% T26P (ACC→CCC)  menC ← o‑succinylbenzoate synthase
RA NZ_CP022686 1,167,039 A→C 13.2% F96V (TTC→GTC)  mntH ← Nramp family divalent metal transporter
RA NZ_CP022686 1,174,416 A→T 8.5% intergenic (+26/‑21) CIW80_RS05860 → / → CIW80_RS05865 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,213,479 T→G 12.3% G420G (GGA→GGC eutE ← aldehyde dehydrogenase EutE
RA NZ_CP022686 1,280,117 T→G 6.4% N8H (AAC→CAC)  yfgM ← ancillary SecYEG translocon subunit YfgM
RA NZ_CP022686 1,349,193 A→C 10.5% K401N (AAA→AAC srmB → ATP‑dependent RNA helicase SrmB
RA NZ_CP022686 1,360,709 T→G 12.7% intergenic (‑44/+280) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,724 G→T 9.8% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,750 G→T 15.1% intergenic (‑85/+239) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,756 C→A 10.1% intergenic (‑91/+233) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,370,556 T→G 15.7% P99P (CCA→CCC rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase RluD
RA NZ_CP022686 1,479,607 A→C 11.5% intergenic (‑16/+177) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,479,625 G→C 9.3% intergenic (‑34/+159) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,258 G→T 11.9% S232I (AGT→ATT)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,361 G→T 27.8% V266V (GTG→GTT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,546,995 G→T 21.4% intergenic (+17/‑17) CIW80_RS07705 → / → CIW80_RS07710 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,558,395 A→C 13.0% K464N (AAA→AAC vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,619,287 C→A 7.5% intergenic (+76/+241) yqeG → / ← CIW80_RS07990 amino acid permease/tRNA‑Gly
RA NZ_CP022686 1,632,480 T→G 8.5% V159G (GTC→GGC)  arcC → carbamate kinase
RA NZ_CP022686 1,633,846 T→G 29.4% N253T (AAC→ACC)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,640,888 G→T 11.5% intergenic (+34/‑17) ssnA → / → ygfM putative aminohydrolase SsnA/molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,640,925 A→C 8.7% R7S (AGA→AGC ygfM → molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,674,244 T→G 16.4% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 10.5% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,697,914 T→G 7.5% intergenic (+23/+196) CIW80_RS08360 → / ← tkt prohibitin family protein/transketolase
RA NZ_CP022686 1,705,974 T→G 10.3% intergenic (‑584/‑71) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,716,145 A→C 11.4% T42P (ACC→CCC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,735,934 A→C 11.1% pseudogene (335/569 nt) papA ← P fimbria major subunit PapA
RA NZ_CP022686 1,828,110 A→C 8.1% R85R (CGT→CGG gspK ← type II secretion system minor pseudopilin GspK
RA NZ_CP022686 1,911,587 C→A 7.8% R229L (CGT→CTT)  CIW80_RS09520 ← MurR/RpiR family transcriptional regulator
RA NZ_CP022686 1,911,602 T→G 13.6% N224T (AAC→ACC)  CIW80_RS09520 ← MurR/RpiR family transcriptional regulator
RA NZ_CP022686 1,925,332 A→C 12.1% N151T (AAC→ACC)  ygiB → DUF1190 family protein
RA NZ_CP022686 1,954,923 T→G 12.7% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 1,954,932 G→T 7.8% Q89K (CAA→AAA)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 2,047,987 A→C 9.3% R623R (CGT→CGG deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,089 T→G 19.8% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,094 G→T 14.6% T254T (ACC→ACA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,056,168 A→C 10.5% G614G (GGT→GGG infB ← translation initiation factor IF‑2
RA NZ_CP022686 2,123,241 A→C 6.7% F208V (TTT→GTT)  tldD ← metalloprotease TldD
RA NZ_CP022686 2,130,829 A→C 13.0% V237G (GTT→GGT)  mreC ← rod shape‑determining protein MreC
RA NZ_CP022686 2,148,136 T→G 8.7% V133G (GTT→GGT)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,141 T→G 11.8% F135V (TTC→GTC)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,184,330 G→T 12.7% F64L (TTC→TTA rplO ← 50S ribosomal protein L15
RA NZ_CP022686 2,185,075 A→C 9.6% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,221,356 A→C 7.4% N25T (AAC→ACC)  yheS → ABC transporter ATP‑binding protein
RA NZ_CP022686 2,226,139 A→C 8.3% D9A (GAC→GCC)  crp → cAMP‑activated global transcriptional regulator CRP
RA NZ_CP022686 2,305,971 G→T 12.1% S222* (TCA→TAA)  CIW80_RS11625 ← fimbrial biogenesis usher protein
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,357,291 A→C 10.6% N26T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,361,489 T→G 9.2% V188G (GTT→GGT)  nikA → nickel ABC transporter substrate‑binding protein
RA NZ_CP022686 2,380,936 A→C 12.8% K186N (AAA→AAC pitA → inorganic phosphate transporter PitA
RA NZ_CP022686 2,386,496 T→G 8.2% K417N (AAA→AAC prlC ← oligopeptidase A
RA NZ_CP022686 2,394,287 T→G 20.0% N115T (AAC→ACC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,460,576 C→A 10.2% M1M (ATG→ATT) † yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,470,422 T→G 10.3% K72N (AAA→AAC glyS ← glycine‑‑tRNA ligase subunit beta
RA NZ_CP022686 2,516,426 T→A 10.5% G612G (GGT→GGA upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,516,867 T→A 12.4% N759K (AAT→AAA upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,516,873 A→C 8.4% K761N (AAA→AAC upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,546,394 A→C 18.3% V95G (GTT→GGT)  waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,546,400 A→C 15.1% L93R (CTG→CGG)  waaG ← glycosyltransferase family 4 protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,632,838 A→C 8.8% A53A (GCT→GCG dgoT ← MFS transporter
RA NZ_CP022686 2,643,712 A→C 6.6% L413R (CTG→CGG)  dnaA ← chromosomal replication initiator protein DnaA
RA NZ_CP022686 2,717,385 T→G 7.4% F96V (TTT→GTT)  ilvA → threonine ammonia‑lyase, biosynthetic
RA NZ_CP022686 2,719,166 A→T 7.7% I122I (ATT→ATA ilvY ← HTH‑type transcriptional activator IlvY
RA NZ_CP022686 2,736,585 T→A 8.4% Y183N (TAC→AAC)  rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,743,563 T→G 7.5% noncoding (65/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,743,585 A→C 22.8% noncoding (87/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,758,153 A→C 9.0% T49P (ACG→CCG)  yifL → lipoprotein
RA NZ_CP022686 2,834,886 A→C 9.0% K597N (AAA→AAC polA → DNA polymerase I
RA NZ_CP022686 2,868,740 T→G 21.8% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 14.7% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,871,422 T→G 12.4% N149T (AAC→ACC)  fdnG ← formate dehydrogenase‑N subunit alpha
RA NZ_CP022686 2,871,449 T→G 7.5% D140A (GAC→GCC)  fdnG ← formate dehydrogenase‑N subunit alpha
RA NZ_CP022686 2,904,852 T→G 7.7% T239P (ACG→CCG)  ftsN ← cell division protein FtsN
RA NZ_CP022686 2,904,856 T→G 8.8% K237N (AAA→AAC ftsN ← cell division protein FtsN
RA NZ_CP022686 2,907,549 T→G 9.4% T497P (ACG→CCG)  priA ← primosomal protein N'
RA NZ_CP022686 2,981,625 A→C 7.1% D571A (GAC→GCC)  rpoC → DNA‑directed RNA polymerase subunit beta'
RA NZ_CP022686 2,989,627 T→G 12.1% Q39H (CAA→CAC thiC ← phosphomethylpyrimidine synthase ThiC
RA NZ_CP022686 3,045,224 T→G 5.3% F279V (TTT→GTT)  lamB → maltoporin LamB
RA NZ_CP022686 3,090,101 A→C 9.2% V9G (GTT→GGT)  yjcB ← YjcB family protein
RA NZ_CP022686 3,124,925 A→C 6.6% F215V (TTT→GTT)  mdtN ← multidrug efflux transporter periplasmic adaptor subunit MdtN
RA NZ_CP022686 3,150,252 T→G 10.6% F20V (TTT→GTT)  rdcB → YjcZ‑like family protein
RA NZ_CP022686 3,184,651 A→C 10.0% intergenic (‑98/+18) cutA ← / ← dcuA divalent cation tolerance protein CutA/anaerobic C4‑dicarboxylate transporter DcuA
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,200,595 A→C 7.9% V199G (GTT→GGT)  frdA ← fumarate reductase (quinol) flavoprotein subunit
RA NZ_CP022686 3,252,190 T→G 13.2% K221N (AAA→AAC CIW80_RS16225 ← enoyl‑CoA hydratase/isomerase family protein
RA NZ_CP022686 3,281,062 T→G 12.0% V94G (GTG→GGG)  yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,348,371 T→G 6.5% intergenic (‑187/‑13) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,704 T→G 15.3% T530P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,461,127 A→C 16.0% noncoding (65/87 nt) CIW80_RS17335 ← tRNA‑Leu
RA NZ_CP022686 3,461,223 G→T 9.4% intergenic (‑32/+3) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,229 T→G 15.8% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,469,923 T→G 7.6% E122D (GAA→GAC yjjW ← YjjW family glycine radical enzyme activase
RA NZ_CP022686 3,604,565 A→T 10.8% intergenic (+81/‑150) lpxC → / → secM UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM
RA NZ_CP022686 3,652,079 T→G 11.4% intergenic (‑88/+24) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,734,367 T→G 17.5% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,770,508 T→G 13.1% intergenic (‑244/+219) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 13.6% L130V (TTA→GTA)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,806,265 T→A 11.3% Y1586N (TAC→AAC)  cdiA → contact‑dependent inhibition effector tRNA nuclease
RA NZ_CP022686 3,852,459 T→G 8.9% N178T (AAC→ACC)  phoE ← phosphoporin PhoE
RA NZ_CP022686 3,860,076 A→G 6.3% Y491H (TAC→CAC)  vat ← vacuolating autotransporter toxin Vat
RA NZ_CP022686 3,910,255 G→T 6.4% F69L (TTC→TTA yahB ← LysR substrate‑binding domain‑containing protein
RA NZ_CP022686 3,924,665 A→C 6.4% intergenic (+2/‑158) prpB → / → prpC methylisocitrate lyase/2‑methylcitrate synthase
RA NZ_CP022686 3,925,289 A→C 17.3% N156T (AAC→ACC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,925,304 A→C 11.0% Q161P (CAG→CCG)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,928,369 T→G 9.7% W285G (TGG→GGG)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,932,437 T→G 14.4% N186H (AAC→CAC)  lacA ← galactoside O‑acetyltransferase
RA NZ_CP022686 3,935,324 T→G 13.4% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,981,933 T→G 15.4% intergenic (+70/‑86) proY → / → malZ proline‑specific permease ProY/maltodextrin glucosidase
RA NZ_CP022686 4,014,238 C→G 13.4% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,018,211 T→G 6.1% intergenic (+146/‑42) clpX → / → lon ATP‑dependent protease ATP‑binding subunit ClpX/endopeptidase La
RA NZ_CP022686 4,056,962 C→A 20.4% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,072,082 T→G 10.0% G54G (GGT→GGG ybaT → amino acid permease
RA NZ_CP022686 4,074,902 G→T 10.4% S23* (TCG→TAG)  CIW80_RS20380 ← hypothetical protein
RA NZ_CP022686 4,078,994 C→A 8.9% intergenic (‑28/+33) cnoX ← / ← ybbO chaperedoxin/NADP(+)‑dependent aldehyde reductase
RA NZ_CP022686 4,133,839 T→G 12.2% K117T (AAA→ACA)  ybdF ← MmcQ/YjbR family DNA‑binding protein
RA NZ_CP022686 4,196,276 T→G 12.6% E15D (GAA→GAC holA ← DNA polymerase III subunit delta
RA NZ_CP022686 4,286,455 T→G 7.2% V38G (GTC→GGC)  cpoB → cell division protein CpoB
RA NZ_CP022686 4,287,491 T→G 6.9% noncoding (4/76 nt) CIW80_RS21480 → tRNA‑Lys
RA NZ_CP022686 4,331,929 C→A 12.2% M292I (ATG→ATT ybhR ← ABC transporter permease
RA NZ_CP022686 4,368,341 T→G 13.2% D47A (GAC→GCC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,368,372 T→G 15.1% S37R (AGC→CGC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,378,330 A→C 9.9% G128G (GGA→GGC pdeI → EAL domain‑containing protein
RA NZ_CP022686 4,387,288 A→C 9.1% F139V (TTT→GTT)  deoR ← DNA‑binding transcriptional repressor DeoR
RA NZ_CP022686 4,440,314 T→G 10.5% Y791D (TAT→GAT)  ftsK → DNA translocase FtsK
RA NZ_CP022686 4,440,334 A→T 15.6% Q797H (CAA→CAT ftsK → DNA translocase FtsK
RA NZ_CP022686 4,535,349 A→C 11.2% T118T (ACA→ACC appA → bifunctional acid phosphatase/4‑phytase
RA NZ_CP022686 4,571,419 C→G 7.5% A66P (GCA→CCA)  pgaC ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
RA NZ_CP022686 4,571,454 A→C 12.7% V54G (GTC→GGC)  pgaC ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
RA NZ_CP022686 4,587,091 T→G 14.6% N609T (AAC→ACC)  CIW80_RS22915 ← enterotoxin
RA NZ_CP022686 4,589,367 T→G 8.4% T247P (ACC→CCC)  CIW80_RS22920 ← PatB family C‑S lyase
RA NZ_CP022686 4,632,182 T→G 11.8% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,638,424 G→T 8.2% L109L (CTG→CTT CIW80_RS23235 → S/F1C fimbrial major subunit operon transcriptional regulator
RA NZ_CP022686 4,638,436 C→A 5.8% intergenic (+9/‑363) CIW80_RS23235 → / → focA S/F1C fimbrial major subunit operon transcriptional regulator/F1C fimbrial major subunit FocA
RA NZ_CP022686 4,642,951 A→C 7.9% N732T (AAC→ACC)  sfaF → S/F1C fimbrial usher SfaF/FocD
RA NZ_CP022686 4,677,993 G→C 5.3% V1037V (GTG→GTC agn43 → autotransporter adhesin Ag43
RA NZ_CP022686 4,678,041 A→C 15.5% intergenic (+39/‑82) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,762,516 T→G 10.1% K368N (AAA→AAC mfd ← transcription‑repair coupling factor
RA NZ_CP022686 4,780,176 A→C 20.3% intergenic (‑75/+95) phoP ← / ← purB two‑component system response regulator PhoP/adenylosuccinate lyase
RA NZ_CP022686 4,789,732 C→A 7.6% M57I (ATG→ATT CIW80_RS24095 ← DUF1828 domain‑containing protein
RA NZ_CP022686 4,804,059 A→C 11.4% E620A (GAG→GCG)  CIW80_RS24225 → phage terminase large subunit family protein
RA NZ_CP022686 4,809,441 T→G 12.7% G50G (GGT→GGG CIW80_RS24260 → head‑tail joining protein
RA NZ_CP022686 4,814,961 T→G 10.4% F61C (TTT→TGT)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,814,990 T→G 10.3% F71V (TTT→GTT)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,839,668 T→G 17.1% V150V (GTA→GTC minD ← septum site‑determining protein MinD
RA NZ_CP022686 4,839,687 T→G 10.4% N144T (AAC→ACC)  minD ← septum site‑determining protein MinD
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,918,873 T→G 10.4% Q378H (CAA→CAC adhE ← bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
RA NZ_CP022686 4,919,017 T→G 10.4% E330D (GAA→GAC adhE ← bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
RA NZ_CP022686 4,951,538 C→A 19.6% A44A (GCC→GCA topA → type I DNA topoisomerase
RA NZ_CP022686 5,041,188 C→T 6.5% S273S (AGC→AGT CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,041,191 G→A 9.0% A274A (GCG→GCA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,042,289 T→C 8.9% N640N (AAT→AAC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,042,304 T→G 12.1% N645K (AAT→AAG CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,347 A→G 5.4% N993S (AAT→AGT)  CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,855 T→A 10.0% G1162G (GGT→GGA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,942 C→G 5.0% V1191V (GTC→GTG CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,182 T→A 7.0% G1271G (GGT→GGA CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMUT1‑hok‑empty 4,123:1 +T 93.6% intergenic (‑341/–) putative rom (RNA I inhibition modulator protein) ← /  –/–
RA pMUT1‑hok‑empty 4,127 C→A 100% intergenic (‑345/–) putative rom (RNA I inhibition modulator protein) ← /  –/–
RA pMUT1‑hok‑empty 4,132 4 bp→TA 100% intergenic (‑350/–) putative rom (RNA I inhibition modulator protein) ← /  –/–

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 386–2618 3054–2957 340–2669 5507 [5491] [5498] 5542 [JNG15_RS00005]–mobA [JNG15_RS00005],JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pMUT1‑hok‑empty 1261–1367 3987–1722 356–2727 4037 [3885] [3756] 4181 putative HTH domain protein–putative rom (RNA I inhibition modulator protein) putative HTH domain protein,uncharacterized protein,hypothetical protein,putative rom (RNA I inhibition modulator protein)

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 3743172 =251 (1.270)20 (0.100) 14/246 NT 7.6% intergenic (+1/+253) CIW80_RS18695/CIW80_RS18700 IS66 family transposase/IS110‑like element ISEc45 family transposase
?NZ_CP022686 = 3744618 239 (1.220)intergenic (‑60/‑4) CIW80_RS18700/CIW80_RS18705 IS110‑like element ISEc45 family transposase/transposase
* ? pMUT1‑hok‑empty 456 =2927 (0.460)4134 (0.650) 216/248 NT 67.0% intergenic (–/+1261) –/putative HTH domain protein –/–
?pMUT1‑hok‑empty 4136 = 1149 (0.180)intergenic (‑354/–) putative rom (RNA I inhibition modulator protein)/– –/–
* ? pMUT1‑hok‑empty = 4914247 (0.670)3168 (0.620) 170/198 NT 58.3% intergenic (–/+1226) –/putative HTH domain protein –/–
?pMUT1‑hok‑empty = 4098 1145 (0.220)intergenic (‑316/–) putative rom (RNA I inhibition modulator protein)/– –/–