breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 182,618 A→C 9.3% F78V (TTT→GTT)  malI ← mal regulon transcriptional regulator MalI
RA NZ_CP022686 182,646 A→C 10.9% G68G (GGT→GGG malI ← mal regulon transcriptional regulator MalI
RA NZ_CP022686 202,265 A→C 9.8% W339G (TGG→GGG)  anmK ← anhydro‑N‑acetylmuramic acid kinase
RA NZ_CP022686 238,323 A→C 19.4% F86V (TTT→GTT)  sufE ← cysteine desulfuration protein SufE
RA NZ_CP022686 298,027 A→C 8.8% F60C (TTT→TGT)  chbC ← PTS N,N'‑diacetylchitobiose transporter subunit IIC
RA NZ_CP022686 418,374 T→G 11.9% F48V (TTT→GTT)  znuB → zinc ABC transporter permease subunit ZnuB
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 498,662 A→C 9.0% C183G (TGT→GGT)  araH ← arabinose ABC transporter permease AraH
RA NZ_CP022686 531,408 A→C 13.1% K44N (AAA→AAC fliF → flagellar basal‑body MS‑ring/collar protein FliF
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 601,295 T→G 21.5% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 21.1% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 684,585 A→C 15.3% intergenic (‑946/‑234) CIW80_RS26355 ← / → CIW80_RS03520 IS4 family transposase/TonB‑dependent receptor
RA NZ_CP022686 687,661 A→C 7.2% G107G (GGT→GGG CIW80_RS03525 ← ParB N‑terminal domain‑containing protein
RA NZ_CP022686 694,391 C→A 10.5% intergenic (+151/‑45) CIW80_RS03555 → / → CIW80_RS03560 transposase/PfkB family carbohydrate kinase
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 764,997 T→G 13.4% N41T (AAC→ACC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,441 C→A 26.4% intergenic (‑30/+197) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,452 G→T 18.8% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 864,173 T→G 17.5% intergenic (+39/+2) yehR → / ← yehS YehR family lipoprotein/DUF1456 family protein
RA NZ_CP022686 881,742 A→C 17.6% intergenic (+257/+130) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,090 C→A 15.2% intergenic (+159/‑98) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 1,005,073 A→C 9.4% E376D (GAA→GAC nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,005,085 C→A 16.6% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,064,076 A→C 6.2% T161P (ACG→CCG)  yfbR → 5'‑deoxynucleotidase
RA NZ_CP022686 1,100,944 T→G 14.8% D187A (GAC→GCC)  aroC ← chorismate synthase
RA NZ_CP022686 1,239,837 T→G 12.5% intergenic (‑134/+34) ypfJ ← / ← purC neutral zinc metallopeptidase/phosphoribosylaminoimidazolesuccinocarboxamide synthase
RA NZ_CP022686 1,280,117 T→G 9.1% N8H (AAC→CAC)  yfgM ← ancillary SecYEG translocon subunit YfgM
RA NZ_CP022686 1,325,398 C→A 14.0% E260* (GAA→TAA)  qseE ← two component system sensor histidine kinase QseE/GlrK
RA NZ_CP022686 1,360,724 G→T 15.0% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,750 G→T 9.8% intergenic (‑85/+239) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,441,800 T→G 6.4% N587T (AAC→ACC)  hypF ← carbamoyltransferase HypF
RA NZ_CP022686 1,447,199 A→C 11.6% V35G (GTA→GGA)  hycI ← hydrogenase maturation peptidase HycI
RA NZ_CP022686 1,447,222 A→C 10.1% A27A (GCT→GCG hycI ← hydrogenase maturation peptidase HycI
RA NZ_CP022686 1,452,244 A→C 10.0% G419G (GGT→GGG hycC ← formate hydrogenlyase subunit 3
RA NZ_CP022686 1,460,615 A→C 12.7% K673N (AAA→AAC flhA → formate hydrogenlyase transcriptional activator FlhA
RA NZ_CP022686 1,479,607 A→C 8.8% intergenic (‑16/+177) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,289 T→G 12.6% R242R (CGT→CGG CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,299 G→T 13.0% V246F (GTT→TTT)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,310 G→T 13.0% M249I (ATG→ATT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,355 T→A 13.2% A264A (GCT→GCA CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,547,110 G→C 13.9% intergenic (+22/‑12) CIW80_RS07710 → / → CIW80_RS07715 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,558,395 A→C 11.6% K464N (AAA→AAC vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,564,589 A→C 8.5% N197T (AAC→ACC)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,640,888 G→T 11.4% intergenic (+34/‑17) ssnA → / → ygfM putative aminohydrolase SsnA/molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,674,224 T→G 13.1% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,716,167 A→C 14.5% N49T (AAC→ACC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,775,845 T→G 9.5% N198T (AAC→ACC)  CIW80_RS08815 ← N‑acetylneuraminate epimerase
RA NZ_CP022686 1,833,759 A→T 16.6% V463E (GTA→GAA)  gspD ← type II secretion system secretin GspD
RA NZ_CP022686 1,833,799 T→G 12.5% T450P (ACG→CCG)  gspD ← type II secretion system secretin GspD
RA NZ_CP022686 1,911,580 G→T 13.9% S231R (AGC→AGA CIW80_RS09520 ← MurR/RpiR family transcriptional regulator
RA NZ_CP022686 2,056,168 A→C 7.8% G614G (GGT→GGG infB ← translation initiation factor IF‑2
RA NZ_CP022686 2,061,804 A→C 9.4% K391N (AAA→AAC argG → argininosuccinate synthase
RA NZ_CP022686 2,110,461 A→T 9.3% V11E (GTA→GAA)  rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,110,465 T→G 12.3% T10P (ACC→CCC)  rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,148,115 T→G 16.0% L126R (CTG→CGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,122 T→G 10.9% S128R (AGT→AGG panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,132 C→G 15.7% L132V (CTG→GTG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,136 T→G 9.9% V133G (GTT→GGT)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,159,673 C→A 17.1% A167E (GCA→GAA)  CIW80_RS10780 → amino acid ABC transporter substrate‑binding protein
RA NZ_CP022686 2,172,177 A→C 9.8% G87G (GGT→GGG smg ← DUF494 family protein Smg
RA NZ_CP022686 2,179,878 T→G 9.9% K35T (AAG→ACG)  rplQ ← 50S ribosomal protein L17
RA NZ_CP022686 2,184,330 G→T 17.8% F64L (TTC→TTA rplO ← 50S ribosomal protein L15
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,226,139 A→C 8.3% D9A (GAC→GCC)  crp → cAMP‑activated global transcriptional regulator CRP
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,340,264 A→C 8.3% V91G (GTC→GGC)  livF ← high‑affinity branched‑chain amino acid ABC transporter ATP‑binding protein LivF
RA NZ_CP022686 2,361,494 C→A 10.5% Q190K (CAG→AAG)  nikA → nickel ABC transporter substrate‑binding protein
RA NZ_CP022686 2,394,287 T→G 20.3% N115T (AAC→ACC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,394,323 A→T 9.3% L103H (CTC→CAC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,433,335 A→C 8.3% S1057A (TCG→GCG)  bcsC ← cellulose synthase complex outer membrane protein BcsC
RA NZ_CP022686 2,516,426 T→A 11.2% G612G (GGT→GGA upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,521,024 T→G 9.3% V229G (GTT→GGT)  lldP → L‑lactate permease
RA NZ_CP022686 2,572,705 A→C 12.8% G191G (GGT→GGG CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,572,721 A→C 9.0% F186C (TTT→TGT)  CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,574,429 T→G 9.3% N230T (AAC→ACC)  CIW80_RS12895 ← class II fructose‑bisphosphate aldolase family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,638,855 C→A 8.4% intergenic (‑170/+70) yidB ← / ← gyrB YidB family protein/DNA topoisomerase (ATP‑hydrolyzing) subunit B
RA NZ_CP022686 2,643,657 A→C 11.8% G431G (GGT→GGG dnaA ← chromosomal replication initiator protein DnaA
RA NZ_CP022686 2,717,385 T→G 10.5% F96V (TTT→GTT)  ilvA → threonine ammonia‑lyase, biosynthetic
RA NZ_CP022686 2,743,563 T→G 9.7% noncoding (65/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,868,740 T→G 25.2% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 15.6% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,904,852 T→G 8.6% T239P (ACG→CCG)  ftsN ← cell division protein FtsN
RA NZ_CP022686 2,920,428 A→C 7.0% K68N (AAA→AAC CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 3,020,229 T→G 13.2% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,080,461 T→G 10.2% R158R (CGT→CGG CIW80_RS15320 → Ldh family oxidoreductase
RA NZ_CP022686 3,114,653 A→C 7.3% V36G (GTC→GGC)  CIW80_RS15500 ← ABC transporter ATP‑binding protein
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,200,552 A→C 8.6% G213G (GGT→GGG frdA ← fumarate reductase (quinol) flavoprotein subunit
RA NZ_CP022686 3,252,190 T→G 13.2% K221N (AAA→AAC CIW80_RS16225 ← enoyl‑CoA hydratase/isomerase family protein
RA NZ_CP022686 3,348,334 T→G 10.8% intergenic (‑150/‑50) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,461,229 T→G 23.3% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,461,231 C→G 7.6% noncoding (82/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,520,304 A→C 7.1% N355T (AAC→ACC)  ileS → isoleucine‑‑tRNA ligase
MC JC NZ_CP022686 3,559,837 Δ2,343 bp 100% coding (10‑2352/2352 nt) polB ← DNA polymerase II
RA NZ_CP022686 3,604,564 G→T 14.2% intergenic (+80/‑151) lpxC → / → secM UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,661,518 C→A 10.3% C9F (TGC→TTC)  pcnB ← polynucleotide adenylyltransferase PcnB
RA NZ_CP022686 3,770,524 T→G 8.9% intergenic (‑260/+203) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,776,865 C→A 9.7% L123M (CTG→ATG)  CIW80_RS18945 → inovirus‑type Gp2 protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
MC JC NZ_CP022686 3,844,426 Δ1,036 bp 100% coding (2‑1037/1056 nt) dinB → DNA polymerase IV
RA NZ_CP022686 3,868,846 T→G 12.1% T383P (ACG→CCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,928,369 T→G 13.5% W285G (TGG→GGG)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,940,697 A→C 8.5% V317G (GTG→GGG)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,981,933 T→G 10.8% intergenic (+70/‑86) proY → / → malZ proline‑specific permease ProY/maltodextrin glucosidase
RA NZ_CP022686 4,014,242 A→T 15.1% intergenic (+88/‑256) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,056,962 C→A 17.1% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,072,068 T→G 9.9% F50V (TTT→GTT)  ybaT → amino acid permease
RA NZ_CP022686 4,074,874 A→C 6.7% R32R (CGT→CGG CIW80_RS20380 ← hypothetical protein
RA NZ_CP022686 4,194,942 T→G 7.0% T116P (ACC→CCC)  nadD ← nicotinate‑nucleotide adenylyltransferase
RA NZ_CP022686 4,196,276 T→G 14.4% E15D (GAA→GAC holA ← DNA polymerase III subunit delta
RA NZ_CP022686 4,287,456 T→G 10.2% noncoding (47/76 nt) CIW80_RS21475 → tRNA‑Val
RA NZ_CP022686 4,290,599 T→G 11.6% T121P (ACC→CCC)  ybgS ← YbgS‑like family protein
RA NZ_CP022686 4,331,940 A→C 14.7% F289V (TTT→GTT)  ybhR ← ABC transporter permease
RA NZ_CP022686 4,335,504 T→G 7.0% K58N (AAA→AAC ybhF ← ATP‑binding cassette domain‑containing protein
RA NZ_CP022686 4,485,614 T→G 12.3% F139V (TTT→GTT)  gloC → hydroxyacylglutathione hydrolase GloC
RA NZ_CP022686 4,573,936 C→G 8.5% E708Q (GAA→CAA)  pgaA ← poly‑beta‑1,6 N‑acetyl‑D‑glucosamine export porin PgaA
RA NZ_CP022686 4,632,182 T→G 9.6% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,642,951 A→C 9.0% N732T (AAC→ACC)  sfaF → S/F1C fimbrial usher SfaF/FocD
RA NZ_CP022686 4,662,707 T→G 8.1% pseudogene (750/1364 nt) CIW80_RS23370 ← IS4 family transposase
RA NZ_CP022686 4,678,041 A→C 19.2% intergenic (+39/‑82) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,678,047 A→C 34.4% intergenic (+45/‑76) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,712,763 A→C 24.5% A52A (GCT→GCG yceB ← lipoprotein
RA NZ_CP022686 4,764,904 G→T 11.6% intergenic (‑84/‑177) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,780,176 A→C 16.5% intergenic (‑75/+95) phoP ← / ← purB two‑component system response regulator PhoP/adenylosuccinate lyase
RA NZ_CP022686 4,789,705 A→T 6.0% L66L (CTT→CTA CIW80_RS24095 ← DUF1828 domain‑containing protein
MC JC NZ_CP022686 4,844,868 Δ1,662 bp 100% [umuD][umuC] [umuD], [umuC]
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,910,213 A→C 7.7% F48V (TTT→GTT)  purU ← formyltetrahydrofolate deformylase
RA NZ_CP022686 5,000,015 T→G 8.1% F156V (TTT→GTT)  CIW80_RS25310 → ABC transporter ATP‑binding protein
RA NZ_CP022686 5,041,191 G→A 8.0% A274A (GCG→GCA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,042,234 A→C 10.4% N622T (AAC→ACC)  CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,042,250 A→T 8.5% V627V (GTA→GTT CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,648 G→C 8.2% V1093V (GTG→GTC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,227 A→T 12.7% T1286T (ACA→ACT CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,614 T→C 6.8% G1415G (GGT→GGC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,641 C→T 7.4% N1424N (AAC→AAT CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMut‑Ompa‑D5‑IL2wt‑His6 2,031 G→A 100% intergenic (‑235/‑1500) putative rom (RNA I inhibition modulator protein) ← / → ompA –/–
RA pMut‑Ompa‑D5‑IL2wt‑His6 2,815 A→C 5.0% intergenic (‑1019/‑716) putative rom (RNA I inhibition modulator protein) ← / → ompA –/–
JC pMut‑Ompa‑D5‑IL2wt‑His6 3,595 Δ15 bp 100% [IL2wt no signal] [IL2wt no signal]

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 188–676 3086–2957 2282–2899 8342 [8085] [7903] 8470 JNG15_RS00005–mobA JNG15_RS00005,JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pMut‑Ompa‑D5‑IL2wt‑His6 4746–4859 5478–5074 216–733 3725 [3551] [0] NA [putative HTH domain protein] [putative HTH domain protein]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 262466 =2 (0.010)177 (1.060) 73/248 NT 98.9% coding (320/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1524130 NA (NA)intergenic (+8/+232) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
* ? NZ_CP022686 = 2624682 (0.010)177 (1.060) 68/248 NT 98.9% coding (322/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1761697 NA (NA)intergenic (‑72/‑70) CIW80_RS08710/CIW80_RS08720 IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase