breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 547,694 A→G pseudogene (114/1259 nt) ylbE → DUF1116 domain‑containing protein
RA 547,835 +G pseudogene (255/1259 nt) ylbE → DUF1116 domain‑containing protein
RA 556,858 A→T L36Q (CTG→CAG)  folD ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD
MC JC 654,214 Δ1,199 bp Y75_RS03225[dcuC] Y75_RS03225, [dcuC]
RA 987,574 G→T intergenic (‑170/+433) ompF ← / ← asnS porin OmpF/asparagine‑‑tRNA ligase
RA 1,093,686 T→C V130A (GTA→GCA)  dgcT → GGDEF domain‑containing protein
RA 1,197,797 C→T H366H (CAC→CAT icd → NADP‑dependent isocitrate dehydrogenase
RA 1,197,809 C→T T370T (ACC→ACT icd → NADP‑dependent isocitrate dehydrogenase
RA 1,197,822 T→C L375M (TTA→CTG)  icd → NADP‑dependent isocitrate dehydrogenase
RA 1,197,824 A→G L375M (TTA→CTG icd → NADP‑dependent isocitrate dehydrogenase
MC JC 1,301,041 Δ1,336 bp Y75_RS06530Y75_RS06530 Y75_RS06530, Y75_RS06530
RA 1,519,222 G→T V40V (GTG→GTT ortT → orphan toxin OrtT
RA 1,669,601 Δ1 bp coding (678/1221 nt) mlc ← DNA‑binding transcriptional repressor Mlc
RA 1,979,957 G→T intergenic (‑46/+398) flhD ← / ← Y75_RS09980 flagellar transcriptional regulator FlhD/IS5‑like element IS5 family transposase
RA 2,005,401 C→T E115K (GAA→AAA)  fliC ← H48 family flagellin FliC
MC JC 2,176,983 Δ1,199 bp Y75_RS10980[gatA] Y75_RS10980, [gatA]
RA 2,245,455 A→G S283P (TCG→CCG)  yeiB ← DUF418 domain‑containing protein YeiB
MC JC 2,320,795 Δ1,336 bp Y75_RS11650[rcsC] Y75_RS11650, Y75_RS11650, [rcsC]
RA 2,866,111 A→G pseudogene (97/993 nt) rpoS ← RNA polymerase sigma factor RpoS
RA 3,268,825 A→G T186A (ACA→GCA)  yhaC → YhaC family protein
MC JC 3,742,936 Δ1,336 bp Y75_RS18560Y75_RS18560 Y75_RS18560, Y75_RS18560
MC JC 3,746,911 Δ1,199 bp Y75_RS18585[tnaB] Y75_RS18585, [tnaB]
MC JC 3,750,591 Δ1,199 bp Y75_RS18600 Y75_RS18600
MC JC 3,958,123 Δ34 bp coding (1156‑1189/1287 nt) dctA → C4‑dicarboxylate transporter DctC
RA 4,005,879 T→C intergenic (‑305/‑78) yhiM ← / → Y75_RS19820 DUF2776 domain‑containing protein/YhiJ/YhiL family protein
MC JC 4,310,363 Δ1,199 bp Y75_RS21310[alsK] Y75_RS21310, [alsK]
RA 4,371,274 (A)5→3 pseudogene (179‑180/1304 nt) dcuA ← anaerobic C4‑dicarboxylate transporter DcuA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_007779 1197850 1213051 15202 64 [61] [55] 66 [icd]–[Y75_RS23470] [icd],ymfD,Y75_RS05950,lit,intE,xisE,Y75_RS24820,Y75_RS23460,Y75_RS05970,ymfK,ymfT,ymfL,Y75_RS23465,Y75_RS25370,Y75_RS05995,Y75_RS06000,Y75_RS06005,Y75_RS06010,ymfQ,Y75_RS06020,tfaP,tfaE,Y75_RS06035,pinE,mcrA,[Y75_RS23470]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_007779 380484 =NA (NA)150 (0.920) 80/466 0.3 NA coding (28/444 nt) Y75_RS01845 transferase
?NC_007779 = 381407 NA (NA)coding (518/914 nt) Y75_RS01860 IS3‑like element IS2 family transposase
* ? NC_007779 381401 =NA (NA)195 (1.210) 96/462 0.1 NA coding (512/914 nt) Y75_RS01860 IS3‑like element IS2 family transposase
?NC_007779 = 381812 NA (NA)pseudogene (264/264 nt) Y75_RS23175 acetyltransferase
* ? NC_007779 2519723 =NA (NA)117 (0.730) 62/460 0.8 93.7% intergenic (+33/‑54) nupC/Y75_RS12570 nucleoside permease NupC/IS4‑like element IS421 family transposase
?NC_007779 = 2600448 8 (0.050)coding (592/618 nt) hyfA hydrogenase 4 subunit HyfA
* ? NC_007779 = 2521059NA (NA)132 (0.830) 70/458 0.5 95.7% intergenic (+170/+30) Y75_RS12570/pdeA IS4‑like element IS421 family transposase/sensor domain‑containing phosphodiesterase
?NC_007779 2600437 = 6 (0.040)coding (581/618 nt) hyfA hydrogenase 4 subunit HyfA
* ? NC_007779 = 3186081NA (NA)180 (1.110) 90/464 0.1 98.9% coding (26/2466 nt) Y75_RS15850 fimbrial biogenesis outer membrane usher protein
?NC_007779 3512753 = 2 (0.010)coding (529/1026 nt) cytR DNA‑binding transcriptional regulator CytR
* ? NC_007779 = 32611330 (0.000)228 (1.430) 74/458 0.4 100% intergenic (‑59/‑69) tdcE/Y75_RS16205 2‑ketobutyrate formate‑lyase/pyruvate formate‑lyase/IS5‑like element IS5 family transposase
?NC_007779 3262333 = 0 (0.000)pseudogene (1183/1185 nt) tdcD propionate kinase
* ? NC_007779 = 35127591 (0.010)169 (1.040) 84/464 0.2 99.4% coding (535/1026 nt) cytR DNA‑binding transcriptional regulator CytR
?NC_007779 = 3744262 NA (NA)intergenic (‑90/+31) Y75_RS18560/yidZ IS3‑like element IS2 family transposase/HTH‑type transcriptional regulator YidZ
* ? NC_007779 = 3744263NA (NA)129 (0.790) 69/466 0.5 100% intergenic (‑91/+30) Y75_RS18560/yidZ IS3‑like element IS2 family transposase/HTH‑type transcriptional regulator YidZ
?NC_007779 3903274 = 0 (0.000)coding (37/825 nt) bax protein bax
* ? NC_007779 = 39032792 (0.010)174 (1.070) 81/464 0.2 98.9% coding (42/825 nt) bax protein bax
?NC_007779 = 4504190 NA (NA)intergenic (+10/+83) Y75_RS22280/Y75_RS22285 IS3‑like element IS2 family transposase/phosphoethanolamine transferase
* ? NC_007779 4310363 =NA (NA)95 (0.580) 46/466 1.8 100% intergenic (‑287/‑68) yjcS/Y75_RS21310 alkyl sulfatase YjcS/IS5‑like element IS5 family transposase
?NC_007779 = 4540919 0 (0.000)intergenic (+208/+60) yjhR/Y75_RS25450 helicase YjhR/HNH endonuclease
* ? NC_007779 = 4311556NA (NA)229 (1.410) 80/464 0.3 98.7% intergenic (+145/+1) Y75_RS21310/alsK IS5‑like element IS5 family transposase/allose kinase
?NC_007779 4540915 = 3 (0.020)intergenic (+204/+64) yjhR/Y75_RS25450 helicase YjhR/HNH endonuclease