New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	4385920 =	319 (1.090)	3 (0.010)	3/256	NT	0.98%	coding (39/1338 nt)	amiB	N‑acetylmuramoyl‑l‑alanine amidase II
	?	CP009273	= 4385916	308 (1.140)					coding (35/1338 nt)	amiB	N‑acetylmuramoyl‑l‑alanine amidase II
Rejected: Frequency below/above cutoff threshold.

TTTGTTTGATATCGAGCGCCACGGTGCGTTTGCTTTGATGGCTAAACGCATAATCAGGATCGCCAATAAAACTCAACGTTATCCGCGCCTGTTGATTACCGTTAGAAACCTGAATATCAGAGAGCGTCGCGGCACCCACCGGCGTGCACAGCAGCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP009273/4386077‑4385920 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cagcagcagcGTCGCTACCAACCAATTTCTGATGCGATACATCATCCCGCCACCTTTCAAAGGTTAACCGGCTAAACGCGCCAGCAACAATTCACCCGCAGAGGAAACCGCACTCACGCGCGCCTCACGGCCTTGTGCCTGATAATCAATGTGTATT  <  CP009273/4385916‑4385770                                                                                                                                                                                                                                                                                                                   TTTGTTTGATATCGAGCGCCACGGTGCGTTAGCTATGATGGCTAAACGCATACTCAGGATCGCCAATAACACTCAACGTTATCCGCGCCTGTTGATTACCGTTAGAAACCTGAATCTCAGAGAGCGTCGCGGCACCCACCGGCGTGCAC                                                                                                                                                              <  1:3865498/149‑1  TTGTTTGATATCGAGCGCCACGGTGCGTTTGCTTTGATGGCTAAACGCATAATCAGGATCGCCAATAAAACTCAACGTTATCCGCGCCTGTTGATTACCGTTAGAAACCTGAATATCAGAGAGCGTCGCGGCACCCACCGGCGTGCACA                                                                                                                                                             <  2:2749135/149‑1   TGTTTGATATCGAGCGCCACGGTGCGTTTGCTTTGATGGCTAAACGCATAATCAGGATCGCCAATAAAACTCAACGTTATCCGCGCCTGTTGATTACCGTTAGAAACCTGAATATCAGAGAGCGTCGCGGCACCCACCGGCGTGCACAG                                                                                                                                                            >  1:2706322/1‑149   TGTTTGATATCGAGCGCCACGGTGCGTTTGCTTTGATGGCTAAACGCATAATCAGGATCGCCAATAAAACTCAACGTTATCCGCGCCTGTTGATTACCGTTAGAAACCTGAATATCAGAGAGCGTCGCGGCACCCACCGGCGTGCACAG                                                                                                                                                            >  1:4498279/1‑149        GATATCGAGCGCCACGGTGCGTTTGCTTTGATGGCTAAACGCATAATCAGGATCGCCAATAAAACTCAACGTTATCCGCGCCTGTTGATTACCGTTAGAAACCTGAATATCAGAGAGCGTCGCGGCACCCACCGGCGTGCACCGCAGCA                                                                                                                                                       >  2:4554951/1‑149        GATATCGAGCGCCACGGTGCGTTTGCTTTGATGGCTAAACGCATAATCAGGATCGCCAATAAAACTCAACGTTATCCGCGCCTGTTGATTACCGTTAGAAACCTGAATATCAGAGAGCGTCGCGGCACCCACCGGCGTGCACAGCAGCA                                                                                                                                                       >  2:4556434/1‑149                                         GCTAAACGCATAATCAGGATCGCCAATAAAACTCAACGTTATCCGCGCCTGTTGATTACCGTTAGAAACCTGAATATCAGAGAGCGTCGCGGCACCCACCGGCGTGCACAGCAGCAGCGTCGCTACCAACCAATTTCTGATGCGATACA                                                                                                                      >  2:2854426/1‑149                                                                                                                               TCGCGGCACCCACCGGCGTGCACAGCAGCAGCGTCGCTACCAACCAATTTCTGATGCGATACATCATCCCGCCACCTTTCAAAGGTTAACCGGCTAAACGCGCCAGCAACAATTCACCCGCAGAGGAAACCGCACTCACGCGCGCCTCA                                >  2:249628/1‑149                                                                                                                                       CCCACCGGCGTGCACAGCAGCAGCGTCGCTACCAACCAATTTCTGATGCGATACATCATCCCGCCACCTTTCAAAGGTTAACCGGCTAAACGCGCCAGCAACAATTCACCCGCAGAGGAAACCGCACTCACGCGCGCCTCACGGCCTTG                        <  1:2854426/149‑1                                                                                                                                                      AGCAGCAGCGTCGCTACCAACCAATTTCTGATGCGATACATCATCCCGCCACCTTTCAAAGGTTAACCGGCTAAACGCGCCAGCAACAATTCACCCGCAGAGGAAACCGCACTCACGCGCGCCTCACGGCCTTGTGCCTGATAATCAAT         <  1:1879264/149‑1                                                                                                                                                      AGCAGCAGCGTCGCTACCAACCAATTTCTGATGCGATACATCATCCCGCCACCTTTCAAAGGTTAACCGGCTAAACGCGCCAGCAACAATTCACCCGCAGAGGAAACCGCACTCACGCGCGCCTCACGGCCTTGTGCCTGATAATCAAT         <  1:58651/149‑1                                                                                                                                                       GCAGCAGCGTCGCTACCAACCAATTTCTGATGCGATACATCATCCCGCCACCTTTCAAAGGTTAACCGGCTAAACGCGCCAGCAACAATTCACCCGCAGAGGAAACCGCACTCACGCGCGCCTCACGGCCTTGTGCCTGATAATCAATG        <  2:927600/149‑1                                                                                                                                                       GCAGCAGCGTCGCTACCAACCAATTTCTGATGCGATACATCATCCCGCCACCTTTCAAAGGTTAACCGGCTAAACGCGCCAGCAACAATTCACCCGCAGAGGAAACCGCACTCACGCGCGCCTCACGGCCTTGTGCCTGATAATCAATG        >  1:1319663/1‑149                                                                                                                                                       GCAGCAGCGTCGCTACCAACCAATTTCTGATGCGATACATCATCCCGCCACCTTTCAAAGGTTAACCGGCTAAACGCGCCAGCAACAATTCACCCGCAGAGGAAACCGCACTCACGCGCGCCTCACGGCCTTGTGCCTGATAATCAATG        <  2:1683505/149‑1                                                                                                                                                       GCAGCAGCGTCGCTACCAACCAATTTCTGATGCGATACATCATCCCGCCACCTTTCAAAGGTTAACCGGCTAAACGCGCCAGCAACAATTCACCCGCAGAGGAAACCGCACTCACGCGCGCCTCACGGCCTTGTGCCTGATAATCAATG        >  2:1574952/1‑149                                                                                                                                                       GCAGCAGCGTCGCTACCAACCAATTTCTGATGCGATACATCATCCCGCCACCTTTCAAAGGTTAACCGGCTAAACGCGCCAGCAACAATTCACCCGCAGAGGAAACCGCACTCACGCGCGCCTCACGGCCTTGTGCCTGATAATCAATG        <  1:745155/149‑1                                                                                                                                                       GCAGCAGCGTCGCTACCAACCAATTTCTGATGCGATACATCATCCCGCCACCTTTCAAAGGTTAACCGGCTAAACGCGCCAGCAACAATTCACCCGCAGAGGAAACCGCACTCACGCGCGCCTCACGGCCTTGTGCCTGATAATCAATG        >  1:2841151/1‑149                                                                                                                                                       GCAGCAGCGTCGCTACCAACCAATTTCTGATGCGATACATCATCCCGCCACCTTTCAAAGGTTAACCGGCTAAACGCGCCAGCAACAATTCACCCGCAGAGGAAACCGCACTCACGCGCGCCTCACGGCCTTGTGCCTGATAATCAATG        >  1:2513634/1‑149                                                                                                                                                       GCAGCAGCGTCGCTACCAACCAATTTCTGATGCGATACATCATCCCGCCACCTTTCAAAGGTTAACCGGCTAAACGCGCCAGCAACAATTCACCCGCAGAGGAAACCGCACTCACGCGCGCCTCACGGCCTTGTGCCTGATAATCAATG        >  1:2513614/1‑149                                                                                                                                                       GCAGCAGCGTCGCTACCAACCAATTTCTGATGCGATACATCATCCCGCCACCTTTCAAAGGTTAACCGGCTAAACGCGCCAGCAACAATTCACCCGCAGAGGAAACCGCACTCACGCGCGCCTCACGGCCTTGTGCCTGATAATCAATG        >  1:2513578/1‑149                                                                                                                                                         AGCAGCGTCGCTACCAACCAATTTCTGATGCGATACATCATCCCGCCACCTTTCAAAGGTTAACCGGCTAAACGCGCCAGCAACAATTCACCCGCAGAGGAAACCGCACTCACGCGCGCCTCACGGCCTTGTGCCTGATAATCAATGTG      <  1:2643201/149‑1                                                                                                                                                         AGCAGCGTCGCTACCAACCAATTTCTGATGCGATACATCATCCCGCCACCTTTCAAAGGTTAACCGGCTAAACGCGCCAGCAACAATTCACCCGCAGAGGAAACCGCACTCACGCGCGCCTCACGGCCTTGTGCCTGATAATCAATGTG      >  2:2982320/1‑149                                                                                                                                                         AGCAGCGTCGCTACCAACCAATTTCTGATGCGATACATCATCCCGCCACCTTTCAAAGGTTAACCGGCTAAACGCGCCAGCAACAATTCACCCGCAGAGGAAACCGCACTCACGCGCGCCTCACGGCCTTGTGCCTGATAATCAATGTG      <  1:2050365/149‑1                                                                                                                                                          GCAGCGTCGCTACCAACCAATTTCTGATGCGATACATCATCCCGCCACCTTTCCAAGGTTAACCGGCTAAACGCGCCAGCAACAATTCACCCGCAGAGGAAACCGCACTCACGCGCGCCTCACGGCCTTGTGCCTGATAATCAATGTGT     >  1:466033/1‑149                                                                                                                                                          GCAGCGTCGCTACCAACCAATTTCTGATGCGATACATCATCCCGCCACCTTTCAAAGGTTAACCGGCTAAACGCGCCAGCAACAATTCACCCGCAGAGGAAACCGCACTCACGCGCGCCTCACGGCCTTGTGCCGGATAATCAATGTGT     >  1:465425/1‑149                                                                                                                                                            AGCGTCGCTACCAACCAATTTCTGATGCGATACATCATCCCGCCACCTTTCAAAGGTTAACCGGCTAAACGCGCCAGCAACAATTCACCCGCAGAGGAAACCGCACTCACGCGCGCCTCACGGCCTTGTGCCTGATAATCAATGTGTAT   <  2:1884964/149‑1                                                                                                                                                             GCGTCGCTACCAACCAATTTCTGATGCGATACATCATCCCGCCACCTTTCAAAGGTTAACCGGCTAAACGCGCCAGCAACAATTCACCCGCAGAGGAAACCGCACTCACGCGCGCCTCACGGCCTTGTGCCTGATAATCAATGTGTATT  >  2:1201357/1‑149                                                                                                                                                             GCGTCGCTACCAACCAATTTCTGATGCGATACATCATCCCGCCACCTTTCAAAGGTTAACCGGCTAAACGCGCCAGCAACAATTCACCCGCAGAGGAAACCGCACTCACGCGCGCCTCACGGCCTTGTGCCTGATAATCAATGTGTATT  >  1:4486753/1‑149                                                                                                                                                             GCGTCGCTACCAACCAATTTCTGATGCGATACATCATCCCGCCACCTTTCAAAGGTTAACCGGCTAAACGCGCCAGCAACAATTCACCCGCAGAGGAAACCGCACTCACGCGCGCCTCACGGCCTTGTGCCTGATAATCAATGTGTATT  >  1:283798/1‑149                                                                                                                                                             GCGTCGCTACCAACCAATTTCTGATGCGATACATCATCCCGCCACCTTTCAAAGGTTAACCGGCTAAACGCGCCAGCAACAATTCACCCGCAGAGGAAACCGCACTCACGCGCGCCTCACGGCCTTGTGCCTGATAATCAATGTGTATT  >  2:2789377/1‑149                                                                                                                                                                                                                                                                                                                   TTTGTTTGATATCGAGCGCCACGGTGCGTTTGCTTTGATGGCTAAACGCATAATCAGGATCGCCAATAAAACTCAACGTTATCCGCGCCTGTTGATTACCGTTAGAAACCTGAATATCAGAGAGCGTCGCGGCACCCACCGGCGTGCACAGCAGCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP009273/4386077‑4385920 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cagcagcagcGTCGCTACCAACCAATTTCTGATGCGATACATCATCCCGCCACCTTTCAAAGGTTAACCGGCTAAACGCGCCAGCAACAATTCACCCGCAGAGGAAACCGCACTCACGCGCGCCTCACGGCCTTGTGCCTGATAATCAATGTGTATT  <  CP009273/4385916‑4385770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.