breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 39 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,165,1840TG56.2% 3.0 / 14.2 20intergenic (+137/+46)puuB_6/fadD_1Gamma‑glutamylputrescine oxidoreductase/Long‑chain‑fatty‑acid‑‑CoA ligase
*ACB1223,491,3370GC56.0% 20.4 / 34.4 25intergenic (‑699/+22)CJ019_03306/CJ019_03307hypothetical protein/hypothetical protein
*ACB1225,688,6370AC54.8% 2.1 / 16.9 31S236R (AGC→CGC) pyrC'Dihydroorotase‑like protein
*ACB1223,440,3480TG54.2% 1.4 / 12.6 28A19A (GCT→GCGCJ019_03266hypothetical protein
*ACB1225,974,0620TG54.2% 19.0 / 29.8 38L684R (CTG→CGG) sasA_19Adaptive‑response sensory‑kinase SasA
*ACB1223,440,3630AC53.8% 6.5 / 21.6 27A24A (GCA→GCCCJ019_03266hypothetical protein
*ACB1222,856,8650TG52.9% 5.2 / 14.1 17W290G (TGG→GGG) hdfR_4HTH‑type transcriptional regulator HdfR
*ACB1222,856,8720TG52.9% 7.4 / 14.8 17L292W (TTG→TGG) hdfR_4HTH‑type transcriptional regulator HdfR
*ACB1223,626,0790AC52.2% 9.8 / 22.1 23N165T (AAC→ACC) hcf136_3Ycf48‑like protein
*ACB1223,229,2020AC52.0% 1.1 / 11.9 25T414P (ACC→CCC) garD_1Galactarate dehydratase (L‑threo‑forming)
*ACB1225,688,6450TG51.4% 10.8 / 24.1 35R238R (CGT→CGGpyrC'Dihydroorotase‑like protein
*ACB1224,385,0020GC50.0% 1.5 / 10.0 18intergenic (‑374/+96)CJ019_00698/CJ019_04138hypothetical protein/hypothetical protein
*ACB1225,684,6340AC47.2% 28.6 / 11.5 36P118P (CCA→CCCCJ019_05336hypothetical protein
*ACB1222,780,7060AT46.4% 2.5 / 14.6 28intergenic (+96/‑29)ahpC_2/ahpFAlkyl hydroperoxide reductase C/Alkyl hydroperoxide reductase subunit F
*ACB1221,157,9830AC44.9% 33.9 / 11.5 49S162A (TCG→GCG) hexR_1HTH‑type transcriptional regulator HexR
*ACB1222,679,3570TG43.2% 47.0 / 14.8 45E474D (GAA→GACrcsC_4Sensor histidine kinase RcsC
*ACB122411,3390CA37.0% 56.9 / 10.1 47F24L (TTC→TTAkdkA_13‑deoxy‑D‑manno‑octulosonic acid kinase
*ACB1225,525,8830AC36.7% 62.6 / 10.3 49intergenic (‑53/+102)livH_5/braC_2High‑affinity branched‑chain amino acidtransport system permease protein LivH/Leucine‑, isoleucine‑, valine‑, threonine‑, and alanine‑binding protein
*ACB122502,3280TG35.2% 97.7 / 10.7 71intergenic (‑268/‑100)estP/trpGEsterase EstP/Anthranilate synthase component 2
*ACB1221,482,0140TG34.7% 59.6 / 10.3 49V173G (GTG→GGG) CJ019_01397GTP cyclohydrolase 1 type 2

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? ACB122 1734821 =75 (1.090)3 (0.050) 3/236 6.8 4.3% coding (148/753 nt) CJ019_01633 hypothetical protein
?ACB122 = 5091718 63 (0.990)coding (1513/1962 nt) acs Acetyl‑coenzyme A synthetase