breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 874,860 G→A M221I (ATG→ATA yliI → aldose sugar dehydrogenase YliI
RA 1,431,900 G→A Q1048* (CAG→TAG)  nifJ ← pyruvate:ferredoxin (flavodoxin) oxidoreductase
MC JC 3,632,439 Δ6 bp coding (1438‑1443/1500 nt) pitA → inorganic phosphate transporter PitA
RA 3,962,397 C→T Q3* (CAA→TAA)  wzzE → ECA polysaccharide chain length modulation protein
RA 4,477,499 A→G E122G (GAA→GGA)  lptG → LPS export ABC transporter permease LptG

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 236741 270746–269666 32926–34006 137 [2] [53] 54 ivy–[BW25113_RS01325] ivy,fadE,lpcA,yafJ,dpaA,yafQ,dinJ,yafL,rayT,BW25113_RS01160,lafU,dinB,yafN,yafO,yafP,BW25113_RS01190,prfH,pepD,gpt,frsA,crl,phoE,proB,proA,BW25113_RS01235,ykfI,yafW,ykfH,ykfG,yafX,ykfF,BW25113_RS01265,BW25113_RS25425,BW25113_RS25430,BW25113_RS25435,yafZ,ykfA,perR,BW25113_RS01295,BW25113_RS01300,BW25113_RS01305,ykfC,[BW25113_RS01325]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 = 2367402 (0.020)134 (1.380) 104/494 0.0 98.5% intergenic (‑64/‑117) yafV/ivy 2‑oxoglutaramate amidase/Ivy family C‑type lysozyme inhibitor
?NZ_CP009273 269666 = NA (NA)intergenic (+2/+146) ykfC/BW25113_RS01325 reverse transcriptase domain‑containing protein/IS5‑like element IS5 family transposase
* ? NZ_CP009273 1203246 =14 (0.140)62 (0.680) 60/462 1.2 78.9% coding (290/630 nt) BW25113_RS05990 protein StfP
?NZ_CP009273 1205075 = 20 (0.220)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 120326114 (0.140)71 (0.780) 66/462 0.8 81.1% coding (305/630 nt) BW25113_RS05990 protein StfP
?NZ_CP009273 = 1205058 20 (0.220)coding (57/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 1911494 =2 (0.020)91 (0.930) 75/494 0.6 97.9% coding (980/1284 nt) yebS membrane integrity lipid transport subunit YebS
?NZ_CP009273 2095230 = NA (NA)pseudogene (1/354 nt) wbbL N‑acetylglucosaminyl‑diphospho‑decaprenol L‑rhamnosyltransferase WbbL
* ? NZ_CP009273 = 19114972 (0.020)139 (1.430) 104/494 0.0 98.6% coding (983/1284 nt) yebS membrane integrity lipid transport subunit YebS
?NZ_CP009273 = 2283592 NA (NA)intergenic (‑68/‑387) BW25113_RS11435/narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP
* ? NZ_CP009273 2262322 =3 (0.030)99 (1.020) 92/494 0.1 97.1% intergenic (+28/‑11) yeiR/lpxT zinc‑binding GTPase YeiR/Kdo(2)‑lipid A phosphotransferase
?NZ_CP009273 = 2283592 NA (NA)intergenic (‑68/‑387) BW25113_RS11435/narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP
* ? NZ_CP009273 = 22623250 (0.000)87 (0.890) 77/494 0.5 100% intergenic (+31/‑8) yeiR/lpxT zinc‑binding GTPase YeiR/Kdo(2)‑lipid A phosphotransferase
?NZ_CP009273 2282398 = NA (NA)pseudogene (3/2532 nt) yejO autotransporter YejO