breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 197,270 T→C F101S (TTT→TCT)  skp → molecular chaperone Skp
RA 248,997 T→C F70F (TTT→TTC yafO → type II toxin‑antitoxin system mRNA interferase toxin YafO
RA 791,492 C→T P59S (CCC→TCC)  modB → molybdate ABC transporter permease subunit
RA 874,860 G→A M221I (ATG→ATA yliI → aldose sugar dehydrogenase YliI
RA 1,431,900 G→A Q1048* (CAG→TAG)  nifJ ← pyruvate:ferredoxin (flavodoxin) oxidoreductase
RA 1,446,404 A→G V499A (GTT→GCT)  paaZ ← phenylacetic acid degradation bifunctional protein PaaZ
RA 1,865,003 C→A T121K (ACA→AAA)  cdgI → GGDEF domain‑containing protein
RA 2,961,644 G→T Y50* (TAC→TAA ptsP ← phosphoenolpyruvate‑‑protein phosphotransferase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 387326–388421 397243 8823–9918 17 [16] [0] 54 [BW25113_RS01920]–[phoA] [BW25113_RS01920],BW25113_RS01920,BW25113_RS01930,iprA,ampH,sbmA,yaiW,yaiY,yaiZ,ddlA,BW25113_RS25915,iraP,[phoA]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 360104 =27 (0.450)29 (0.490) 27/496 1.7 46.4% intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 40 (0.670)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 = 388422NA (NA)54 (0.910) 50/494 0.3 100% intergenic (‑65/‑7) BW25113_RS01920/BW25113_RS01930 IS3‑like element IS3 family transposase/autotransporter outer membrane beta‑barrel domain‑containing protein
?NZ_CP009273 397244 = 0 (0.000)coding (42/1416 nt) phoA alkaline phosphatase
* ? NZ_CP009273 = 1090958NA (NA)77 (1.300) 66/494 0.1 98.7% intergenic (‑65/‑3) BW25113_RS05345/BW25113_RS05355 IS3‑like element IS3 family transposase/ACP S‑malonyltransferase
?NZ_CP009273 = 3794169 1 (0.020)coding (157/699 nt) rfaY lipopolysaccharide core heptose(II) kinase RfaY
* ? NZ_CP009273 = 2164908NA (NA)69 (1.170) 61/494 0.1 100% intergenic (+29/+1) BW25113_RS10900/BW25113_RS10905 IS3‑like element IS3 family transposase/DeoR family transcriptional regulator
?NZ_CP009273 3794166 = 0 (0.000)coding (160/699 nt) rfaY lipopolysaccharide core heptose(II) kinase RfaY