breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 708,896 (AACACC)2→1 coding (454‑459/546 nt) seqA → replication initiation negative regulator SeqA
JC 767,825 Δ4 bp coding (912‑915/1569 nt) cydA → cytochrome ubiquinol oxidase subunit I
RA 874,860 G→A M221I (ATG→ATA yliI → aldose sugar dehydrogenase YliI
RA 1,431,900 G→A Q1048* (CAG→TAG)  nifJ ← pyruvate:ferredoxin (flavodoxin) oxidoreductase
MC JC 1,909,389 Δ5 bp coding (399‑403/699 nt) proQ ← RNA chaperone ProQ
MC JC 1,985,958 Δ3 bp coding (339‑341/549 nt) pgsA ← CDP‑diacylglycerol‑‑glycerol‑3‑phosphate 3‑phosphatidyltransferase
RA 3,275,282 C→A intergenic (+298/‑53) BW25113_RS16255 → / → agaS amidohydrolase family protein/AgaS family sugar isomerase
RA 3,794,560 C→A G267V (GGT→GTT)  rfaJ ← lipopolysaccharide 1,2‑glucosyltransferase RfaJ
RA 3,800,777 C→A E240* (GAA→TAA)  rfaQ ← lipopolysaccharide core heptosyltransferase RfaQ

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 360104 =46 (0.500)44 (0.480) 38/496 1.8 46.6% intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 55 (0.600)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 1203246 =12 (0.130)57 (0.660) 53/464 0.8 87.5% coding (290/630 nt) BW25113_RS05990 protein StfP
?NZ_CP009273 1205075 = 5 (0.060)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 120326112 (0.130)37 (0.430) 35/464 1.8 82.0% coding (305/630 nt) BW25113_RS05990 protein StfP
?NZ_CP009273 = 1205058 5 (0.060)coding (57/540 nt) BW25113_RS06005 phage tail protein