breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 56,162 G→T N316K (AAC→AAA lptD ← LPS assembly protein LptD
RA 103,231 G→A C63Y (TGT→TAT)  lpxC → UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase
RA 874,860 G→A M221I (ATG→ATA yliI → aldose sugar dehydrogenase YliI
RA 914,598 +T intergenic (‑58/‑93) ybjX ← / → macA VirK/YbjX family protein/macrolide transporter subunit MacA
RA 1,431,900 G→A Q1048* (CAG→TAG)  nifJ ← pyruvate:ferredoxin (flavodoxin) oxidoreductase
RA 4,322,729 C→T E124K (GAA→AAA)  pmrB ← two‑component system sensor histidine kinase PmrB
RA 4,540,670 T→A A148A (GCA→GCT gntP ← gluconate permease GntP

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 360104 =48 (0.480)68 (0.670) 54/496 2.3 57.4% intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 53 (0.530)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 = 1391495NA (NA)87 (0.860) 78/496 0.6 97.8% intergenic (+146/‑127) BW25113_RS06980/ynaJ IS5‑like element IS5 family transposase/DUF2534 family protein
?NZ_CP009273 = 1796931 2 (0.020)intergenic (‑104/‑420) thrS/espL1 threonine‑‑tRNA ligase/type III secretion system effector EspL1
* ? NZ_CP009273 1796928 =0 (0.000)93 (0.920) 80/496 0.5 100% intergenic (‑101/‑423) thrS/espL1 threonine‑‑tRNA ligase/type III secretion system effector EspL1
?NZ_CP009273 = 2096424 NA (NA)intergenic (‑68/+3) BW25113_RS10605/wbbL IS5‑like element IS5 family transposase/N‑acetylglucosaminyl‑diphospho‑decaprenol L‑rhamnosyltransferase WbbL
* ? NZ_CP009273 = 2283592NA (NA)142 (1.410) 111/496 0.0 57.0% intergenic (‑68/‑387) BW25113_RS11435/narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP
?NZ_CP009273 = 3179496 107 (1.060)intergenic (+172/‑50) ygiL/BW25113_RS15805 fimbrial‑like protein/IS3‑like element IS2 family transposase
* ? NZ_CP009273 3645396 =NA (NA)115 (1.140) 95/496 0.1 96.6% pseudogene (746/747 nt) BW25113_RS18155 hypothetical protein
?NZ_CP009273 = 3800969 4 (0.040)coding (526/1071 nt) rfaQ lipopolysaccharide core heptosyltransferase RfaQ
* ? NZ_CP009273 = 3646590NA (NA)106 (1.050) 89/496 0.2 99.1% pseudogene (3/379 nt) BW25113_RS18165 hypothetical protein
?NZ_CP009273 3800966 = 1 (0.010)coding (529/1071 nt) rfaQ lipopolysaccharide core heptosyltransferase RfaQ