breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsSRR12849358_R1.good.fq1,263,197314,532,527100.0%249.0 bases249 bases100.0%
errorsSRR12849358_R2.good.fq1,263,197314,532,527100.0%249.0 bases249 bases99.6%
total2,526,394629,065,054100.0%249.0 bases249 bases99.8%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionBA0000363,309,401202.51.7100.0%Corynebacterium glutamicum ATCC 13032 DNA, complete genome.
total3,309,401100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 100000167
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 2
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 50004
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.001

Junction Skew Score Calculation

reference sequencepr(no read start)
BA0000360.70527

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction0.1
Junction allow suboptimal matchesFALSE
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 3
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 1

Read Alignment Evidence

optionvalue
ModeConsensus/Mixed Base
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff10
Polymorphism frequency cutoff0.2
Polymorphism minimum variant coverage each strandOFF
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs ofOFF
Skip base substitutions when they create a homopolymer flanked on each side byOFF

Software Versions

programversion
bowtie22.2.6
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input13:38:46 13 Feb 202313:39:48 13 Feb 20231 minute 2 seconds
Read alignment to reference genome13:39:48 13 Feb 202313:50:23 13 Feb 202310 minutes 35 seconds
Preprocessing alignments for candidate junction identification13:50:23 13 Feb 202313:51:00 13 Feb 202337 seconds
Preliminary analysis of coverage distribution13:51:00 13 Feb 202313:52:51 13 Feb 20231 minute 51 seconds
Identifying junction candidates13:52:51 13 Feb 202313:52:51 13 Feb 20230 seconds
Re-alignment to junction candidates13:52:51 13 Feb 202313:54:53 13 Feb 20232 minutes 2 seconds
Resolving best read alignments13:54:53 13 Feb 202313:55:47 13 Feb 202354 seconds
Creating BAM files13:55:47 13 Feb 202313:57:20 13 Feb 20231 minute 33 seconds
Tabulating error counts13:57:20 13 Feb 202313:58:16 13 Feb 202356 seconds
Re-calibrating base error rates13:58:16 13 Feb 202313:58:17 13 Feb 20231 second
Examining read alignment evidence13:58:17 13 Feb 202314:09:23 13 Feb 202311 minutes 6 seconds
Polymorphism statistics14:09:23 13 Feb 202314:09:23 13 Feb 20230 seconds
Output14:09:23 13 Feb 202314:09:35 13 Feb 202312 seconds
Total 30 minutes 49 seconds