breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 23 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252681,292,8480AT72.3% 229.1 / 193.0 304noncoding (11/132 nt)CXP41_RS06830RtT sRNA
*NZ_CP0252681,293,1600GA72.0% 182.8 / 74.5 136noncoding (55/132 nt)CXP41_RS06840RtT sRNA
*NZ_CP0252681,293,0000AG63.5% 148.4 / 259.7 255noncoding (37/132 nt)CXP41_RS06835RtT sRNA
*NZ_CP0252681,292,9910CT63.3% 185.5 / 245.5 274noncoding (46/132 nt)CXP41_RS06835RtT sRNA
*NZ_CP0252681,292,9820AG53.0% 35.6 / inf 272noncoding (55/132 nt)CXP41_RS06835RtT sRNA
*NZ_CP0252684,621,3850CT48.9% 174.5 / inf 1506A414V (GCG→GTG) deoAthymidine phosphorylase
*NZ_CP0252681,293,1690CT30.1% 150.3 / 108.4 138noncoding (46/132 nt)CXP41_RS06840RtT sRNA
*NZ_CP0252682,474,4700CA29.9% 123.6 / 11.7 87Q436K (CAA→AAA) CXP41_RS13000hypothetical protein
*NZ_CP0252681,293,1780AG29.8% 184.6 / 85.6 139noncoding (37/132 nt)CXP41_RS06840RtT sRNA
*NZ_CP0252682,474,4610CA29.3% 87.5 / 16.6 82Q433K (CAA→AAA) CXP41_RS13000hypothetical protein
*NZ_CP025268164,4120AC29.2% 169.7 / 19.9 130intergenic (+47/‑149)hrpB/mrcBATP‑dependent helicase HrpB/bifunctional glycosyl transferase/transpeptidase
*NZ_CP0252681,292,8870CA24.3% 439.8 / 208.3 385intergenic (‑29/+18)CXP41_RS06830/CXP41_RS06835RtT sRNA/RtT sRNA
*NZ_CP0252683,505,5530TA23.8% 476.0 / 39.4 240intergenic (+84/+68)phoB/yhfSDNA‑binding transcriptional regulator FrlR/aminotransferase class I/II‑fold pyridoxal phosphate‑dependent enzyme
*NZ_CP025268922,3060AT23.4% 556.6 / 70.5 290intergenic (‑342/+153)lysO/aqpZL‑lysine exporter LysO/aquaporin Z
*NZ_CP0252682,233,2390TA23.3% 315.4 / 41.3 167intergenic (+139/+234)yohP/dusCsmall membrane protein YohP/tRNA dihydrouridine(16) synthase DusC
*NZ_CP0252681,292,8830AG23.0% 519.1 / 200.1 378intergenic (‑25/+22)CXP41_RS06830/CXP41_RS06835RtT sRNA/RtT sRNA
*NZ_CP0252681,292,8910GA22.4% 485.2 / 223.5 375intergenic (‑33/+14)CXP41_RS06830/CXP41_RS06835RtT sRNA/RtT sRNA
*NZ_CP025268164,3950AT21.9% 281.3 / 17.2 145intergenic (+30/‑166)hrpB/mrcBATP‑dependent helicase HrpB/bifunctional glycosyl transferase/transpeptidase
*NZ_CP0252683,505,5590TA21.4% 462.6 / 48.7 234intergenic (+90/+62)phoB/yhfSDNA‑binding transcriptional regulator FrlR/aminotransferase class I/II‑fold pyridoxal phosphate‑dependent enzyme
*NZ_CP0252682,233,2480GC20.9% 419.4 / 57.7 182intergenic (+148/+225)yohP/dusCsmall membrane protein YohP/tRNA dihydrouridine(16) synthase DusC

Marginal new junction evidence (lowest skew 10 of 510 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP025268 1214891 =602 (1.070)114 (0.230) 67/244 2.4 19.4% coding (290/630 nt) CXP41_RS06370 protein StfP
?NZ_CP025268 1216719 = 414 (0.830)coding (40/540 nt) CXP41_RS06385 phage tail protein
* ? NZ_CP025268 66565 =223 (0.400)135 (0.320)
+35 bp
50/206 3.0 44.0% intergenic (‑14/+101) araD/araA L‑ribulose‑5‑phosphate 4‑epimerase/L‑arabinose isomerase
?NZ_CP025268 = 66614 237 (0.420)intergenic (‑63/+52) araD/araA L‑ribulose‑5‑phosphate 4‑epimerase/L‑arabinose isomerase
* ? NZ_CP025268 1292725 =486 (0.860)143 (0.300) 55/234 3.3 36.6% intergenic (+182/+2) narI/CXP41_RS06830 respiratory nitrate reductase subunit gamma/RtT sRNA
?NZ_CP025268 = 1293258 84 (0.180)intergenic (‑44/+3) CXP41_RS06840/CXP41_RS06845 RtT sRNA/tRNA‑Tyr
* ? NZ_CP025268 695911 =0 (0.000)276 (0.540)
+GAAGAAACAATC
52/252 4.1 54.0% intergenic (‑33/+2) CXP41_RS03680/CXP41_RS03685 tRNA‑Gln/tRNA‑Gln
?NZ_CP025268 = 696182 515 (0.910)intergenic (‑1/+379) CXP41_RS03695/asnB tRNA‑Met/asparagine synthase B
* ? NZ_CP025268 4544596 =63 (0.110)84 (0.160) 42/258 5.7 49.0% intergenic (+49/‑433) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP025268 4544910 = 116 (0.220)intergenic (+363/‑119) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
* ? NZ_CP025268 = 454460451 (0.090)78 (0.150) 38/258 6.4 49.1% intergenic (+57/‑425) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP025268 = 4544900 114 (0.220)intergenic (+353/‑129) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
* ? NZ_CP025268 = 3482513420 (0.750)29 (0.070) 23/214 8.1 8.2% coding (539/1914 nt) yheS ABC transporter ATP‑binding protein
?NZ_CP025268 3483357 = 325 (0.740)coding (1383/1914 nt) yheS ABC transporter ATP‑binding protein
* ? NZ_CP025268 = 780371417 (0.740)26 (0.060) 20/198 8.3 9.8% intergenic (+100/‑47) CXP41_RS04105/CXP41_RS04110 tRNA‑Lys/tRNA‑Lys
?NZ_CP025268 780860 = 180 (0.450)intergenic (+3/‑274) CXP41_RS04120/nadA RtT sRNA/quinolinate synthase NadA
* ? NZ_CP025268 = 4293125646 (1.150)25 (0.050) 22/250 9.6 4.4% coding (727/957 nt) nrfD cytochrome c nitrite reductase subunit NrfD
?NZ_CP025268 4294017 = 501 (0.980)coding (643/1719 nt) nrfE heme lyase CcmF/NrfE family subunit
* ? NZ_CP025268 = 684590705 (1.250)27 (0.050) 22/252 9.7 4.2% coding (388/675 nt) gltK glutamate/aspartate ABC transporter permease GltK
?NZ_CP025268 685289 = 590 (1.150)coding (429/741 nt) gltJ glutamate/aspartate ABC transporter permease GltJ