New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 = 2293898300 (1.200)11 (0.050)
+TACGGCTCGGATTT		 3/122 16.6 4.1% intergenic (+48/‑194) nrdA/nrdB ribonucleotide‑diphosphate reductase alpha subunit/ribonucleotide‑diphosphate reductase beta subunit
?REL606 2374569 = 341 (1.370)intergenic (‑214/+45) cvpA/dedD membrane protein required for colicin V production/hypothetical protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCGGCGCATGTAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/2293831‑2293898
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAA  >  REL606/2374569‑2374606
                                                                    ||||||||||||||                                      
GCGGCGCATGTACGCTCTGGTTTCGGGGTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCTACGGCTC                                              <  1:902835/76‑1
                                 gtTGCAGCGTAAACCCTTTATCGGGCCTACGGCTCTACGGCTCGGATTTGTAGGCCTGATAAGACGCGCCAGCGTC             <  2:7072190/74‑1
                                 GATGCGGCGTGAACGCCTTATCCGGCCTACGGCTCTACGGCTCGGATTTGTAGGCCTGATAAGACGCGCCAGCGTC             <  2:2804595/76‑1
                                 GATGCGGCGTGAACGCCTTATCCGGCCTACGGCTCTACGGCTCGGATTTGTAGGCCTGATAAGACGCGCCAGCGTC             <  2:3679171/76‑1
                                 GATGCAGCGTAAACGCCTTATCCGGCCTACGGCTCTACGGCTCGGATTTGTAGGCCTGATAAGACGCGCCAGCGTC             <  2:3517604/76‑1
                                   TGCAGCGTAAACGCCTTATCCGGCCTACGGCTCTACGGCTCGGATTTGTAGGCCTGATAAGACGCGCCAGCGTCGC           <  2:4918993/76‑1
                                    GCGGCGTAACCCCCTTATCCGGCCTACGGCTCTACGGCTCGGATTTGTAGGCCTGATAAGACGCGCCAGCGTCGCA          <  2:4296700/76‑1
                                     caGCGTAACCCCCTTATCCGGCCTACGGCTCTACGGCTCGGATTTGTAGGCCTGATAAGACGCGCCAGCGTCGCAT         <  2:3087057/74‑1
                                      aGCGTAAACGCCTTATCCGGCCTACGGCTCTACGGCTCGGATTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATC        <  2:6090112/75‑1
                                           gacCGCTTTACCCGCCCTACGGCTCTACGGCTCGGATTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCA   <  2:4574055/73‑1
                                           gacCGCCTTACCCGGCCTACGGCTCTACGGCTCGGATTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCA   <  2:6962212/73‑1
                                            AACGCCTTATCCGGCCTACGGCTCTACGGCTCCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGgcc  >  2:6494733/1‑73
                                                                                 TGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGC    >  1:5893204/1‑37
                                                                                 TGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGC    <  2:5893204/37‑1
                                                                                 TGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGG     >  1:5834211/1‑36
                                                                                 TGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGG     <  2:5834211/36‑1
                                                                    ||||||||||||||                                      
GCGGCGCATGTAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/2293831‑2293898
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAA  >  REL606/2374569‑2374606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.