New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 = 2293898300 (1.200)11 (0.050)
+TACGGCTCGGATTT
3/122 16.6 4.1% intergenic (+48/‑194) nrdA/nrdB ribonucleotide‑diphosphate reductase alpha subunit/ribonucleotide‑diphosphate reductase beta subunit
?REL606 2374569 = 341 (1.370)intergenic (‑214/+45) cvpA/dedD membrane protein required for colicin V production/hypothetical protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

Only 100 of 350 total aligned reads displayed.

ATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCA  >  REL606/2374494‑2374644
                                                                           |                                                                           
aTGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCg                                                                             >  2:7644129/1‑76 (MQ=255)
 tGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGt                                                                            <  2:3220227/76‑1 (MQ=255)
  gCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTa                                                                           <  2:4183673/76‑1 (MQ=255)
  gCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTa                                                                           >  2:5583004/1‑76 (MQ=255)
    cTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAgg                                                                         <  2:3692268/76‑1 (MQ=255)
    cTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAgg                                                                         <  1:2687508/76‑1 (MQ=255)
       tGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGCCCTACGGTTCTACCCCTGCGTAGGCCt                                                                      >  2:5496083/1‑76 (MQ=255)
       tGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCt                                                                      >  2:4711974/1‑76 (MQ=255)
       tGCAGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCt                                                                      >  1:7824696/1‑76 (MQ=255)
           gCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTAGCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATa                                                                  <  1:1031061/76‑1 (MQ=255)
            cATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATaa                                                                 >  2:5217908/1‑76 (MQ=255)
             aTCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAg                                                                <  1:1508314/76‑1 (MQ=255)
              tCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGa                                                               >  2:1144902/1‑76 (MQ=255)
                gggCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGAcg                                                             >  1:2835719/1‑76 (MQ=255)
                gggCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGAcg                                                             >  2:5669796/1‑76 (MQ=255)
                gggCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGAcg                                                             >  2:7704534/1‑76 (MQ=255)
                  gCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGAcgcg                                                           >  2:4316547/1‑76 (MQ=255)
                   cAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACgcgc                                                          >  2:8222872/1‑76 (MQ=255)
                    aaTGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGcc                                                         >  2:569382/1‑76 (MQ=255)
                    aaTGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGcc                                                         <  1:5612770/76‑1 (MQ=255)
                     aTGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCa                                                        <  2:6181977/76‑1 (MQ=255)
                     aTGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCa                                                        <  2:3474880/76‑1 (MQ=255)
                     aTGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCa                                                        >  2:6988284/1‑76 (MQ=255)
                        cGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCg                                                     >  1:432541/1‑76 (MQ=255)
                        cGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCg                                                     >  2:6267069/1‑76 (MQ=255)
                         gtgtCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGt                                                    <  1:4134165/76‑1 (MQ=255)
                         gtgtCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGt                                                    <  2:6056132/76‑1 (MQ=255)
                          tgtCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTc                                                   >  2:6415507/1‑76 (MQ=255)
                          tgtCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTc                                                   <  2:2281006/76‑1 (MQ=255)
                          tgtCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTc                                                   <  2:2157505/76‑1 (MQ=255)
                          gtgcGGAGGCGGCTTAAACGCCGTATCCTACCTACGGTGCTACCCCTCCGTCGGCCTGATAAGACGCGCCAGCGTc                                                   <  2:7384572/73‑1 (MQ=21)
                           gtCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCg                                                  <  1:1114867/76‑1 (MQ=255)
                            tCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGc                                                 >  2:3272252/1‑76 (MQ=255)
                              ggATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCAt                                               <  2:365997/76‑1 (MQ=255)
                               gATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATc                                              >  2:292052/1‑76 (MQ=255)
                               gATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATc                                              <  2:2124781/76‑1 (MQ=255)
                                gtGCGGCGTAAACGCCTTATCCGACCTACGGTTCTCCCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCa                                             <  2:1686931/75‑1 (MQ=255)
                                aTGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCa                                             <  1:2931569/76‑1 (MQ=255)
                                aTGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCa                                             >  2:3770549/1‑76 (MQ=255)
                                aTGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCa                                             <  1:4964278/76‑1 (MQ=255)
                                 tGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAg                                            <  2:6535041/76‑1 (MQ=255)
                                 tGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAg                                            <  1:1343215/76‑1 (MQ=255)
                                  gcggcgTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAgg                                           <  1:810394/76‑1 (MQ=255)
                                   cggcgTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGc                                          <  1:8236673/76‑1 (MQ=255)
                                   cggcgTAAACGCCTTAGCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGc                                          <  2:7991394/76‑1 (MQ=255)
                                    ggcgTAACCGCCTTAACCGACCTAGGGTTCTCCCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCa                                         <  2:3543656/76‑1 (MQ=21)
                                    ggcgTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCa                                         <  2:1291610/76‑1 (MQ=255)
                                     gcgTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCaa                                        <  2:1736245/76‑1 (MQ=255)
                                      cgTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAg                                       >  1:3789991/1‑76 (MQ=255)
                                      cgTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAg                                       >  2:7491162/1‑76 (MQ=255)
                                      cgTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAg                                       <  1:6999112/76‑1 (MQ=255)
                                      cgTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAg                                       <  2:2569649/76‑1 (MQ=255)
                                       gTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGa                                      <  2:7094065/76‑1 (MQ=255)
                                       gTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGa                                      <  2:7824696/76‑1 (MQ=255)
                                       gTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGa                                      >  1:3293869/1‑76 (MQ=255)
                                       gTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGa                                      <  2:8120199/76‑1 (MQ=255)
                                        tAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGAc                                     <  2:1368694/76‑1 (MQ=255)
                                           aCGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGt                                  <  2:4112225/76‑1 (MQ=255)
                                            cGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTa                                 <  2:2494457/76‑1 (MQ=255)
                                             gCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTAt                                >  1:3071955/1‑76 (MQ=255)
                                             cccTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTAt                                <  2:5378130/75‑1 (MQ=255)
                                              ccTTATCCGACCTACGGTTCTCCCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTAtt                               <  2:6632656/76‑1 (MQ=255)
                                              ccTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTAtt                               <  2:6812554/76‑1 (MQ=255)
                                              ccTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTAtt                               >  2:2363658/1‑76 (MQ=255)
                                              ccTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTAtt                               >  2:722168/1‑76 (MQ=255)
                                              ccTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTAtt                               <  2:5109240/76‑1 (MQ=255)
                                              ccTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTAtt                               <  2:5899245/76‑1 (MQ=255)
                                               cTTATCCGACCTACGGTTCTCCCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTa                              <  2:4420611/76‑1 (MQ=255)
                                               cTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTa                              <  2:2645716/76‑1 (MQ=255)
                                               cTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTa                              <  2:7885042/76‑1 (MQ=255)
                                                 tATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAAt                            >  2:2748832/1‑76 (MQ=255)
                                                 tATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAAt                            >  1:3993389/1‑76 (MQ=255)
                                                    ccGACCTACGGTTCTACCCCTGCGTAGGGCTGATAAGACGCGCCAGCGTCGCACCAGGCAAGACCGTCTTAATTCg                         >  2:1831132/1‑76 (MQ=37)
                                                    ccGACCTACGGCTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCg                         >  1:3572962/1‑76 (MQ=255)
                                                        ccTAGGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGt                     <  2:87990/76‑1 (MQ=255)
                                                         cTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGta                    >  1:6047653/1‑76 (MQ=255)
                                                          tACGGTTCTCCCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGtat                   <  1:6557468/76‑1 (MQ=255)
                                                           aCGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGtata                  >  2:7562218/1‑76 (MQ=255)
                                                            cGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAg                 <  2:6345926/76‑1 (MQ=255)
                                                            cGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAg                 >  2:205887/1‑76 (MQ=255)
                                                             ggTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGc                <  2:3975981/76‑1 (MQ=255)
                                                              gTTCTCCCCCTGGGTGGCCCAGAAAGGCCGCGCCGGCGCCGCATAAGGCAAGACAGTATTAATTCGGCGTATAGcc               <  1:6397058/76‑1 (MQ=255)
                                                              gTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGcc               >  2:585948/1‑76 (MQ=255)
                                                               ttCTGCCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGccc              <  2:2021354/76‑1 (MQ=255)
                                                               ttCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGccc              <  2:4956591/76‑1 (MQ=255)
                                                               ttCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGccc              >  1:1223169/1‑76 (MQ=255)
                                                                 ctcccccTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCAt            <  2:6322251/73‑1 (MQ=255)
                                                                 cTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCAt            <  2:1000664/76‑1 (MQ=255)
                                                                   cccccTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTa          <  2:1179572/75‑1 (MQ=255)
                                                                   aCCCCTGCGTAGGCTTGATAAGACGTGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGCCGTATAGCCCATTa          >  2:7255531/1‑76 (MQ=37)
                                                                   aCCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTa          >  2:2796496/1‑76 (MQ=255)
                                                                   aCCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTa          <  2:455657/76‑1 (MQ=255)
                                                                     cccTGCGTAGGCGTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTAcc        <  1:5362363/76‑1 (MQ=255)
                                                                          cGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGcc   >  2:1556553/1‑76 (MQ=255)
                                                                          cGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGcc   >  2:4500647/1‑76 (MQ=255)
                                                                          cGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGcc   <  2:486544/76‑1 (MQ=255)
                                                                          cGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGcc   >  1:4746464/1‑76 (MQ=255)
                                                                           gTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCa  >  1:2097852/1‑76 (MQ=255)
                                                                           gTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCa  >  1:2275012/1‑76 (MQ=255)
                                                                           gTAGGCCTGATAAGACGCGCCAGCGGCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCa  >  1:7475365/1‑76 (MQ=255)
                                                                           |                                                                           
ATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCA  >  REL606/2374494‑2374644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.