| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | REL606 | = 2948453 | 198 (0.900) | 3 (0.010) | 3/150 | 21.6 | 1.3% | coding (1853/2145 nt) | sbm | methylmalonyl‑CoA mutase |
| ? | REL606 | 3953252 = | 262 (1.190) | coding (270/2547 nt) | cyaA | adenylate cyclase | |||||
| Rejected: Coverage evenness skew score above cutoff. | |||||||||||
| Rejected: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 263 total aligned reads displayed.
GCCTGATGAAACTCAACGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTG > REL606/3953177‑3953327 | gCCTGATGAAACTCAACGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGaaa < 1:2752735/76‑1 (MQ=255) ccTGATGAAACTCAACGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAg > 2:79393/1‑76 (MQ=255) ccTGATGAAACTCAACGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAg < 2:1792815/76‑1 (MQ=255) tgatgaAACTCAACGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGt > 1:6840150/1‑76 (MQ=255) tgatgaAACTCAACGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGt > 2:5136831/1‑76 (MQ=255) tgatgaAACTCAACGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGt > 1:460422/1‑76 (MQ=255) tgatgaAACTCAACGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGt < 1:2599660/76‑1 (MQ=255) gatgaAACTCAACGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTg > 1:1016499/1‑76 (MQ=255) gatgaAACTCAACGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTg > 1:1780243/1‑76 (MQ=255) atgaAACTCAACGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGa > 2:181354/1‑76 (MQ=255) atgaAACTCAACGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGa < 1:3763958/76‑1 (MQ=255) atgaAACTCAACGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGa < 2:2803413/76‑1 (MQ=255) atgaAACTCAACGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGa < 2:4068195/76‑1 (MQ=255) tgaAACTCAACGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAg < 2:1126343/76‑1 (MQ=255) aaCTCAACGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCtt > 1:876886/1‑76 (MQ=255) aaCTCAACGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCtt < 2:5324019/76‑1 (MQ=255) tcTCAACGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTc > 2:5753041/2‑76 (MQ=255) aCTCAACGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTc > 2:3144502/1‑76 (MQ=255) cgcAACGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTcc < 1:6248383/74‑1 (MQ=255) cTCAACGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGt < 1:5443668/68‑1 (MQ=255) cTCAACGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTcc < 2:1033778/76‑1 (MQ=255) tCAACGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCa > 1:6114477/1‑76 (MQ=255) ccAACGCCACTCCCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCa < 2:325758/75‑1 (MQ=255) caccGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCaa < 1:442735/73‑1 (MQ=255) cAACGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCaa > 2:6057140/1‑76 (MQ=255) cAACGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCaa < 1:6559570/76‑1 (MQ=255) aCGCCACTCCCGGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAAtt < 2:1916806/76‑1 (MQ=255) aCGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAAtt < 2:1940209/76‑1 (MQ=255) aCGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAAtt < 1:2188986/76‑1 (MQ=255) ccccTACCGGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACt < 2:4795068/73‑1 (MQ=255) ccACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACt > 2:4209312/1‑76 (MQ=255) ccACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACt > 1:1505425/1‑76 (MQ=255) cACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTg > 2:3193577/1‑76 (MQ=255) aCTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTgg > 2:6311685/1‑76 (MQ=255) aCTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTgg > 2:860003/1‑76 (MQ=255) tACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGgtg > 2:2340314/1‑76 (MQ=255) tACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGgtg < 1:2698850/76‑1 (MQ=255) tACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGgtg < 2:1618057/76‑1 (MQ=255) tACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGgtg > 2:4825558/1‑76 (MQ=255) tACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGgtg > 1:1606340/1‑76 (MQ=255) tACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGGGAGCTTCCAATTACTCgtg > 2:5586120/1‑76 (MQ=255) aaCGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATaca < 2:3553026/76‑1 (MQ=255) aaCGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATaca < 1:5902883/76‑1 (MQ=255) aaCGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATaca < 1:6145336/76‑1 (MQ=255) aggAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATacac < 2:2655720/74‑1 (MQ=255) aCGAGCTTGAACTGTATCGTGGCATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATccac > 2:574006/1‑76 (MQ=255) gAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATacacaa > 2:4266592/1‑74 (MQ=255) gAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCa < 1:5325998/76‑1 (MQ=255) gAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCa < 1:311242/76‑1 (MQ=255) aGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCAt < 1:81802/76‑1 (MQ=255) gCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATg < 1:3189168/76‑1 (MQ=255) gCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATg > 1:4114384/1‑76 (MQ=255) cTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATgg < 1:3459253/76‑1 (MQ=255) cTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATgg < 2:5842485/76‑1 (MQ=255) tGAACTGTATCGTGAAATGTGTGTACAGGACCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGgc < 2:5646679/76‑1 (MQ=255) aaCTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGgcag > 2:6449665/1‑76 (MQ=255) aaCTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGgcag < 2:6277871/76‑1 (MQ=255) aaCTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGgcag < 2:3785102/76‑1 (MQ=255) aCTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGgcagc > 2:4681059/1‑76 (MQ=255) aCTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGgcagc > 2:2170377/1‑76 (MQ=255) cTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGgcagca < 1:6774927/76‑1 (MQ=255) tGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCAc < 2:3900091/76‑1 (MQ=255) tATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCt > 2:2249698/1‑76 (MQ=255) tATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCt < 1:5555370/76‑1 (MQ=255) tATCGTGAAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCt < 1:2381117/76‑1 (MQ=255) aTCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTc > 1:4050980/1‑76 (MQ=255) tCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCg < 2:3397081/76‑1 (MQ=255) cGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGt > 1:5807706/1‑76 (MQ=255) gTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTc > 1:6731506/1‑76 (MQ=255) tGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTcc < 1:1199338/76‑1 (MQ=255) ggAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCg > 2:2504115/1‑76 (MQ=255) aTGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAg > 1:3398204/1‑76 (MQ=255) aTGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAg > 1:3900785/1‑76 (MQ=255) aTGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAg < 1:2500832/76‑1 (MQ=255) gTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAggg < 2:676940/76‑1 (MQ=255) tCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGc > 2:1472384/1‑76 (MQ=255) gTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCaaa > 1:1893298/1‑76 (MQ=255) tACAGGACCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAg < 1:202485/76‑1 (MQ=255) aCAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAgt > 1:728551/1‑76 (MQ=255) cAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAgtt < 1:1571529/76‑1 (MQ=255) aGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAgttg > 2:7007579/1‑76 (MQ=255) aGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGAATACA‑CATGGGCCGCCCCTCGTCCGTAGGGCCAAattgc > 2:209217/1‑75 (MQ=255) ggATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAgttgt < 1:5943943/76‑1 (MQ=255) ggATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCACCACCTCGTCCGTAGGGCAAAgttgt < 1:2884314/76‑1 (MQ=255) gATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAgttgtt < 1:5145042/76‑1 (MQ=255) aTCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTc > 2:1512746/1‑76 (MQ=255) aTCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTc > 1:6382405/1‑76 (MQ=255) tCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTcc > 2:6168137/1‑76 (MQ=255) tCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTcc > 2:3699365/1‑76 (MQ=255) ccgccgAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCct < 2:3962038/76‑1 (MQ=255) cgccgAAAGGGGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCctc < 2:5697720/76‑1 (MQ=255) ccgAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTg < 2:7063933/76‑1 (MQ=255) cgAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGa > 1:5646357/1‑76 (MQ=255) gAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGAc > 1:5237203/1‑76 (MQ=255) gAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGAc < 2:6597040/76‑1 (MQ=255) aaaGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGAcc < 2:5421525/76‑1 (MQ=255) aaGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCAGCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCt > 1:958224/1‑76 (MQ=255) aaGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCt > 1:215575/1‑76 (MQ=255) aaGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCt < 1:4494119/76‑1 (MQ=255) aaGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCt > 1:4253800/1‑76 (MQ=255) | GCCTGATGAAACTCAACGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTG > REL606/3953177‑3953327 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |