Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	2,448,493	IS186 (–) +6 bp	intergenic (+24/+21)	nupC → / ← yfeA	nucleoside (except guanosine) transporter/predicted diguanylate cyclase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	2448493 =	0 (0.000)	41 (1.130)	33/144	0.2	100%	intergenic (+24/+26)	nupC/yfeA	nucleoside (except guanosine) transporter/predicted diguanylate cyclase
	?	REL606	2772854 =	NA (NA)					noncoding (1/1343 nt)	IS186	repeat region
*	?	REL606	= 2448498	0 (0.000)	44 (1.210)	33/144	0.2	100%	intergenic (+29/+21)	nupC/yfeA	nucleoside (except guanosine) transporter/predicted diguanylate cyclase
	?	REL606	= 2774196	NA (NA)					noncoding (1343/1343 nt)	IS186	repeat region

CAAGGGTATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATccc‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/2448560‑2448493 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAA  >  REL606/2772854‑2772930                                                                                                                                                   CAAGGGTATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAG                                                                       <  1:172512/76‑1      GTATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTA                                                                  >  1:1056843/1‑76      GTATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTA                                                                  >  2:602357/1‑76       TATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAA                                                                 <  1:1010417/76‑1        ATTTCACTGGTCGCCCGCAGCCATTAGCTGATTAACAGTCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAAC                                                                >  1:69454/1‑76          TTGATTGGTCGCCCGCAGNCATTAGCTGATTAACAGGCGAAAAAAAGCCGGGGAATTATCCCATAAGCGCTAACTT                                                              >  1:1158402/1‑76          TTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTT                                                              <  1:810154/76‑1          TTGATTGGTCGCCAGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTT                                                              >  1:75845/1‑76            GATTGGTCGCCCGNAGCCATTAGCTGATNAANCGGCGTAAAAAAACCGTGGAATTATCCCATAAGCGCTAACTTAA                                                            <  2:1088958/76‑1            GATTGGTCGCCCGCAGCCATTAGCTGTTTAACAGGCGTAAAAAAACCGGGGTATGATACCATAAGCGCTAACTTAA                                                            >  1:1147510/1‑76              TTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCGATAAGCGCTCACTTAGGG                                                          >  2:49655/1‑76              TTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGG                                                          <  1:357144/76‑1              TTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGAGGAATTATCCCATAAGCGCTAACTTAAGG                                                          >  2:80253/1‑76                GGTCGCCCGCAGCCATTAGCTGATTAAAAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGT                                                        >  2:419168/1‑76                  TCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTG                                                      <  2:386543/76‑1                    GCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGTG                                                    <  1:396008/76‑1                         CAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATT                                               <  1:237875/76‑1                         CAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATT                                               <  1:274891/76‑1                          AGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTA                                              >  1:578361/1‑76                           GCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTAC                                             >  1:641104/1‑76                            CCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACG                                            >  2:143416/1‑76                             CATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGC                                           >  2:522154/1‑76                              ATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCC                                          >  1:841139/1‑76                               TTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCT                                         <  1:663426/76‑1                                         AACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTTAGGGTTGTGGTATTACGCCTGATATGATTT                               <  1:1109425/76‑1                                              GCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGT                          <  2:974730/76‑1                                               CGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTG                         >  2:310367/1‑76                                                GTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGC                        >  2:990345/1‑76                                                GTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGC                        <  1:984115/76‑1                                                  AAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCG                      >  1:903096/1‑76                                                    AAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGAT                    <  2:412487/76‑1                                                         CCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATT               <  1:594658/76‑1                                                          CGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTA              >  1:746433/1‑76                                                           gatGAATTATCCCATAATCGCTAACCTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAC             <  2:426655/73‑1                                                             GGAATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTC           <  2:867509/76‑1                                                                ATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCA        <  1:886558/76‑1                                                                 TTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCAC       >  1:549264/1‑76                                                                  TATCCCATAAGCGCTAAGTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACATGCCGACGAATTACTCTCACG      >  2:184514/1‑76                                                                   ATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGA     >  1:204807/1‑76                                                                   ATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGA     >  1:477706/1‑76                                                                    aCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGAT    <  2:367275/75‑1                                                                    aCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGAT    <  1:888664/75‑1                                                                    TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGAT    >  2:253125/1‑76                                                                     CCCATAAGCGCTAGCTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATA   >  1:77045/1‑76                                                                     CCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATA   <  1:704657/76‑1                                                                     CCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATA   <  2:413895/76‑1                                                                     CCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATA   <  1:134441/76‑1                                                                     CCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATA   <  1:724028/76‑1                                                                     CCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATA   <  1:518120/76‑1                                                                     CCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATA   <  2:1120773/76‑1                                                                      CCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAA  <  2:650889/76‑1                                                                      CCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAA  >  1:394065/1‑76                                                                      CCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGACGAATTACTCTCACGATca  >  2:523349/1‑74                                                                                                                                                   CAAGGGTATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATccc‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/2448560‑2448493 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAA  >  REL606/2772854‑2772930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.