Predicted mutation
evidence seq id position mutation annotation gene description
MC JC REL606 3,894,997 Δ6,934 bp IS150‑mediated rbsD–[yieO] rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ REL606 3893611–3894994 3901930 6937–8320 21 [20] [0] 70 [insJ‑5]–[yieO] [insJ‑5],insK‑5,rbsD,rbsA,rbsC,rbsB,rbsK,rbsR,[yieO]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 = 3652533NA (NA)67 (1.160) 51/146 0.1 100% noncoding (1443/1443 nt) IS150 repeat region
?REL606 3901931 = 0 (0.000)coding (489/1428 nt) yieO predicted multidrug or homocysteine efflux system

TCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGA  >  REL606/3893535‑3893626
                                                                           |                
tCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAACCCCTAATATGTCAAAGc                  >  1:597650/1‑76 (MQ=255)
 cGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGcc                 <  1:1056890/76‑1 (MQ=255)
 cGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGcc                 <  2:893054/76‑1 (MQ=255)
   aaaTCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCaa               <  1:1644155/76‑1 (MQ=255)
   aaaTCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCaa               <  1:309/76‑1 (MQ=255)
      tCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAAt            >  1:61173/1‑76 (MQ=255)
      tCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAAt            >  1:1409925/1‑76 (MQ=255)
        tAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATAc          >  2:40230/1‑76 (MQ=255)
        tAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATAc          <  1:482745/76‑1 (MQ=255)
         aaCGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATAcc         >  1:1004515/1‑76 (MQ=255)
             ccAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCttt     <  1:45880/76‑1 (MQ=38)
             ccAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCttt     >  1:657036/1‑76 (MQ=38)
             ccAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCttt     >  2:1349080/1‑76 (MQ=38)
             ccAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCttt     <  2:346834/76‑1 (MQ=38)
             ccAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCttt     <  1:338153/76‑1 (MQ=38)
              cAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCtttt    <  2:1685311/76‑1 (MQ=35)
                gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa  >  1:687496/1‑76 (MQ=32)
                gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa  >  1:787237/1‑76 (MQ=32)
                gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa  >  2:144804/1‑76 (MQ=32)
                gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa  <  2:61173/76‑1 (MQ=32)
                aaCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa  >  1:154013/2‑76 (MQ=14)
                                                                           |                
TCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGA  >  REL606/3893535‑3893626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑