Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	2,448,493	IS186 (+) +6 bp	intergenic (+24/+21)	nupC → / ← yfeA	nucleoside (except guanosine) transporter/predicted diguanylate cyclase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	2448493 =	0 (0.000)	68 (1.290)	48/144	0.1	100%	intergenic (+24/+26)	nupC/yfeA	nucleoside (except guanosine) transporter/predicted diguanylate cyclase
	?	REL606	= 2774196	NA (NA)					noncoding (1343/1343 nt)	IS186	repeat region
*	?	REL606	= 2448498	1 (0.020)	66 (1.250)	48/144	0.1	98.4%	intergenic (+29/+21)	nupC/yfeA	nucleoside (except guanosine) transporter/predicted diguanylate cyclase
	?	REL606	2772854 =	NA (NA)					noncoding (1/1343 nt)	IS186	repeat region

ATATCTGCAAGGGTATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATccc‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/2448567‑2448493 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGT  <  REL606/2774196‑2774119                                                                                                                                                           ATATCTGCAAGGGTATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATC                                                                               >  1:850753/1‑76  TATCTGCAAGGGTATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCC                                                                              >  1:1616204/1‑76  TATCTGCAAGGGTATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCC                                                                              >  2:995786/1‑76   ATCTGCAAGGGTATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCC                                                                             <  2:627630/76‑1   ATCTGCAAGGGTATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCC                                                                             <  1:674069/76‑1   ATCTGCAAGGGTATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCC                                                                             >  1:1451445/1‑76   ATCTGCAAGGGTATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCC                                                                             >  2:356891/1‑76    TCTGCAAGGGTATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCA                                                                            <  1:453162/76‑1    TCTGCAAGGGTATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCA                                                                            >  2:1719423/1‑76       GCAAGGGTATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAA                                                                         <  2:711167/76‑1       GCAAGGGTATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAA                                                                         >  1:430110/1‑76       GCAAGGGTATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAA                                                                         <  2:1468800/76‑1        CAAGGGTATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAGTTATCCCATCAG                                                                        >  2:377484/1‑76        CAAGGGTATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAG                                                                        >  1:886540/1‑76        CAAGGGTATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAG                                                                        <  1:1540723/76‑1        CAAGGGTATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAG                                                                        >  2:1551198/1‑76        CAAGGGTATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAG                                                                        >  2:1038138/1‑76         AAGGGTATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGC                                                                       <  2:1403923/76‑1          AGGGTATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCG                                                                      <  2:1279163/76‑1            GGTATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCT                                                                    <  1:774459/76‑1               ATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAAC                                                                 >  2:202449/1‑76               ATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAAC                                                                 >  1:1244591/1‑76                TTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACT                                                                <  1:1618680/76‑1                TTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACT                                                                >  1:1436224/1‑76                 TTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTT                                                               >  2:200887/1‑76                    ATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAG                                                            <  1:813378/76‑1                     TTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGG                                                           <  1:823492/76‑1                      TGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGG                                                          >  1:869018/1‑76                      TGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGG                                                          >  1:1712108/1‑76                       GGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGT                                                         >  1:312025/1‑76                        GTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTT                                                        <  1:996263/76‑1                         TCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTG                                                       <  1:1519940/76‑1                         TCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTG                                                       >  1:898505/1‑76                         TCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTG                                                       <  2:22545/76‑1                         TCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTG                                                       <  1:196575/76‑1                           GCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGAA                                                     <  2:409825/76‑1                               GCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGAACCAT                                                 <  1:1647370/76‑1                                CAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGAACCATC                                                >  1:713782/1‑76                                CAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGAACCATC                                                >  1:1293985/1‑76                                CAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGAACCATC                                                >  1:232763/1‑76                                   CCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGA                                             <  2:706874/76‑1                                    CATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAA                                            >  2:1467383/1‑76                                    CATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAA                                            <  2:1047261/76‑1                                    CATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAA                                            <  2:953101/76‑1                                      TTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGA                                          <  1:1443672/76‑1                                        AGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAAT                                        >  2:999429/1‑76                                        AGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAAT                                        >  1:1559938/1‑76                                          CTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGC                                      >  1:889565/1‑76                                           TGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCG                                     <  1:1493839/76‑1                                           TGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCG                                     >  1:1173229/1‑76                                            GATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGA                                    >  1:974955/1‑76                                                 ACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCT                               >  1:655408/1‑76                                                  CAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTC                              <  1:632149/76‑1                                                   AGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCG                             <  2:809526/76‑1                                                    GGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGG                            <  1:1296236/76‑1                                                         AAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCT                       >  2:549892/1‑76                                                         AAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCT                       <  2:734597/76‑1                                                          AAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTC                      >  1:938152/1‑76                                                            AAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGT                    >  2:465238/1‑76                                                              AACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTA                  <  2:1602163/76‑1                                                                CCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCTACGCCTCGGTGCCTCGTTAAG                <  2:1720028/76‑1                                                                CCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAG                <  1:868449/76‑1                                                                CCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAG                >  1:1591336/1‑76                                                                 CGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGA               >  2:851469/1‑76                                                                 CGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGA               >  1:682308/1‑76                                                                   GGGAATTATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACG             <  1:1444115/76‑1                                                                    GGAATTATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGA            >  1:671140/1‑76                                                                      AATTATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATG          >  1:435841/1‑76                                                                        TTATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCC        >  2:1342489/1‑76                                                                        TTATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCC        >  2:409805/1‑76                                                                          ATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTC      <  1:1045840/76‑1                                                                          ATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTC      <  1:1010225/76‑1                                                                          ATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCGCGGTGCCTCGTTAAGACGATGCCTC      >  1:508819/1‑76                                                                           gCCCATAAGCGCTAACTTAAGGGTTGAGCCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCG     >  2:1370655/2‑76                                                                           TCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCG     <  1:914493/76‑1                                                                           TCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCG     >  2:855205/1‑76                                                                            CCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGC    <  1:609776/76‑1                                                                            CCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGC    >  1:1451925/1‑76                                                                            CCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGC    <  1:813381/76‑1                                                                            CCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGC    <  1:214615/76‑1                                                                            CCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGC    >  1:206310/1‑76                                                                             CCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCG   >  2:368790/1‑76                                                                             CCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCG   <  2:1507865/76‑1                                                                             CCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCG   <  1:271351/76‑1                                                                             CCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCG   <  2:1054253/76‑1                                                                              CATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGT  >  1:28331/1‑76                                                                              CATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGT  <  2:102874/76‑1                                                                              CATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGT  >  1:248542/1‑76                                                                              CATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGT  >  1:334669/1‑76                                                                              CATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGT  >  1:363723/1‑76                                                                              CATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGT  <  1:894600/76‑1                                                                              CATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGT  >  2:1736952/1‑76                                                                                                                                                           ATATCTGCAAGGGTATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATccc‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/2448567‑2448493 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGT  <  REL606/2774196‑2774119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.