Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	REL606	1,305,725	Δ275 bp	coding (241‑515/1461 nt)	cls ←	cardiolipin synthetase

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	REL606	1305725	1305999	275	53 [0]	[1] 53	cls	cardiolipin synthetase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 1305724	0 (0.000)	52 (0.950)	39/150	0.4	99.1%	coding (516/1461 nt)	cls	cardiolipin synthetase
	?	REL606	1306000 =	1 (0.020)					coding (240/1461 nt)	cls	cardiolipin synthetase

ACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCGGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAACAC  >  REL606/1305649‑1305798                                                                            |                                                                           aCGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCaatgaatg                                                                            <  2:570679/76‑1 (MQ=255)    cATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGttt                                                                         <  2:1409099‑M1/76‑4 (MQ=255)    cATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGttt                                                                         <  1:402234‑M1/76‑4 (MQ=255)     aTTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGtttt                                                                        <  2:899528‑M1/76‑5 (MQ=255)     aTTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGtttt                                                                        <  1:813346‑M1/76‑5 (MQ=255)        aGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGttttgcg                                                                     >  1:500390‑M1/1‑69 (MQ=255)            cGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGttttgcggtgg                                                                 <  2:159201‑M1/76‑12 (MQ=255)             gggCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGttttgcggtgga                                                                >  1:78362‑M1/1‑64 (MQ=255)             gggCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGttttgcggtgga                                                                >  1:1402292‑M1/1‑64 (MQ=255)              ggCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGttttgcggtggaa                                                               <  2:1240797‑M1/76‑14 (MQ=255)                ccACGGGCTGCGGAAAAAAGCCGCACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGttttgcggtggaagg                                                             <  1:1043522‑M1/76‑16 (MQ=255)                 cACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGttttgcggtggaaggc                                                            >  2:1584852‑M1/1‑60 (MQ=255)                 cACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGttttgcggtggaaggc                                                            <  1:1505881‑M1/76‑17 (MQ=255)                      gCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGttttgcggtggaaggccacat                                                       <  2:769827‑M1/76‑22 (MQ=255)                           ggAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGttttgcggtggaaggccacatcgctc                                                  >  1:672281‑M1/1‑50 (MQ=255)                           ggAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGttttgcggtggaaggccacatcgctc                                                  >  1:772849‑M1/1‑50 (MQ=255)                               aaaaGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGttttgcggtggaaggccacatcgctctggc                                              <  1:932916‑M1/76‑31 (MQ=255)                               aaaaGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGttttgcggtggaaggccacatcgctctggc                                              <  2:1101387‑M1/76‑31 (MQ=255)                                aaaGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGttttgcggtggaaggccacatcgctctggcg                                             >  2:671364‑M1/1‑45 (MQ=255)                                aaaGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGttttgcggtggaaggccacatcgctctggcg                                             <  1:1062862‑M1/76‑32 (MQ=255)                                aaaGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGttttgcggtggaaggccacatcgctctggcg                                             >  2:763514‑M1/1‑45 (MQ=255)                                 aaGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGttttgcggtggaaggccacatcgctctggcgc                                            >  2:222059‑M1/1‑44 (MQ=255)                                    ccACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGttttgcggtggaaggccacatcgctctggcgcgct                                         >  2:338879‑M1/1‑41 (MQ=255)                                      acacTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGttttgcggtggaaggccacatcgctctggcgcgctca                                       <  2:801500‑M1/76‑38 (MQ=255)                                      acacTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGttttgcggtggaaggccacatcgctctggcgcgctca                                       <  1:768276‑M1/76‑38 (MQ=255)                                        acTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGttttgcggtggaaggccacatcgctctggcgcgctcagc                                     <  1:1696071‑M1/76‑40 (MQ=255)                                         cTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGttttgcggtggaaggccacatcgctctggcgcgctcagcg                                    >  1:938955‑M1/1‑36 (MQ=255)                                             cggcggAGTCGAGCATCAATCGGCAATGAATGttttgcggtggaaggccacatcgctctggcgcgctcagcgcggc                                <  1:339756‑M1/76‑45 (MQ=255)                                             cggcggAGTCGAGCATCAATCGGCAATGAATGttttgcggtggaaggccacatcgctctggcgcgctcagcgcggc                                <  2:540032‑M1/76‑45 (MQ=255)                                                 ggAGTCGAGCATCAATCGGCAATGAATGtcttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgttt                            <  2:296131‑M1/76‑49 (MQ=255)                                                   aGTCGAGCATCAATCGGCAATGAATGttttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgc                          <  2:934040‑M1/76‑51 (MQ=255)                                                   aGTCGAGCATCAATCGGCAATGAATGttttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgc                          <  2:76861‑M1/76‑51 (MQ=255)                                                   aGTCGAGCATCAATCGGCAATGAATGttttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgc                          >  2:786895‑M1/1‑26 (MQ=255)                                                   aGTCGAGCATCAATCGGCAATGAATGttttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgattgg                          >  2:786871‑M1/1‑26 (MQ=255)                                                           aTCAATCGGCAATGAATGttttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaatgg                  >  1:106343‑M1/1‑18 (MQ=255)                                                             cAATCGGCAATGAATGttttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaatggag                <  2:1451293‑M1/76‑61 (MQ=255)                                                              aaTCGGCAATGAATGttttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaatggagc               <  2:625619‑M1/76‑62 (MQ=255)                                                               aTCGGCAATGAATGttttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaatggagct              >  2:967009‑M1/1‑14 (MQ=255)                                                               aTCGGCAATGAATGttttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaatggagct              <  2:119808‑M1/76‑63 (MQ=255)                                                                tCGGCAATGAATGttttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaatggagctc             <  2:53341‑M1/76‑64 (MQ=255)                                                                tCGGCAATGAATGttttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaatggagctc             <  2:1232250‑M1/76‑64 (MQ=255)                                                                tCGGCAATGAATGttttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaatggagctc             <  1:1216996‑M1/76‑64 (MQ=255)                                                                tCGGCAATGAATGttttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaatggagctc             <  2:881171‑M1/76‑64 (MQ=255)                                                                 cGGCAATGAATGttttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaatggagctcg            >  1:1281639‑M1/1‑12 (MQ=255)                                                                  ggCAATGAATGttttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaatggagctcgc           >  1:537577‑M1/1‑11 (MQ=255)                                                                    cAATGAATGttttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaatggagctcgcca         <  2:345219‑M1/76‑68 (MQ=255)                                                                    cAATGAATGttttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaatggagctcgcca         >  2:144685‑M1/1‑9 (MQ=255)                                                                     aatgaatgttttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaatggagctcgccaa        >  2:1275645‑M1/1‑8 (MQ=255)                                                                      atgaatgttttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaatggagctcgccaac       <  1:1209542‑M1/76‑70 (MQ=255)                                                                        gaatgttttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaatggagctcgccaacgg     >  1:1311624‑M1/1‑5 (MQ=255)                                                                          atgttttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaatggagctcgccaacggca   >  2:474322‑M1/1‑3 (MQ=255)                                                                          atgttttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaatggagctcgccaacggca   <  1:1104508‑M1/76‑74 (MQ=255)                                                                           tgttttgcggtggaaggccacatcgctctggcgcgctcagcgcggcgtttgcctaaatggagctcgccaacggcaa  >  1:1049397‑M1/1‑2 (MQ=255)                                                                            |                                                                           ACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCGGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAACAC  >  REL606/1305649‑1305798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑