Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	REL606	1,305,725	Δ275 bp	coding (241‑515/1461 nt)	cls ←	cardiolipin synthetase

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	REL606	1305725	1305999	275	53 [0]	[1] 53	cls	cardiolipin synthetase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 1305724	0 (0.000)	52 (0.950)	39/150	0.4	99.1%	coding (516/1461 nt)	cls	cardiolipin synthetase
	?	REL606	1306000 =	1 (0.020)					coding (240/1461 nt)	cls	cardiolipin synthetase

TGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAA  >  REL606/1305927‑1306073                                                                          |                                                                          cattagctcgggccacgggctgcggaaaaaagccacactcccggcggagtcgagcatcaatcggcaatgaatgttt                                                                         <  2:1409099‑M2/3‑1 (MQ=255) cattagctcgggccacgggctgcggaaaaaagccacactcccggcggagtcgagcatcaatcggcaatgaatgttt                                                                         <  1:402234‑M2/3‑1 (MQ=255)  attagctcgggccacgggctgcggaaaaaagccacactcccggcggagtcgagcatcaatcggcaatgaatgtttt                                                                        <  1:813346‑M2/4‑1 (MQ=255)  attagctcgggccacgggctgcggaaaaaagccacactcccggcggagtcgagcatcaatcggcaatgaatgtttt                                                                        <  2:899528‑M2/4‑1 (MQ=255)     agctcgggccacgggctgcggaaaaaagccacactcccggcggagtcgagcatcaatcggcaatgaatgTTTTGCg                                                                     >  1:500390‑M2/70‑76 (MQ=255)         cgggccacgggctgcggaaaaaagccacactcccggcggagtcgagcatcaatcggcaatgaatgTTTTGCGGTgg                                                                 <  2:159201‑M2/11‑1 (MQ=255)          gggccacgggctgcggaaaaaagccacactcccggcggagtcgagcatcaatcggcaatgaatgTTTTGCGGTGGa                                                                >  1:78362‑M2/65‑76 (MQ=255)          gggccacgggctgcggaaaaaagccacactcccggcggagtcgagcatcaatcggcaatgaatgTTTTGCGGTGGa                                                                >  1:1402292‑M2/65‑76 (MQ=255)           ggccacgggctgcggaaaaaagccacactcccggcggagtcgagcatcaatcggcaatgaatgTTTTGCGGTGGaa                                                               <  2:1240797‑M2/13‑1 (MQ=255)             ccacgggctgcggaaaaaagccgcactcccggcggagtcgagcatcaatcggcaatgaatgTTTTGCGGTGGAAgg                                                             <  1:1043522‑M2/15‑1 (MQ=255)              cacgggctgcggaaaaaagccacactcccggcggagtcgagcatcaatcggcaatgaatgTTTTGCGGTGGAAGGc                                                            <  1:1505881‑M2/16‑1 (MQ=255)              cacgggctgcggaaaaaagccacactcccggcggagtcgagcatcaatcggcaatgaatgTTTTGCGGTGGAAGGc                                                            >  2:1584852‑M2/61‑76 (MQ=255)                   gctgcggaaaaaagccacactcccggcggagtcgagcatcaatcggcaatgaatgTTTTGCGGTGGAAGGCCACAt                                                       <  2:769827‑M2/21‑1 (MQ=255)                        ggaaaaaagccacactcccggcggagtcgagcatcaatcggcaatgaatgTTTTGCGGTGGAAGGCCACATCGctc                                                  >  1:672281‑M2/51‑76 (MQ=255)                        ggaaaaaagccacactcccggcggagtcgagcatcaatcggcaatgaatgTTTTGCGGTGGAAGGCCACATCGctc                                                  >  1:772849‑M2/51‑76 (MQ=255)                            aaaagccacactcccggcggagtcgagcatcaatcggcaatgaatgTTTTGCGGTGGAAGGCCACATCGCTCTGgc                                              <  2:1101387‑M2/30‑1 (MQ=255)                            aaaagccacactcccggcggagtcgagcatcaatcggcaatgaatgTTTTGCGGTGGAAGGCCACATCGCTCTGgc                                              <  1:932916‑M2/30‑1 (MQ=255)                             aaagccacactcccggcggagtcgagcatcaatcggcaatgaatgTTTTGCGGTGGAAGGCCACATCGCTCTGgcg                                             >  2:671364‑M2/46‑76 (MQ=255)                             aaagccacactcccggcggagtcgagcatcaatcggcaatgaatgTTTTGCGGTGGAAGGCCACATCGCTCTGgcg                                             >  2:763514‑M2/46‑76 (MQ=255)                             aaagccacactcccggcggagtcgagcatcaatcggcaatgaatgTTTTGCGGTGGAAGGCCACATCGCTCTGgcg                                             <  1:1062862‑M2/31‑1 (MQ=255)                              aagccacactcccggcggagtcgagcatcaatcggcaatgaatgTTTTGCGGTGGAAGGCCACATCGCTCTGgcgc                                            >  2:222059‑M2/45‑76 (MQ=255)                                 ccacactcccggcggagtcgagcatcaatcggcaatgaatgTTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCt                                         >  2:338879‑M2/42‑76 (MQ=255)                                   acactcccggcggagtcgagcatcaatcggcaatgaatgTTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCa                                       <  2:801500‑M2/37‑1 (MQ=255)                                   acactcccggcggagtcgagcatcaatcggcaatgaatgTTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCa                                       <  1:768276‑M2/37‑1 (MQ=255)                                     actcccggcggagtcgagcatcaatcggcaatgaatgTTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAgc                                     <  1:1696071‑M2/39‑1 (MQ=255)                                      ctcccggcggagtcgagcatcaatcggcaatgaatgTTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAgcg                                    >  1:938955‑M2/37‑76 (MQ=255)                                          cggcggagtcgagcatcaatcggcaatgaatgTTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCgcggc                                <  2:540032‑M2/44‑1 (MQ=255)                                          cggcggagtcgagcatcaatcggcaatgaatgTTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCgcggc                                <  1:339756‑M2/44‑1 (MQ=255)                                              ggagtcgagcatcaatcggcaatgaatgTCTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGttt                            <  2:296131‑M2/48‑1 (MQ=255)                                                agtcgagcatcaatcggcaatgaatgTTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGc                          <  2:76861‑M2/50‑1 (MQ=255)                                                agtcgagcatcaatcggcaatgaatgTTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGc                          <  2:934040‑M2/50‑1 (MQ=255)                                                agtcgagcatcaatcggcaatgaatgTTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGc                          >  2:786895‑M2/27‑76 (MQ=255)                                                agtcgagcatcaatcggcaatgaatgTTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGATtgg                          >  2:786871‑M2/27‑75 (MQ=255)                                                        atcaatcggcaatgaatgTTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATgg                  >  1:106343‑M2/19‑76 (MQ=255)                                                         ttAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTCAATGGa                 >  2:1612322/1‑76 (MQ=255)                                                          caatcggcaatgaatgTTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAg                <  2:1451293‑M2/60‑1 (MQ=255)                                                           aatcggcaatgaatgTTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGc               <  2:625619‑M2/61‑1 (MQ=255)                                                            atcggcaatgaatgTTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCt              >  2:967009‑M2/15‑76 (MQ=255)                                                            atcggcaatgaatgTTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCt              <  2:119808‑M2/62‑1 (MQ=255)                                                             tcggcaatgaatgTTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTc             <  2:53341‑M2/63‑1 (MQ=255)                                                             tcggcaatgaatgTTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTc             <  2:1232250‑M2/63‑1 (MQ=255)                                                             tcggcaatgaatgTTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTc             <  1:1216996‑M2/63‑1 (MQ=255)                                                             tcggcaatgaatgTTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTc             <  2:881171‑M2/63‑1 (MQ=255)                                                              cggcaatgaatgTTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCg            >  1:1281639‑M2/13‑76 (MQ=255)                                                               ggcaatgaatgTTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGc           >  1:537577‑M2/12‑76 (MQ=255)                                                                 caatgaatgTTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCa         <  2:345219‑M2/67‑1 (MQ=255)                                                                 caatgaatgTTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCa         >  2:144685‑M2/10‑76 (MQ=255)                                                                  aatgaatgTTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCaa        >  2:1275645‑M2/9‑76 (MQ=255)                                                                   atgaatgTTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAAc       <  1:1209542‑M2/69‑1 (MQ=255)                                                                     gaatgTTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACgg     >  1:1311624‑M2/6‑76 (MQ=255)                                                                       atgTTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCa   >  2:474322‑M2/4‑76 (MQ=255)                                                                       atgTTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCa   <  1:1104508‑M2/73‑1 (MQ=255)                                                                        tgTTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCaa  >  1:1049397‑M2/3‑76 (MQ=255)                                                                          |                                                                          TGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAA  >  REL606/1305927‑1306073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑