BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	REL606	1,303,175	Δ1,633 bp		[oppD]–[cls]	[oppD], oppF, yciU, [cls]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	REL606	1303175	1304807	1633	50 [2]	[0] 48	[oppD]–[cls]	[oppD],oppF,yciU,[cls]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 1303174	2 (0.030)	47 (0.810)	35/148	0.5	97.9%	coding (827/1014 nt)	oppD	oligopeptide transporter ATP‑binding component
*	?	REL606	1304808 =	0 (0.000)	47 (0.810)	35/148	0.5	97.9%	coding (1432/1461 nt)	cls	cardiolipin synthetase

TATCCATAGTCACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAACGGGCTGAAGAAGTAAAACAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATAT  >  REL606/1304738‑1304882

cgcgcgatgtcttttatcaacccgttcatccttattctatcggtttgctcaacgcggtgccgcgtcttgaGTCGCt                                                                       <  2:1545194‑M2/6‑1 (MQ=255)

 gcgcgatgtcttttatcaacccgttcatccttattctatcggtttgctcaacgcggtgccgcgtcttgaGTCGCTc                                                                      <  2:838380‑M2/7‑1 (MQ=255)

  cgcgatgtcttttatcaacccgttcatccttattctatcggtttgctcaacgcggtgccgcgtcttgaGTCGCTCg                                                                     >  1:1023956‑M2/69‑76 (MQ=255)

      atgtcttttatcaacccgttcatccttattctatcggtttgctcaacgcggtgccgcgtcttgaGTCGCTCGGCGa                                                                 >  1:743682‑M2/65‑76 (MQ=255)

        gtcttttatcaacccgttcatccttattctatcggtttgctcaacgcggtgccgcgtcttgaGTCGCTCGGCgacc                                                               >  2:762‑M2/63‑75 (MQ=255)

          cttttatcaacccgttcatccttattctatcggtttgctcaacgcggtgccgcgtcttgaGTCGCTCGGCGACACg                                                             >  1:933843‑M2/61‑76 (MQ=255)

             ttatcaacccgttcatccttattctatcggtttgctcaacgcggtgccgcgtcttgaGTCGCTCGGCGACACGTTg                                                          >  1:84068‑M2/58‑76 (MQ=255)

                  aacccgttcatccttattctatcggtttgctcaacgcggtgccgcgtcttgaGTCGCTCGGCGACACGTTGCCATa                                                     >  2:101259‑M2/53‑76 (MQ=255)

                    cccgttcatcctttttctatcggtttgctcaacgcggtgccgcgtcttgaGTCGCTCGGCGACACGTTGCCATAAt                                                   <  2:1250875‑M2/26‑1 (MQ=255)

                     ccgttcatccttattctatcggtttgctcaacgcggtgccgcgtcttgaGTCGCTCGGCGACACGTTGCCATAATg                                                  >  2:869337‑M2/50‑76 (MQ=255)

                         tcatccttattctatcggtttgctcaacgcggtgccgcgtcttgaGTCGCTCGGCGACACGTTGCCATAATGGACg                                              <  2:1812427‑M2/31‑1 (MQ=255)

                             ccttattctatcggtttgctcaacgcggtgccgcgtcttgaGTCGCTCGGCGACACGTTGCCATAATGGACGtttt                                          <  2:1793149‑M2/35‑1 (MQ=255)

                             ccttattctatcggtttgctcaacgcggtgccgcgtcttgaGTCGCTCGGCGACACGTTGCCATAATGGACGtttt                                          >  1:490175‑M2/42‑76 (MQ=255)

                             ccttattctatcggtttgctcaacgcggtgccgcgtcttgaGTCGCTCGGCGACACGTTGCCATAATGGACGtttt                                          <  2:700734‑M2/35‑1 (MQ=255)

                              cttattctatcggtttgctcaccgcggtgccgcgtcttgaGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTa                                         <  1:572278‑M2/36‑1 (MQ=255)

                              cttattctatcggtttgctcaacgcggtgccgcgtcttgaGTCGCTCGGCGACCGGTTGCCATTATGGAGGTTTTa                                         >  1:561769‑M2/41‑76 (MQ=255)

                              cttattctatcggtttgctcaacgcggtgccgcgtcttgaGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTa                                         >  1:561757‑M2/41‑76 (MQ=255)

                              cttattctatcggtttgctcaacgcggtgccgcgtcttgaGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTa                                         <  2:512662‑M2/36‑1 (MQ=255)

                                tattctatcggtttgctcaacgcggtgccgcgtcttgaGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGc                                       >  1:1828511‑M2/39‑76 (MQ=255)

                                 attctatcggtttgctcaacgcggtgccgcgtcttgaGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGcc                                      >  1:1082874‑M2/38‑76 (MQ=255)

                                 attctatcggtttgctcaacgcggtgccgcgtcttgaGTCGCTCGGCGACACGTTGCCATAACGGACGTTTTAGcc                                      >  2:818950‑M2/38‑76 (MQ=255)

                                     tatcggtttgctcaacgcggtgccgcgtcttgaGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATaa                                  >  2:1208585‑M2/34‑76 (MQ=255)

                                      atcggtttgctcaacgcggtgccgcgtcttgaGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATaaa                                 <  2:1441652‑M2/44‑1 (MQ=255)

                                        cggtttgctcaacgcggtgccgcgtcttgaGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACg                               >  1:1287256‑M2/31‑76 (MQ=255)

                                          gtttgctcaacgcggtgccgcgtcttgaGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACggg                             <  1:1208585‑M2/48‑1 (MQ=255)

                                           tttgctcaacgcggtgccgcgtcttgaGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGc                            >  1:763423‑M2/28‑76 (MQ=255)

                                             tgctcaacgcggtgccgcgtcttgaGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCAt                          >  1:15622‑M2/26‑76 (MQ=255)

                                              gctcagcgcggtgccgcgtcttgaGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATc                         <  2:1431862‑M2/52‑1 (MQ=255)

                                              gctcaacgcggtgccgcgtcttgaGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATc                         >  1:845438‑M2/25‑76 (MQ=255)

                                              gctcaacgcggtgccgcgtcttgaGTCGCTCGGCGACACGTTGCCATAATGGACGCTTTAGCCATAAACGCGCCTc                         >  2:760176‑M2/25‑76 (MQ=255)

                                               ctcaacgcggtgccgcgtcttgaGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCg                        <  2:1508132‑M2/53‑1 (MQ=255)

                                               ctcaacgcggtgccgcgtcttgaGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCg                        >  2:750834‑M2/24‑76 (MQ=255)

                                               ctcaacgcggtgccgcgtcttgaGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCg                        >  2:252526‑M2/24‑76 (MQ=255)

                                                  aacgcggtgccgcgtcttgaGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGc                     >  2:1744327‑M2/21‑76 (MQ=255)

                                                  aacgcggtgccgcgtcttgaGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGc                     >  2:12563‑M2/21‑76 (MQ=255)

                                                     ggggtgccgcgtcttggGTCCCTCGGCGACCCGTTGCCATAATGGACTTTTTAGCCATAAACGGGCATCGAGCAGa                  <  1:402407‑M2/59‑1 (MQ=255)

                                                      cggtgccgcgtcttgaGTCGCTCGGCGACACGTTGCCATAATGGACTTTTTAGCCATAAACGGGCATCGAGCAGAc                 <  2:1561729‑M2/60‑1 (MQ=255)

                                                      cggtgccgcgtcttgaGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGAc                 <  2:1192597‑M2/60‑1 (MQ=255)

                                                            cgcgtcttgaGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAAc           <  1:1525704‑M2/66‑1 (MQ=255)

                                                            cgcgtcttgaGCCGCTGGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAAc           <  1:270609‑M2/66‑1 (MQ=255)

                                                             gcgtcttgaGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAAcg          <  2:977466‑M2/67‑1 (MQ=255)

                                                             gcgtcttgaGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAAcg          <  2:259069‑M2/67‑1 (MQ=255)

                                                             gcgtcttgaGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAAcg          <  1:1481794‑M2/67‑1 (MQ=255)

                                                             gcgtcttgaGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAAcg          <  1:1112983‑M2/67‑1 (MQ=255)

                                                                 cttgaGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGaa      <  2:609164‑M2/71‑1 (MQ=255)

                                                                 cttgaGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGaa      >  1:165577‑M2/6‑76 (MQ=255)

                                                                   tgaGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAat    >  1:819165‑M2/4‑76 (MQ=255)

                                                                     aGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAatat  >  2:1202780‑M2/2‑76 (MQ=255)

TATCCATAGTCACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAACGGGCTGAAGAAGTAAAACAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATAT  >  REL606/1304738‑1304882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`