Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	REL606	2,563,623	+GTAG	coding (41/1014 nt)	yfgA ←	hypothetical protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 2563618	1 (0.020)	56 (0.900) +GGTA	41/132	0.3	98.3%	coding (46/1014 nt)	yfgA	hypothetical protein
	?	REL606	2563619 =	1 (0.020)					coding (45/1014 nt)	yfgA	hypothetical protein

TTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/2563545‑2563618 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGA  >  REL606/2563619‑2563693                                                                           ||||                                                                            TTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGG                                                                               >  1:968586/1‑76  TCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGT                                                                              <  2:1086989/76‑1  TCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGT                                                                              <  2:1299965/76‑1  TCAGGCAAAGCCGCGCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGT                                                                              <  2:548441/76‑1   CAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTA                                                                             <  1:1860198/76‑1    AGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAG                                                                            <  2:500996/76‑1    AGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAG                                                                            >  2:1172512/1‑76       CAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGGTA                                                                         >  2:1206069/1‑76          AGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGGTAGTA                                                                      >  2:313762/1‑76          AGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGGTAGTA                                                                      >  2:1337935/1‑76             CGCTCGGCAACGGTCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGGGAGTAAGT                                                                   >  2:571503/1‑76             CGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGGTAGTAAGT                                                                   >  2:2046940/1‑76              GCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGGTAGTAAGTG                                                                  <  2:1571116/76‑1                 CGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGGTAGTAAGTGCTT                                                               >  2:440962/1‑76                 CGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGGTAGTAAGTGCTT                                                               >  1:1008505/1‑76                   GCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCAGTAGGTAGTAAGTGCTTCA                                                             <  2:1078815/76‑1                     AACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATT                                                           >  2:38307/1‑76                      ACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTT                                                          >  1:1506405/1‑76                      ACGCCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTT                                                          <  1:1155006/76‑1                       CGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTT                                                         >  2:356481/1‑76                           CTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTC                                                     >  1:699250/1‑76                               TGAGTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGC                                                 >  1:317188/1‑76                               TGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTTGATCGTGC                                                 >  1:1292885/1‑76                                GACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGCG                                                >  2:963363/1‑76                                GACGAAGTCCTAGTGGTTCGCGAGCATTACGCGGGCGGGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGCG                                                <  1:475856/76‑1                                 ACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGCGT                                               >  2:1361659/1‑76                                   TAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGG                                             <  1:1462833/76‑1                                   TAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGG                                             >  1:1537366/1‑76                                   TAAGGCCTAGTTGTTCGCGAGAATTACGCAGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGG                                             <  1:1462844/76‑1                                    AAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGC                                            >  1:1160389/1‑76                                    AAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGC                                            >  2:1402764/1‑76                                         CTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAG                                       >  2:915321/1‑76                                          TAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGT                                      <  2:1828786/76‑1                                           AGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTA                                     >  1:2002582/1‑76                                            GTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTAT                                    >  2:683727/1‑76                                             TTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATT                                   <  2:473948/76‑1                                             TTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATT                                   >  2:1665219/1‑76                                             TTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATT                                   >  1:733928/1‑76                                               GTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCA                                 >  1:1416800/1‑76                                               GTTCGCGAGATTTACGCAGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCA                                 <  1:1264586/76‑1                                                TTCGCGAGCATTACGCAGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCAT                                >  2:1411191/1‑76                                                     GAGCATTACGCAGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCT                           >  2:182295/1‑76                                                      AGCATTACGCAGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTG                          <  2:1431652/76‑1                                                       GCATTACGCGGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGC                         <  1:1635490/76‑1                                                        CATTACGCAGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGGGCGGGGCTTCAGTATTCATTCGCTGCc                        >  1:1157382/1‑75                                                          TTACGCGGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGG                      <  1:574896/76‑1                                                          TTACGCAGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGG                      >  2:192372/1‑76                                                           TACGCAGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGT                     >  1:778289/1‑76                                                             CGCGGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGCTTGGCTTCAGTATTCATTCGCTGCAGGTAC                   <  2:54698/76‑1                                                             CGCGGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTAC                   <  2:69807/76‑1                                                             CGCAGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTAC                   <  2:614167/76‑1                                                              GCAGGCGAGCGCCGGTAGGTAGTAAGTGTTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACG                  >  1:1404722/1‑76                                                              GCAGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACG                  >  1:1712752/1‑76                                                                AGGCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTT                >  2:1470879/1‑76                                                                  GCGAGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTA              >  2:1865936/1‑76                                                                     AGCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAAT           <  2:1691844/76‑1                                                                      GCGCCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATG          <  1:360189/76‑1                                                                         CCGGTAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGA       >  2:1099464/1‑76                                                                            GTAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATT    >  1:1182233/1‑76                                                                            GTAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATT    <  1:782967/76‑1                                                                            GTAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATT    <  2:1958882/76‑1                                                                             TAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTG   >  2:1310532/1‑76                                                                             TAGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTG   >  1:471883/1‑76                                                                              AGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGA  >  2:62337/1‑76                                                                              AGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGA  >  1:161038/1‑76                                                                           ||||                                                                            TTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/2563545‑2563618 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGA  >  REL606/2563619‑2563693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.