New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	3022554 =	41 (0.820)	3 (0.070)	3/136	5.8	7.4%	coding (131/1140 nt)	ECB_02821	hypothetical protein
	?	REL606	3022717 =	37 (0.810)					intergenic (‑33/+541)	ECB_02821/ECB_02822	hypothetical protein/bifunctional Ribulose 5‑phosphate reductase/CDP‑ribitol pyrophosphorylase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCATTTGAGCAACTTATTCGTTACTTTCAATCACAAGTATTTAATAACAAGGATTGTATTAGTGTTTTTTGTAAATATTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/3022633‑3022554 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑atattaACATCTATTTATTTATGATATCTCACAGGGTTTAATATTTTATAAATGTTATTAACAAGACCAGCAGGTTTATC  >  REL606/3022717‑3022790                                                                                                                                                            GCATTTGAGCAACTTATTCGTTACTTTCAATCACAAGTATTTAATAACAAGGATTGTATTAGTGTTTTTTGTAAA                                                                                 >  2:1526666/1‑75  CATTTGAGCAACTTATTCGTTACTTTCAATCACAAGTATTTAATAACAAGGATTGTATTAGTGTTTTTTGTAAAT                                                                                <  2:1249606/75‑1                          TTCAATCACAAGTATTTAATAACAAGGATTGTATTAGTGTTTTTTGTAAATATTAACATCTATTTATTTATGATA                                                        <  2:1315228/75‑1                                     GTATTTAATAACAAGGATTGTATTAGTGTTTTTTGTAAATATTAACATCTATTTATTTATGATATCTCACAGGGT                                             <  2:198591/75‑1                                             TAACAAGGATTGTATTAGTGTTTTTTGTAAATATTAACATCTATTTATTTATGATATCTCACAGGGTTTAATATT                                     <  2:518721/75‑1                                                                            TATTAACATCTATTTATTTATGATATCTCACAGGGTTTAATATTTTATAAATGTTATTAACAAGACCAGCAGGTT      <  2:1476211/75‑1                                                                               TAACATCTATTTATTTATGATATCTCACAGGGTTTAATATTTTATAAATGTTATTAACAAGACCAGCAGGTTTAT   >  1:1192426/1‑75                                                                               TAACATCTATTTATTTATGATATCTCACAGGGTTTAATATTTTATAAATGTTATTAACAAGACCAGCAGGTTTAT   >  1:1192452/1‑75                                                                               TAACATCTATTTATTTATGATATCTCACAGGGTTTAATATTTTATAAATGTTATTAACAAGACCAGCAGGTTTAT   <  2:964148/75‑1                                                                               TAACATCTATTTATTTATGATATCTAACAGGGTTTAATATTTTATAAATGTTATTAACAAGACCAGCAGGTTTAT   >  1:1192433/1‑75                                                                                AACATCTATTTATTTATGATATCTCACAGGGTTTAATATTTTATAAATGTTATTAACAAGACCAGCAGGTTTATC  >  2:485175/1‑75                                                                                                                                                            GCATTTGAGCAACTTATTCGTTACTTTCAATCACAAGTATTTAATAACAAGGATTGTATTAGTGTTTTTTGTAAATATTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/3022633‑3022554 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑atattaACATCTATTTATTTATGATATCTCACAGGGTTTAATATTTTATAAATGTTATTAACAAGACCAGCAGGTTTATC  >  REL606/3022717‑3022790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.