Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	REL606	3,014,068	(AAC)3→2	coding (649‑651/1137 nt)	ECB_02815 →	Capsule polysaccharide export inner‑membrane protein kpsE

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	3014063 =	2 (0.030)	60 (1.150)	49/134	0.1	96.9%	coding (644/1137 nt)	ECB_02815	Capsule polysaccharide export inner‑membrane protein kpsE
	?	REL606	= 3014059	2 (0.040)					coding (640/1137 nt)	ECB_02815	Capsule polysaccharide export inner‑membrane protein kpsE

ATCTGGATCTTCTGGCCCATCAGCGTATTCACTAACGTGCTCGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/3014144‑3014063 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gttgttgtCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACGCTGGCGTGCCTTTTCCATCTCCGTTTCTGCAAAGG  <  REL606/3014059‑3013985                                                                                                                                                               ATCTGGATCTTCTGGCCCATCAGCGTATTCACTAACGTGCTCGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGT                                                                                   >  2:1682551/1‑76    TGGATCTTCTGGCCCATCAGCGTATTCACTAACGTGCTCGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGT                                                                                >  2:12828/1‑76    TGGATCTTCTGGCCCATCAGCGTATTCACTAACGTGCTCGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGT                                                                                <  1:1562969/76‑1      GATCTTCTGGCCCATCAGCGTATTCACTAACGTGCTCGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTG                                                                              <  1:490747/76‑1       ATCTTCTGGCCCATCAGCGTATTCACTAACGTGCTCGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGT                                                                             >  1:629971/1‑76          TTCTGGCCCATCAGCGTATTCACTAACGTGCTCGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTT                                                                          <  2:1717607/76‑1           TCTGGCCCATCAGCGTATTCACTAACGTGCTCGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTG                                                                         <  2:1033561/76‑1            CTGGCCCATCAGCGTATTCACTAACGTGCTCGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGA                                                                        >  2:1776758/1‑76             TGGCCCATCAGCGTATTCACTAACGTGCTCGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGAT                                                                       <  1:533741/76‑1              GGCCCATCAGCGTATTCACTAACGTGCTCGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATA                                                                      >  1:1353385/1‑76               GCCCATCAGCGTATTCACTAACGTGCTCGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAA                                                                     <  2:852034/76‑1                CCCATCAGCGTATTCACTAACGTGCTCGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAG                                                                    >  1:1100381/1‑76                CCCATCAGCGTATTCACTAACGTGCTCGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAG                                                                    <  2:1152661/76‑1                  CATCAGCGTATTCACTAACGTGCTCGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAG                                                                  <  2:1186723/76‑1                     CAGCGTATTCACTAACGTGCTCGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGC                                                               <  1:38679/76‑1                      AGCGTATTCACTAACGTGCTCGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCA                                                              >  1:687452/1‑76                      AGCGTATTCACTAACGTGCTCGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCA                                                              <  1:1860340/76‑1                        CGTATTCACTAACGTGCTCGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAAT                                                            >  2:776892/1‑76                          TATTCACTAACGTGCTCGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTC                                                          >  2:1734681/1‑76                          TATTCACTAACGTGCTCGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTC                                                          >  2:1340229/1‑76                          TATTCACTAACGTGCTCGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTC                                                          >  1:812254/1‑76                           ATTCACTAACGTGCTCGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCC                                                         >  1:1744489/1‑76                           ATTCACTAACGTGCTCGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCC                                                         >  1:530288/1‑76                              CACTAACGTGCTCGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCT                                                      >  1:834839/1‑76                                CTAACGTGCTCGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTT                                                    <  1:303002/76‑1                                CTAACGTGCTCGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTT                                                    >  1:687650/1‑76                                CTAACGTGCTCGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTT                                                    <  2:877473/76‑1                                 TAACGTGCTCGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTG                                                   >  1:49484/1‑76                                  AACGTGCTCGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGC                                                  >  2:1356390/1‑76                                    CGTGCTCGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTG                                                <  2:1215765/76‑1                                        CTCGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGT                                            >  1:1813766/1‑76                                          CGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCC                                          >  2:503107/1‑76                                          CGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCC                                          >  2:1367043/1‑76                                          CGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCC                                          >  2:1359703/1‑76                                           GCCGCATGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCA                                         <  2:708279/76‑1                                            CCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAG                                        >  2:248397/1‑76                                             CGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGA                                       >  1:628081/1‑76                                              GCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGAC                                      >  1:137012/1‑76                                              GCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGAC                                      >  1:1563008/1‑76                                               CCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACG                                     <  1:268341/76‑1                                               CCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACG                                     >  2:1167981/1‑76                                                CTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACGC                                    >  1:214888/1‑76                                                 TGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACGCT                                   <  2:530288/76‑1                                                 TGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACGCT                                   <  2:1744489/76‑1                                                  GTGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACGCTG                                  <  1:623198/76‑1                                                   TGCCTGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACGCTGG                                 <  2:335939/76‑1                                                       TGTGCCTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACGCTGGCGTG                             >  1:1394375/1‑76                                                            CTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACGCTGGCGTGCCTTT                        >  1:1494388/1‑76                                                            CTGTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACGCTGGCGTGCCTTT                        >  2:364252/1‑76                                                              GTGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACGCTGGCGTGCCTTTTC                      >  2:402824/1‑76                                                               TGGATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACGCTGGCGTGCCTTTTCC                     >  2:1471618/1‑76                                                                 GATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACGCTGGCGTGCCTTTTCCAT                   >  1:615922/1‑76                                                                 GATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACGCTGGCGTGCCTTTTCCAT                   <  2:453362/76‑1                                                                 GATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACGCTGGCGTGCCTTTTCCAT                   <  1:1476152/76‑1                                                                  ATCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACGCTGGCGTGCCTTTTCCATC                  >  2:1109988/1‑76                                                                   TCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACGCTGGCGTGCCTTTTCCATCT                 <  1:171806/76‑1                                                                   TCCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACGCTGGCGTGCCCTTTTCCTCT                 >  1:719761/1‑76                                                                    CCAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACGCTGGCGTGCCCTTTCCCTCTC                >  2:1080860/1‑76                                                                     CAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACGCTGGCGTGCCTTTTCCATCTCC               >  2:747761/1‑76                                                                     CAGAACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACGCTGGCGTGCCTTTTCCATCTCC               >  2:361345/1‑76                                                                     CAGAACGTTGTTGTCTTGATAAGAGAGCAATGCCGCTTTGCTGGCGTCCAGACGCTGGCGTGCCTTTTCCATCTCC               <  2:301662/76‑1                                                                        AACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACGCTGGCGTGCCTTTTCCATCTCCGTT            >  1:240755/1‑76                                                                         ACGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACGCTGGCGTGCCTTTTTCATCTCCCTTT           >  1:1720354/1‑76                                                                          CGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACGCTGGCGTGCCTTTTCCATCTCCGTTTC          >  1:1013302/1‑76                                                                          CGTTGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACGCTGGCGTGCCTTTTCCATCTCCGTTTC          <  2:385791/76‑1                                                                             TGTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACGCTGGCGTGCCTTTTCCATCTCCGTTTCTGC       >  1:1577520/1‑76                                                                              GTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACGCTGGCGTGCCTTTTCCATCTCCGTTTCTGCA      >  2:299753/1‑76                                                                              GTTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACGCTGGCGTGCCTTTTCCATCTCCGTTTCTGCA      >  2:1788892/1‑76                                                                               TTGTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACGCTGGCGTGCCTTTTCCATCTCCGTTTCTGCAA     >  1:583136/1‑76                                                                                 GTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACGCTGGCGTGCCTTTTCCATCTCCGTTTCTGCAAAG   <  2:238825/76‑1                                                                                 GTCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACGCTGGCGTGCCTTTTCCATCTCCGTTTCTGCAAAG   <  1:1143672/76‑1                                                                                  TCTTGATAAGAGAGCCATTCCGCTTTGCGGGCGTCCAGACGCTGGCGTGCCTTTTCCATCTCCGTTTCTGCAAAGG  <  1:1834721/76‑1                                                                                  TCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACGCTGGCGTGCCTTTTCCATCTCCGTTTCTGCAAAGG  >  2:347926/1‑76                                                                                  TCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACGCTGGCGTGCCTTTTCCATCTCCGTTTCTGCAAAGG  >  1:227469/1‑76                                                                                                                                                               ATCTGGATCTTCTGGCCCATCAGCGTATTCACTAACGTGCTCGCCGCCTGTGCCTGTGCCTGTGGATCCAGAACGTTGTTGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/3014144‑3014063 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gttgttgtCTTGATAAGAGAGCAATTCCGCTTTGCTGGCGTCCAGACGCTGGCGTGCCTTTTCCATCTCCGTTTCTGCAAAGG  <  REL606/3014059‑3013985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.