New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 2040272	54 (0.810)	5 (0.080)	4/138	8.4	10.6%	coding (1112/1113 nt)	vioA	VioA, involved in dTDP‑N‑acetylviosamine synthesis
	?	REL606	= 2040459	34 (0.550)					coding (925/1113 nt)	vioA	VioA, involved in dTDP‑N‑acetylviosamine synthesis
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CTGATATTTTTACATTTTCTCCAACGCTTTTGAAGCCCATTTCTTTTAACTGTATTTCATCTAAATAGGCCATATC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/2040197‑2040272 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑atatcCTATCTCGAAAGTATTTTTATCCATTAATCAGTAATATGCCTATGTATCGAGGATTGATTTCAG  <  REL606/2040459‑2040396                                                                                                                                              CTGATATTTTTACATTTTCTCCAACGCTTTTGAAGCCCATTTCTTTTAACTGTATTTCATCTAAATAGGCCATAT                                                                   <  1:1009971/75‑1 CTGATATTTTTACATTTTCTCCAACGCTTTTGAAGCCCATTTCTTTTAACTGTATTTCATCTAAATAGGCCATAT                                                                   <  1:1009991/75‑1 CTGATATTTTTACATTTTCTCCAACGCTTTTGAAGCCCATTTCTTTTAACTGTATTTCATCTAAATAGGCCATAT                                                                   >  2:155595/1‑75                                                 ACTGTATTTCATCTAAATAGGCCATATCCTATCTCGAAAGTATTTTTATCCATTAATCAGTAATATGCCTATGTA                   >  2:14355/1‑75                                                    GTATTTCATCTAAATAGGCCATATCCTATCTCGAAAGTATTTTTATCCATTAATCAGTAATATGCCTATGTATCG                >  2:1207878/1‑75                                                        TTCATCTAAATAGGCCATATCCTATCTCGAAAGTATTTTTATCCATTAATCAGTAATATGCCTATGTATCGAGGA            >  2:1862757/1‑75                                                                  TAGGCCATATCCTATCTCGAAAGTATTTTTATCCATTAATCAGTAATATGCCTATGTATCGAGGATTGATTTCAG  >  2:412487/1‑75                                                                  TAGGCCATATCCTATCTCGAAAGTATTTTTATCCATTAATCAGTAATATGCCTATGTATCGAGGATTGATTTCAG  >  2:412498/1‑75                                                                                                                                              CTGATATTTTTACATTTTCTCCAACGCTTTTGAAGCCCATTTCTTTTAACTGTATTTCATCTAAATAGGCCATATC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/2040197‑2040272 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑atatcCTATCTCGAAAGTATTTTTATCCATTAATCAGTAATATGCCTATGTATCGAGGATTGATTTCAG  <  REL606/2040459‑2040396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.