New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 2040272	54 (0.810)	5 (0.080)	4/138	8.4	10.6%	coding (1112/1113 nt)	vioA	VioA, involved in dTDP‑N‑acetylviosamine synthesis
	?	REL606	= 2040459	34 (0.550)					coding (925/1113 nt)	vioA	VioA, involved in dTDP‑N‑acetylviosamine synthesis
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GATATTTTTACATTTTCTCCAACGCTTTTGAAGCCCATTTCTTTTAACTGTATTTCATCTAAATAGGCCATATCACATCTTACCCAATAATAATTTGGTTATTTTCACAACAATCTCTTCATTTAAATCTGTATATATTGGCAAGCAT  >  REL606/2040199‑2040346                                                                          |                                                                           gATATTTTTACATTTTCTCCAACGCTTTTGAAGCCCATTTCTTTTAACTGTATTTCATCTAAATAGGCCATATca                                                                           <  1:1364722/75‑1 (MQ=255) gATATTTTTACATTTTCTCCAACGCTTTTGAAGCCCATTTCTTTTAACTGTATTTCATCTAAATAGGCCATATca                                                                           <  1:1364704/75‑1 (MQ=255)  atatTTTTACATTTTCTCCAACGCTTTTGAAGCCCATTTCTTTTAACTGTATTTCATCTAAATAGGCCATATcac                                                                          >  2:1248742/1‑75 (MQ=255)   tatTTTTACATTTTCTCCAACGCTTTTGAAGCCCATTTCTTTTAACTGTATTTCATCTAAATAGGCCATATcaca                                                                         <  1:1398308/75‑1 (MQ=255)   tatTTTTACATTTTCTCCAACGCTTTTGAAGCCCATTTCTTTTAACTGTATTTCATCTAAATAGGCCATATcaca                                                                         <  1:1398284/75‑1 (MQ=255)     tttttACATTTTCTCCAACGCTTTTGAAGCCCATTTCTTTTAACTGTATTTCATCTAAATAGGCCATATCACATc                                                                       <  2:559954/75‑1 (MQ=255)       tttACATTTTCTCCAACGCTTTTGAAGCCCATTTCTTTTAACTGTATTTCATCTAAATAGGCCATATCACATCtt                                                                     <  2:1985386/75‑1 (MQ=255)           cATTTTCTCCAACGCTTTTGAAGCCCATTTCTTTTAACTGTATTTCATCTAAATAGGCCATATCACATCTTAccc                                                                 <  2:1047926/75‑1 (MQ=255)             ttttCTCCAACGCTTTTGAAGCCCATTTCTTTTAACTGTATTTCATCTAAATAGGCCATATCACATCTTACCCaa                                                               <  1:1959002/75‑1 (MQ=255)                tctcCAACGCTTTTGAAGCCCATTTCTTTTAACTGTATTTCATCTAAATAGGCCATATCACATCTTACCCaataa                                                            <  2:883909/75‑1 (MQ=255)                       cGCTTTTGAAGCCCATTTCTTTTAACTGTATTTCATCTAAATAGGCCATATCACATCTTACCCAATAATAATTTg                                                     <  2:144797/75‑1 (MQ=255)                        gCTTTTGAAGCCCATTTCTTTTAACTGTATTTCATCTAAATAGGCCATATCACATCTTACCCAATAATAATTTgg                                                    >  2:156230/1‑75 (MQ=255)                           tttGAAGCCCATTTCTTTTAACTGTATTTCATCTAAATAGGCCATATCACATCTTACCCAATAATAATTTGGTTa                                                 >  1:1801713/1‑75 (MQ=255)                            ttGAAGCCCATTTCTTTTAACTGTATTTCATCTAAATAGGCCATATCACATCTTACCCAATAATAATTTGGTTAt                                                >  1:1460499/1‑75 (MQ=255)                             tGAAGCCCATTTCTTTTAACTGTATTTCATCTAAATAGGCCATATCACATCTTACCCAATAATAATTTGGTTAtt                                               <  1:1747627/75‑1 (MQ=255)                                 gCCCATTTCTTTTAACTGTATTTCATCTAAATAGGCCATATCACATCTTACCCAATAATAATTTGGTTATTTTca                                           >  1:1186251/1‑75 (MQ=255)                                   ccATTTCTTTTAACTGTATTTCATCTAAATAGGCCATATCACATCTTACCCAATAATAATTTGGTTATTTTcaca                                         >  1:397172/1‑75 (MQ=255)                                     aTTTCTTTTAACTGTATTTCATCTAAATAGGCCATATCACATCTTACCCAATAATAATTTGGTTATTTTCacaac                                       >  1:784353/1‑75 (MQ=255)                                     aTTTCTTTTAACTGTATTTCATCTAAATAGGCCATATCACATCTTACCCAATAATAATTTGGTTATTTTCacaac                                       <  1:1859165/75‑1 (MQ=255)                                     aTTTCTTTTAACTGTATTTCATCTAAATAGGCCATATCACATCTTACCCAATAATAATTTGGATATTTTCacaac                                       >  1:15054/1‑75 (MQ=255)                                        tCTTTTAACTGTATTTCATCTAAATAGGCCATATCTCATCTTACCCAATAATAATTTGGTTATTTTCACAACAAt                                    <  1:2070149/75‑1 (MQ=255)                                          ttttAACTGTATTTCATCTAAATAGGCCATATCACATCTTACCCAATAATAATTTGGCTATTTTCACAACAAtct                                  >  1:1307730/1‑75 (MQ=255)                                           tttAACTGTATTTCATCTAAATAGGCCATATCACATCTTACCCAATAATACTTTGGTTATTTTCACAACAAtctc                                 >  1:1232889/1‑75 (MQ=255)                                           tttAACTGTATTTCATCTAAATAGGCCATATCACATCTTACCCAATAATAATTTGGTTATTTTCACAACAAtctc                                 >  1:1232902/1‑75 (MQ=255)                                           tttAACTGTATTTCATCTAAATAGGCCATATCACATCTTACCCAATAATAATTTGGTTATTTTCACAACAAtctc                                 <  1:519031/75‑1 (MQ=255)                                           tttAACTGTATTTCATCTAAATAGGCCATATCACATCTTACCCAATAATAATTTGGTTATTTTCACAACAAtctc                                 >  1:1640106/1‑75 (MQ=255)                                            ttAACTGTATTTCATCTAAATAGGCCATATCACATCTTACCCAATAATAATTTGGTTATTTTCACAACAATctct                                <  1:942515/75‑1 (MQ=255)                                            ttAACTGTATTTCATCTAAATAGGCCATATCACATCTTACCCAATAATAATTTGGTTATTTTCACAACAATctct                                <  1:942491/75‑1 (MQ=255)                                             tAACTGTATTTCATCTAAATAGGCCATATCACATCTTACCCAATAATAATTTGGTTATTTTCACAACAATCTCtt                               <  1:1180453/75‑1 (MQ=255)                                             tAACTGTATTTCATCTAAATAGGCCATATCACATCTTACCCAATAATAATTTGGTTATTTTCACAACAATCTCtt                               <  1:1180468/75‑1 (MQ=255)                                              aaCTGTATTTCATCTAAATAGGCCATATCACATCTTACCCAATAATAATTTGGTTATTTTCACAACAATCTCTTc                              >  2:2151005/1‑75 (MQ=255)                                               aCTGTATTTCATCTAAATAGGCCATATCACATCTTACCCAATAATAATTTGGTTATTTTCACAACAATCTCTTCa                             >  1:14355/1‑75 (MQ=255)                                                cTGTATTTCATCTAAATAGGCCATATCACATCTTACCCAATAATAATTTGGTTATTTTCACAACAATCTCTTCAt                            <  1:1190228/75‑1 (MQ=255)                                                  gTATTTCATCTAAATAGGCCATATCACATCTTACCCAATAATAATTTGGTTATTTTCACAACAATCTCTTCAttt                          >  1:1207878/1‑75 (MQ=255)                                                      ttCATCTAAATAGGCCATATCACATCTTACCCAATAATAATTTGGTTATTTTCACAACAATCTCTTCATTTAAAt                      >  2:547865/1‑75 (MQ=255)                                                      ttCATCTAAATAGGCCATATCACATCTTACCCAATAATAATTTGGTTATTTTCACAACAATCTCTTCATTTAAAt                      >  1:1862757/1‑75 (MQ=255)                                                       tCATCTAAATAGGCCATATCACATCTTACCCAATAATAATTTGGTTATTTTCACAACAATCTCTTCATTTAAATc                     >  2:1514015/1‑75 (MQ=255)                                                        cATCTAAATAGGCCATATCACATCTTACCCAATAATAATTTGGGTATTTTCACAACAAGCTCTTCATTTAAATCt                    <  1:643940/75‑1 (MQ=255)                                                              aaTAGGCCATATCACATCTTACCCAATAATAATTTGGTTATTTTCACAACAATCTCTTCATTTAAATCTGtatat              >  2:875119/1‑75 (MQ=255)                                                                tAGGCCATATCACATCTTACCCAATAATAATTTGGTTATTTTCACAACAATCTCTTCATTTAAATCTGTatatat            >  1:412498/1‑75 (MQ=255)                                                                tAGGCCATATCACATCTTACCCAATAATAATTTGGTTATTTTCACAACAATCTCTTCATTTAAATCTGTatatat            >  1:412487/1‑75 (MQ=255)                                                                  ggCCATATCACATCTTACCCAATAATAATTTGGTTATTTTCACAACAATCTCTTCATTTAAATCTGTATATATTg          >  1:529317/1‑75 (MQ=255)                                                                  ggCCATATCACATCTTACCCAATAATAATTTGGTTATTTTCACAACAATCTCTTCATTTAAATCTGTATATATTg          <  1:1004403/75‑1 (MQ=255)                                                                    ccATATCACATCTTACCCAATAATAATTTGGTTATTTTCACAACAATCTCTTCATTTAAATCTGTATATATTGGc        <  1:666850/75‑1 (MQ=255)                                                                    ccATATCACATCTTACCCAATAATAATTTGGTTATTTTCACAACAATCTCTTCATTTAAATCTGTATATATTGGc        <  1:666857/75‑1 (MQ=255)                                                                       tatCACATCTTACCCAATAATAATTTGGTTATTTTCACAACAATCTCTTCATTTAAATCTGTATATATTGGCAAg     >  1:543045/1‑75 (MQ=255)                                                                         tCACATCTTACCCAATAATAATTTGGTTATTTTCACAACAATCTCTTCATTTAAATCTGTATATATTGGCaaagg   <  2:1964902/75‑4 (MQ=255)                                                                         tCACATCTTACCCAATAATAATTTGGTTATTTTCACAACAATCTCTTCATTTAAATCTGTATATATTGGCAAGCa   <  1:1838008/75‑1 (MQ=255)                                                                         tCACATCTTACCCAATAATAATTTGGTTATTTTCACAACAATCTCTTCATTTAAATCTGTATATATTGGCAAGCa   <  1:1813825/75‑1 (MQ=255)                                                                         tCACATCTTACCCAATAATAATTTGGTTATTTTCACAACAATCTCTTCATTTAAATCTGTATATATTGGCAAGCa   <  1:1813808/75‑1 (MQ=255)                                                                          cacaTCTTACCCAATAATAATTTGGTTATTTTCACAACAATCTCTTCATTTAAATCTGTATATATTGGCAAGCAt  <  1:359167/75‑1 (MQ=255)                                                                          cacaTCTTACCCAATAATAATTTGGTTATTTTCACAACAATCTCTTCATTTAAATCTGTATATATTGGCAAGCAt  <  2:165030/75‑1 (MQ=255)                                                                          cacaTCTTACCCAATAATAATTTGGTTATTTTCACAACAATCTCTTCATTTAAATCTGTATATATTGGCAAGCAt  <  2:165009/75‑1 (MQ=255)                                                                          cacaTCTTACCCAATAATAATTTGGTTATTTTCACAACAATCTCTTCATTTAAATCTGTATATATTGGCAAGCAt  <  1:1012073/75‑1 (MQ=255)                                                                          |                                                                           GATATTTTTACATTTTCTCCAACGCTTTTGAAGCCCATTTCTTTTAACTGTATTTCATCTAAATAGGCCATATCACATCTTACCCAATAATAATTTGGTTATTTTCACAACAATCTCTTCATTTAAATCTGTATATATTGGCAAGCAT  >  REL606/2040199‑2040346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.