Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	REL606	3,339,054	Δ5 bp	coding (884‑888/966 nt)	yhdG →	tRNA‑dihydrouridine synthase B

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 3339053	1 (0.020)	73 (1.240)	49/150	0.1	98.7%	coding (883/966 nt)	yhdG	tRNA‑dihydrouridine synthase B
	?	REL606	3339059 =	1 (0.020)					coding (889/966 nt)	yhdG	tRNA‑dihydrouridine synthase B

GTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/3338982‑3339053 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCG  >  REL606/3339059‑3339133                                                                                                                                                     GTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGC                                                                         >  1:1455137/1‑76     GGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCAC                                                                     <  2:1848847/76‑1       CAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCACAT                                                                   >  2:898963/1‑76       CAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCACAT                                                                   >  2:528187/1‑76       CAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCACAT                                                                   <  2:485960/76‑1       CAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCACAT                                                                   >  1:1795641/1‑76        AAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATT                                                                  <  2:1455137/76‑1         aagGGGTACCGAATTGCCGGTAACCACGTTTCCGGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTC                                                                 <  1:922617/73‑1         AAAGGGTCCCGAATGGCATGTAAACCCATTTGCTGGTATCCCCAGGAACACGCTCCAAATGACCCGGCGCACATTC                                                                 <  1:1168185/76‑1         AAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTC                                                                 <  1:1336682/76‑1          AAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTCA                                                                >  2:490780/1‑76           AGGGTACCGAATCGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAA                                                               <  1:1375356/76‑1            GGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAAC                                                              <  1:462336/76‑1             GGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACG                                                             <  2:657787/76‑1             GGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACG                                                             <  2:1251728/76‑1               TACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCC                                                           <  2:578514/76‑1                 CCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCAT                                                         >  1:1791613/1‑76                  CGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATT                                                        >  2:1320417/1‑76                   GAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTG                                                       >  1:1081368/1‑76                    AATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGA                                                      >  2:1253888/1‑76                      TTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGG                                                    <  2:313108/76‑1                         CACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATG                                                 >  2:764529/1‑76                           CGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCC                                               <  2:51164/76‑1                            GTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCA                                              >  1:842632/1‑76                            GTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCA                                              >  2:1164884/1‑76                             TAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCCCATTCACCGCCATTGAGGCTGCCAG                                             >  2:1632978/1‑76                             TAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAG                                             <  2:1791612/76‑1                              AAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGGTGCCAGC                                            >  2:1136484/1‑76                              AAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGGTGCCAGC                                            >  2:1136495/1‑76                              AAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGC                                            >  2:242715/1‑76                              AAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGC                                            <  1:395521/76‑1                              AAACACGTTTCCTGGTATCTCCAGGAAAACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGC                                            >  2:22196/1‑76                                ACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGA                                          <  2:265929/76‑1                                 CACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAA                                         >  1:554392/1‑76                                 CACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAA                                         >  2:204206/1‑76                                      TTCCTGGTAACTCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCT                                    <  2:861242/76‑1                                       TCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTG                                   >  2:720139/1‑76                                       TCCTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTG                                   >  2:696658/1‑76                                         CTGGTATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGA                                 <  1:221493/76‑1                                             TATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGata                             >  1:1003127/1‑73                                             TATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGata                             <  2:1003127/76‑4                                             TATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCG                             >  1:1297368/1‑76                                             TATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCG                             <  2:1282270/76‑1                                             TATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCG                             <  1:938187/76‑1                                             TATCTCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCG                             >  1:792638/1‑76                                                CTCGAGGAACACGCTCCAAATGACCTGGCGCGCATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTG                          <  2:379090/76‑1                                                 cCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGG                         <  2:451806/75‑1                                                 cCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGG                         <  1:859606/75‑1                                                 TCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGG                         <  2:423954/76‑1                                                 TCCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGG                         <  2:1501561/76‑1                                                  CCAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGCGGCGTTGGA                        >  2:151219/1‑76                                                   CAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAG                       >  1:1713256/1‑76                                                   CAGGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAG                       <  2:1362839/76‑1                                                   CAGGAACACGCTCAAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAG                       >  1:652569/1‑76                                                     GGAACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGg                     >  2:1445809/1‑75                                                       AACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGGGAACAGCTGGAGGCGTTGGAGGCAT                   <  1:254347/76‑1                                                       AACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCAT                   <  1:1402313/76‑1                                                       AACACGCTCCAAATCACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCAT                   <  1:778568/76‑1                                                        ACACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATA                  >  2:500427/1‑76                                                         CACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATAC                 <  2:1729063/76‑1                                                         CACGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATAC                 >  2:82691/1‑76                                                           CGCTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTT               <  2:1717908/76‑1                                                             CTCCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCG             >  2:1378882/1‑76                                                               CCAAATTACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCGAA           <  2:1774730/76‑1                                                               CCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCGAA           >  2:39079/1‑76                                                               CCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCGAA           >  2:677805/1‑76                                                               CCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCGAA           <  1:477140/76‑1                                                               CCAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCGAA           >  1:1273569/1‑76                                                                CAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCGAAA          >  2:1621261/1‑76                                                                CAAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCGAAA          <  1:1149245/76‑1                                                                 AAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCGAAAA         <  2:696247/76‑1                                                                 AAATGACCCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCGAAAA         >  2:1654468/1‑76                                                                        CCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCG  >  1:1045350/1‑76                                                                                                                                                     GTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/3338982‑3339053 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCG  >  REL606/3339059‑3339133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.