| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| JC | REL606 | 3,339,054 | Δ5 bp | coding (884‑888/966 nt) | yhdG → | tRNA‑dihydrouridine synthase B |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | REL606 | = 3339053 | 1 (0.020) | 73 (1.240) | 49/150 | 0.1 | 98.7% | coding (883/966 nt) | yhdG | tRNA‑dihydrouridine synthase B |
| ? | REL606 | 3339059 = | 1 (0.020) | coding (889/966 nt) | yhdG | tRNA‑dihydrouridine synthase B | |||||
GTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATT > REL606/3338982‑3339128 | gTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggc > 1:1455137‑M1/1‑72 (MQ=255) ggCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcac < 2:1848847‑M1/76‑9 (MQ=255) cAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcacat > 2:898963‑M1/1‑66 (MQ=255) cAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcacat > 1:1795641‑M1/1‑66 (MQ=255) cAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcacat > 2:528187‑M1/1‑66 (MQ=255) cAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcacat < 2:485960‑M1/76‑11 (MQ=255) aaaaGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcacatt < 2:1455137‑M1/76‑12 (MQ=255) aaggggtacCGAATTGCCGGTAACCACGTTTCCGGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcacattc < 1:922617‑M1/73‑13 (MQ=255) aaaGGGTCCCGAATGGCATGTAAACCCATTTGCTGGTATCCCCAGGAACACGCTCCAAATGACCcggcgcacattc < 1:1168185‑M1/76‑13 (MQ=255) aaaGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcacattc < 1:1336682‑M1/76‑13 (MQ=255) aaGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcacattca > 2:490780‑M1/1‑63 (MQ=255) aGGGTACCGAATCGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcacattcaa < 1:1375356‑M1/76‑15 (MQ=255) gggTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcacattcaac < 1:462336‑M1/76‑16 (MQ=255) ggTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcacattcaacg < 2:1251728‑M1/76‑17 (MQ=255) ggTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcacattcaacg < 2:657787‑M1/76‑17 (MQ=255) tACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcacattcaacgcc < 2:578514‑M1/76‑19 (MQ=255) ccGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcacattcaacgccat > 1:1791613‑M1/1‑56 (MQ=255) cGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcacattcaacgccatt > 2:1320417‑M1/1‑55 (MQ=255) gAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcacattcaacgccattg > 1:1081368‑M1/1‑54 (MQ=255) aaTTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcacattcaacgccattga > 2:1253888‑M1/1‑53 (MQ=255) ttGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcacattcaacgccattgagg < 2:313108‑M1/76‑26 (MQ=255) cACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcacattcaacgccattgaggatg > 2:764529‑M1/1‑48 (MQ=255) cGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcacattcaacgccattgaggatgcc < 2:51164‑M1/76‑31 (MQ=255) gTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcacattcaacgccattgaggatgcca > 1:842632‑M1/1‑45 (MQ=255) gTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcacattcaacgccattgaggatgcca > 2:1164884‑M1/1‑45 (MQ=255) tAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcccattcaccgccattgaggctgccag > 2:1632978‑M1/1‑44 (MQ=255) tAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcacattcaacgccattgaggatgccag < 2:1791612‑M1/76‑33 (MQ=255) aaaCACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcacattcaacgccattgagggtgccagc > 2:1136484‑M1/1‑43 (MQ=255) aaaCACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcacattcaacgccattgagggtgccagc > 2:1136495‑M1/1‑43 (MQ=255) aaaCACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcacattcaacgccattgaggatgccagc > 2:242715‑M1/1‑43 (MQ=255) aaaCACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcacattcaacgccattgaggatgccagc < 1:395521‑M1/76‑34 (MQ=255) aaaCACGTTTCCTGGTATCTCCAGGAAAACGCTCCAAATGACCcggcgcacattcaacgccattgaggatgccagc > 2:22196‑M1/1‑43 (MQ=255) acacGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcacattcaacgccattgaggatgccagcga < 2:265929‑M1/76‑36 (MQ=255) cacGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcacattcaacgccattgaggatgccagcgaa > 1:554392‑M1/1‑40 (MQ=255) cacGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcacattcaacgccattgaggatgccagcgaa > 2:204206‑M1/1‑40 (MQ=255) ttCCTGGTAACTCCAGGAACACGCTCCAAATGACCcggcgcacattcaacgccattgaggatgccagcgaacagct < 2:861242‑M1/76‑42 (MQ=255) tCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcacattcaacgccattgaggatgccagcgaacagctg > 2:696658‑M1/1‑34 (MQ=255) tCCTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcacattcaacgccattgaggatgccagcgaacagctg > 2:720139‑M1/1‑34 (MQ=255) cTGGTATCTCCAGGAACACGCTCCAAATGACCcggcgcacattcaacgccattgaggatgccagcgaacagctgga < 1:221493‑M1/76‑45 (MQ=255) tATCTCCAGGAACACGCTCCAAATGACCcggcgcacattcaacgccattgaggatgccagcgaacagctggaggcg < 2:1282270‑M1/76‑49 (MQ=255) tATCTCCAGGAACACGCTCCAAATGACCcggcgcacattcaacgccattgaggatgccagcgaacagctggaggcg > 1:1297368‑M1/1‑28 (MQ=255) tATCTCCAGGAACACGCTCCAAATGACCcggcgcacattcaacgccattgaggatgccagcgaacagctggaggcg > 1:792638‑M1/1‑28 (MQ=255) tATCTCCAGGAACACGCTCCAAATGACCcggcgcacattcaacgccattgaggatgccagcgaacagctggaggcg < 1:938187‑M1/76‑49 (MQ=255) tATCTCCAGGAACACGCTCCAAATGACCcggcgcacattcaacgccattgaggatgccagcgaacagctggagata > 1:1003127‑M1/1‑28 (MQ=255) tATCTCCAGGAACACGCTCCAAATGACCcggcgcacattcaacgccattgaggatgccagcgaacagctggagata < 2:1003127‑M1/76‑49 (MQ=255) ctcGAGGAACACGCTCCAAATGACCtggcgcgcattcaacgccattgaggatgccagcgaacagctggaggcgttg < 2:379090‑M1/76‑52 (MQ=255) tcCAGGAACACGCTCCAAATGACCcggcgcacattcaacgccattgaggatgccagcgaacagctggaggcgttgg < 2:423954‑M1/76‑53 (MQ=255) tcCAGGAACACGCTCCAAATGACCcggcgcacattcaacgccattgaggatgccagcgaacagctggaggcgttgg < 2:1501561‑M1/76‑53 (MQ=255) cccaGGAACACGCTCCAAATGACCcggcgcacattcaacgccattgaggatgccagcgaacagctggaggcgttgg < 2:451806‑M1/75‑53 (MQ=255) cccaGGAACACGCTCCAAATGACCcggcgcacattcaacgccattgaggatgccagcgaacagctggaggcgttgg < 1:859606‑M1/75‑53 (MQ=255) ccAGGAACACGCTCCAAATGACCcggcgcacattcaacgccattgaggatgccagcgaacagctggcggcgttgga > 2:151219‑M1/1‑23 (MQ=255) cAGGAACACGCTCCAAATGACCcggcgcacattcaacgccattgaggatgccagcgaacagctggaggcgttggag > 1:1713256‑M1/1‑22 (MQ=255) cAGGAACACGCTCCAAATGACCcggcgcacattcaacgccattgaggatgccagcgaacagctggaggcgttggag < 2:1362839‑M1/76‑55 (MQ=255) cAGGAACACGCTCAAAATGACCcggcgcacattcaacgccattgaggatgccagcgaacagctggaggcgttggag > 1:652569‑M1/1‑22 (MQ=255) ggAACACGCTCCAAATGACCcggcgcacattcaacgccattgaggatgccagcgaacagctggaggcgttggaggg > 2:1445809‑M1/1‑20 (MQ=255) aaCACGCTCCAAATGACCcggcgcacattcaacgccattgaggatgccagggaacagctggaggcgttggaggcat < 1:254347‑M1/76‑59 (MQ=255) aaCACGCTCCAAATGACCcggcgcacattcaacgccattgaggatgccagcgaacagctggaggcgttggaggcat < 1:1402313‑M1/76‑59 (MQ=255) aaCACGCTCCAAATCACCcggcgcacattcaacgccattgaggatgccagcgaacagctggaggcgttggaggcat < 1:778568‑M1/76‑59 (MQ=255) acacGCTCCAAATGACCcggcgcacattcaacgccattgaggatgccagcgaacagctggaggcgttggaggcata > 2:500427‑M1/1‑17 (MQ=255) cacGCTCCAAATGACCcggcgcacattcaacgccattgaggatgccagcgaacagctggaggcgttggaggcatac < 2:1729063‑M1/76‑61 (MQ=255) cacGCTCCAAATGACCcggcgcacattcaacgccattgaggatgccagcgaacagctggaggcgttggaggcatac > 2:82691‑M1/1‑16 (MQ=255) cGCTCCAAATGACCcggcgcacattcaacgccattgaggatgccagcgaacagctggaggcgttggaggcatactt < 2:1717908‑M1/76‑63 (MQ=255) cTCCAAATGACCcggcgcacattcaacgccattgaggatgccagcgaacagctggaggcgttggaggcatacttcg > 2:1378882‑M1/1‑12 (MQ=255) ccAAATTACCcggcgcacattcaacgccattgaggatgccagcgaacagctggaggcgttggaggcatacttcgaa < 2:1774730‑M1/76‑67 (MQ=255) ccAAATGACCcggcgcacattcaacgccattgaggatgccagcgaacagctggaggcgttggaggcatacttcgaa > 2:39079‑M1/1‑10 (MQ=255) ccAAATGACCcggcgcacattcaacgccattgaggatgccagcgaacagctggaggcgttggaggcatacttcgaa > 2:677805‑M1/1‑10 (MQ=255) ccAAATGACCcggcgcacattcaacgccattgaggatgccagcgaacagctggaggcgttggaggcatacttcgaa < 1:477140‑M1/76‑67 (MQ=255) ccAAATGACCcggcgcacattcaacgccattgaggatgccagcgaacagctggaggcgttggaggcatacttcgaa > 1:1273569‑M1/1‑10 (MQ=255) cAAATGACCcggcgcacattcaacgccattgaggatgccagcgaacagctggaggcgttggaggcatacttcgaaa > 2:1621261‑M1/1‑9 (MQ=255) cAAATGACCcggcgcacattcaacgccattgaggatgccagcgaacagctggaggcgttggaggcatacttcgaaa < 1:1149245‑M1/76‑68 (MQ=255) aaaTGACCcggcgcacattcaacgccattgaggatgccagcgaacagctggaggcgttggaggcatacttcgaaaa > 2:1654468‑M1/1‑8 (MQ=255) aaaTGACCcggcgcacattcaacgccattgaggatgccagcgaacagctggaggcgttggaggcatacttcgaaaa < 2:696247‑M1/76‑69 (MQ=255) aTGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCGa < 1:1246656/76‑1 (MQ=255) ccggcgcacattcaacgccattgaggatgccagcgaacagctggaggcgttggaggcatacttcgaaaattttgcg > 1:1045350‑M1/1‑1 (MQ=255) | GTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATT > REL606/3338982‑3339128 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |