Predicted mutation
evidence seq id position mutation annotation gene description
MC JC REL606 2,904,110 (GTC)4→3 coding (2297‑2299/3099 nt) ygfK → predicted oxidoreductase, Fe‑S subunit

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ REL606 2904101 2904103 3 44 [0] [0] 45 ygfK predicted oxidoreductase, Fe‑S subunit

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 2904104 =0 (0.000)41 (0.960) 29/132 0.4 100% coding (2291/3099 nt) ygfK predicted oxidoreductase, Fe‑S subunit
?REL606 = 2904100 0 (0.000)coding (2287/3099 nt) ygfK predicted oxidoreductase, Fe‑S subunit

CCGGAACGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCA  >  REL606/2904031‑2904179
                                                                         |                                                                           
aatccggaacgtttcgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaaggtc                                                                           <  2:766444‑M1/3‑1 (MQ=255)
aatccggaacgtttcgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaaggtc                                                                           <  1:270797‑M1/3‑1 (MQ=255)
   ccggaacgtttcgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaaggtcgtc                                                                        >  1:407873‑M1/71‑76 (MQ=255)
       aacgtttcgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTcc                                                                    >  1:193500‑M1/67‑76 (MQ=255)
              cgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGaa                                                             <  2:765567‑M1/17‑1 (MQ=255)
              cgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGaa                                                             <  2:139832‑M1/17‑1 (MQ=255)
              cgatgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGaa                                                             <  2:342840‑M1/17‑1 (MQ=255)
                atgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAAc                                                           <  1:377040‑M1/19‑1 (MQ=255)
                 tgctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAAcc                                                          <  1:1258190‑M1/20‑1 (MQ=255)
                  gctgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCa                                                         >  1:749055‑M1/56‑76 (MQ=255)
                    tgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAAt                                                       <  2:475325‑M1/23‑1 (MQ=255)
                    tgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAAt                                                       <  2:7514‑M1/23‑1 (MQ=255)
                    tgatggcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAAt                                                       <  2:1214‑M1/23‑1 (MQ=255)
                         gcaccttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATgaaaa                                                  <  2:543783‑M1/28‑2 (MQ=255)
                             cttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTa                                              <  1:1484160‑M1/32‑1 (MQ=255)
                             cttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTa                                              <  1:1211226‑M1/32‑1 (MQ=255)
                              ttaaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTaa                                             >  2:386040‑M1/44‑76 (MQ=255)
                               caacctcgcgcggtatgacgcttggcgaaccggataagaaacGTCGTCGTCCGGTTGAAACCAATGAAACAGTAac                                            >  1:94555‑M1/43‑76 (MQ=255)
                                aaccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAaca                                           >  2:865842‑M1/42‑76 (MQ=255)
                                  ccttgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACt                                         >  2:267967‑M1/40‑76 (MQ=255)
                                     tgcgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCt                                      <  2:783427‑M1/40‑1 (MQ=255)
                                       cgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTg                                    >  1:1448866‑M1/35‑76 (MQ=255)
                                       cgcgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTg                                    >  1:1291228‑M1/35‑76 (MQ=255)
                                         cgttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTa                                  >  2:890634‑M1/33‑76 (MQ=255)
                                           ttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGa                                <  2:723675‑M1/46‑1 (MQ=255)
                                           ttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGa                                >  1:1041803‑M1/31‑76 (MQ=255)
                                           ttatgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGa                                >  1:949301‑M1/31‑76 (MQ=255)
                                              tgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAg                             >  2:1201429‑M1/28‑76 (MQ=255)
                                              tgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAg                             <  1:1407112‑M1/49‑1 (MQ=255)
                                              tgtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAg                             >  1:1393021‑M1/28‑76 (MQ=255)
                                               gtcgcttggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGc                            <  2:4333‑M1/50‑1 (MQ=255)
                                                      ggcgaaccggatgagaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCa                     >  2:512997‑M1/20‑76 (MQ=255)
                                                        cgaaccggatgagaaagGTCGTCGTCCGGTTGAAACAAATGAAACAGAAACACTGCTTGTAGACAGCCTGATCAcc                   <  2:973010‑M1/59‑1 (MQ=255)
                                                                   agaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGa        <  2:791426‑M1/70‑1 (MQ=255)
                                                                    gaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGaa       >  2:302712‑M1/6‑76 (MQ=255)
                                                                    gaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGaa       <  2:21294‑M1/71‑1 (MQ=255)
                                                                    gaaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGaa       >  1:239813‑M1/6‑76 (MQ=255)
                                                                     aaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAAc      <  2:406794‑M1/72‑1 (MQ=255)
                                                                     aaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAAc      <  2:1391085‑M1/72‑1 (MQ=255)
                                                                     aaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAAc      <  2:1002033‑M1/72‑1 (MQ=255)
                                                                     aaagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAAc      <  2:904417‑M1/72‑1 (MQ=255)
                                                                      aagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAAca     <  1:1228671‑M1/73‑1 (MQ=255)
                                                                      aagGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAAca     >  1:1110441‑M1/4‑76 (MQ=255)
                                                                        gGTCGTTGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAAcagc   >  2:877511‑M1/2‑76 (MQ=255)
                                                                         GTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAAcagca  <  2:1455446‑M1/76‑1 (MQ=255)
                                                                         |                                                                           
CCGGAACGTTTCGATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCA  >  REL606/2904031‑2904179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: