New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 2039999	65 (1.080)	5 (0.090)	5/138	6.7	8.5%	coding (130/438 nt)	vioB	VioB, involved in dTDP‑N‑acetylviosamine synthesis
	?	REL606	= 2040151	48 (0.870)					intergenic (‑23/+120)	vioB/vioA	VioB, involved in dTDP‑N‑acetylviosamine synthesis/VioA, involved in dTDP‑N‑acetylviosamine synthesis
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCTATATGAATATAATCGTGTATATCAATACCACCCTCTCCAGCTGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAATACTAATATTATCACACCCATAAAAAGATGCCTTATCTGATATTTTTACATTTTCTCCAACGCTTT  >  REL606/2040076‑2040226                                                                            |                                                                            gCTATATGAATATAATCGTGTATATCAATACCACCCTCTCCAGCTGAAAACACGCAAAAATCATCAATCCTTACat                                                                             >  1:1250775/1‑76 (MQ=255)   tataTGAATATAATCGTGTATATCAATACCACCCTCTCCAGCTGAAAACACGCAAAAATCATCAATCCTTACatta                                                                           >  1:157210/1‑76 (MQ=255)    atatGAATATAATCGTGTATATCAATACCACCCTCTCCAGCTGAAAACACGCAAAAATCATCAATCCTTACattat                                                                          <  2:1848820/76‑1 (MQ=255)          atatAATCGTGTATATCAATACCACCCTCTCCAGCTGAAAACACGCAAAAATCATCAATCCTTACATTATTccagt                                                                    <  2:1598464/76‑4 (MQ=255)          atatAATCGTGTATATCAATACCACCCTCTCCAGCTGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCaa                                                                    <  1:717113/76‑1 (MQ=255)           tataATCGTGTATATCAATACCACCCTCTCCAGCTGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAAt                                                                   <  1:626045/76‑1 (MQ=255)            ataATCGTGTATATCAATACCACCCTCTCCAGCTGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAATa                                                                  <  2:1554069/76‑1 (MQ=255)            ataATCGTGTATATCAATACCACCCTCTCCAGCTGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAATa                                                                  <  1:552047/76‑1 (MQ=255)            ataATCGTGTATATCAATACCACCCTCTCCAGCTGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAATa                                                                  >  2:145883/1‑76 (MQ=255)            ataATCGTGTATATCAATACCACCCTCTCCAGCTGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAATa                                                                  <  1:984412/76‑1 (MQ=255)             taATCGTGTATATCAATACCACCCTCTCCAGCTGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAATAc                                                                 <  1:1401025/76‑1 (MQ=255)             taATCGTGTATATCAATACCACCCTCTCCAGCTGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAATAc                                                                 <  2:1461736/76‑1 (MQ=255)                tCGTGTATATCAATACCACCCTCTCCAGCTGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAATACTaa                                                              <  2:1466388/76‑1 (MQ=255)                  gtgtATATCAATACCACCCTCTCCAGCTGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAATACTAata                                                            <  1:809273/76‑1 (MQ=255)                          cAATACCACCCTCTCCAGCTGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAATACTAATATTATcaca                                                    <  1:1584715/76‑1 (MQ=255)                          cAATACCACCCTCTCCAGCTGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAATACTAATATTATcaca                                                    <  1:77694/76‑1 (MQ=255)                          cAATACCACCCTCTCCAGCTGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAATACTAATATTATcaca                                                    >  2:716356/1‑76 (MQ=255)                           aaTACCACCCTCTCCAGCTGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAATACTAATATTATCacac                                                   >  2:295553/1‑76 (MQ=255)                             tACCACCCTCTCCAGCTGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAATACTAATATTATCACAccc                                                 >  1:546771/1‑76 (MQ=255)                              accaccCTCTCCAGCTGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAATACTAATATTATCACACCCa                                                >  1:1107164/1‑76 (MQ=255)                              accaccCTCTCCAGCTGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAATACTAATATTATCACACCCa                                                >  1:1107183/1‑76 (MQ=255)                                 accCTCTCCAGCTGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAATACTAATATTATCACACCCATaa                                             <  1:695757/76‑1 (MQ=255)                                 accCTCTCCAGCTGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAATACTAATATTATCACACCCATaa                                             >  2:372696/1‑76 (MQ=255)                                  cccTCTCCAGCTGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAATACTAATATTATCACGCCCATaaa                                            <  2:1556053/76‑1 (MQ=255)                                  cccTCTCCAGCTGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAATACTAATATTATCACACCCATaaa                                            <  1:642841/76‑1 (MQ=255)                                  cccTCTCCAGCTGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAATACTAATATTATCACACCCATaaa                                            <  1:642852/76‑1 (MQ=255)                                   ccTCTCCAGCTGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAATACTAATATTATCACACCCATaaaa                                           <  1:1519890/76‑1 (MQ=255)                                     tctcCAGCTGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAATACTAATATTATCACACCCATAAAAAg                                         <  2:840045/76‑1 (MQ=255)                                      ctcCAGCTGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAATACTAATATTATCACACCCATAAAAAGa                                        <  1:1685596/76‑1 (MQ=255)                                      ctcCAGCTGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAATACTAATATTATCACACCCATAAAAAGa                                        >  1:1651322/1‑76 (MQ=255)                                        ccAGCTGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAATACTAATATTATCACACCCATAAAAAGATg                                      <  1:351184/76‑1 (MQ=255)                                         cAGCTGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAATACTAATATTATCACACCCATAAAAAGATGc                                     <  1:159533/76‑1 (MQ=255)                                         cAGCTGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAATACTAATATTATCACACCCATAAAAAGATGc                                     <  1:1069146/76‑1 (MQ=255)                                          aGCTGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAATACTAATATTATCACACCCATAAAAAGATGcc                                    >  1:1585419/1‑76 (MQ=255)                                           gCTGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAATACTAATATTATCACACCCATAAAAAGATGCCt                                   >  2:1639726/1‑76 (MQ=255)                                             tGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAATACTAATATTATCACACCCATAAAAAGATGCCTTa                                 >  2:1720454/1‑76 (MQ=255)                                              gAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAATACTAATATTATCACACCCATAAAAAGATGCCTTAt                                <  2:776088/76‑1 (MQ=255)                                                   cacGCAAAAATCATCAATCCTTACATTATTCCCAATACTAATATTATCACACCCATAAAAAGATGCCTTATCTGat                           >  2:846990/1‑76 (MQ=255)                                                    acGCAAAAATCATCAATCCTTACATTATTCCCAATACTAATATTATCACACCCATAAAAAGATGCCTTATCTGata                          <  2:1437582/76‑1 (MQ=255)                                                    acGCAAAAATCATCAATCCTTACATTATTCCCAATACTAATATTAACACACCCATAAAAAGATGCCTTATCTGata                          <  2:1437609/76‑1 (MQ=255)                                                         aaaaTCATCAATCCTTACATTATTCCCAATACTAATATTATCACACCCATAAAAAGATGCCTTATCTGATAttttt                     >  2:1027102/1‑76 (MQ=255)                                                             tcatcaATCCTTACATTATTCCCAATACTAATATTATCACACCCATAAAAAGATGCCTTATCTGATATTTTTACAt                 >  2:985848/1‑76 (MQ=255)                                                             tcatcaATCCTTACATTATTCCCAATACTAATATTATCACACCCATAAAAAGATGCCTTATCTGATATTTTTACAt                 >  2:985876/1‑76 (MQ=255)                                                                   aTCCTTACATTATTCCCAATACTAATATTATCACACCCATAAAAAGATGCCTTATCTGATATTTTTACATTTtctc           >  1:691584/1‑76 (MQ=255)                                                                     ccTTACATTATTCCCAATACTAATATTATCACACCCATAAAAAGATGCCTTATCTGATATTTTTACATTTTCTCCa         >  1:1205213/1‑76 (MQ=255)                                                                      cTTACATTATTCCCAATACTAATATTATCACACCCATAAAAAGATGCCCTATCTGATATTTTTACATTTTCTCCaa        <  1:1557809/76‑1 (MQ=255)                                                                        tACATTATTCCCAATACTAATATTATCACACCCATAAAAAGATGCCTTATCTGATATTTTTACATTTTCTCCAACg      <  1:820961/76‑1 (MQ=255)                                                                         aCATTATTCCCAATACTAATATTATCACACCCATAAAAAGATGCCTTATCTGATATTTTTACATTTTCTCCAACGc     >  2:569999/1‑76 (MQ=255)                                                                         aCATTATTCCCAATACTAATATTATCACACCCATAAAAAGATGCCTTATCTGATATTTTTACATTTTCTCCAACGc     >  2:569993/1‑76 (MQ=255)                                                                           attattCCCAATACTAATATTATCACACCCATAAAAAGATGCCTTATCTGATATTTTTACATTTTCTCCAACGCtt   >  2:880285/1‑76 (MQ=255)                                                                            ttattCCCAATACTAATATTATCACACCCATAAAAAGATGCCTTATCTGATATTTTTACATTTTCTCCAACGCttt  >  2:867146/1‑76 (MQ=255)                                                                            ttattCCCAATACTAATATTATCACACCCATAAAAAGATGCCTTATCTGATATTTTTACATTTTCTCCAACGCttt  <  1:1465733/76‑1 (MQ=255)                                                                            |                                                                            GCTATATGAATATAATCGTGTATATCAATACCACCCTCTCCAGCTGAAAACACGCAAAAATCATCAATCCTTACATTATTCCCAATACTAATATTATCACACCCATAAAAAGATGCCTTATCTGATATTTTTACATTTTCTCCAACGCTTT  >  REL606/2040076‑2040226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.