Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	REL606	2,298,775	(GCC)3→2	coding (306‑308/1359 nt)	glpT ←	sn‑glycerol‑3‑phosphate transporter

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	REL606	2298769	2298771	3	45 [0]	[0] 45	glpT	sn‑glycerol‑3‑phosphate transporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	2298772 =	0 (0.000)	43 (0.780)	33/138	0.7	100%	coding (311/1359 nt)	glpT	sn‑glycerol‑3‑phosphate transporter
	?	REL606	= 2298768	0 (0.000)					coding (315/1359 nt)	glpT	sn‑glycerol‑3‑phosphate transporter

CAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAAT  >  REL606/2298694‑2298843                                                                           |                                                                            cAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTg                                                                            >  2:1656172‑M2/1‑75 (MQ=255)  agagGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTgc                                                                           <  2:130346‑M2/76‑3 (MQ=255)       aacaacAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTgccgcca                                                                      >  1:1119712‑M2/1‑69 (MQ=255)          aacaGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTgccgccagaa                                                                   <  2:41457‑M2/76‑11 (MQ=255)             aGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTgccgccagaatca                                                                >  1:1815346‑M2/1‑63 (MQ=255)                 cAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTgccgccagaatcaaacc                                                            >  2:361966‑M2/1‑59 (MQ=255)                  aaaCATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTgccgccagaatcaaacct                                                           >  2:960734‑M2/1‑58 (MQ=255)                   aaCATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTgccgccagaatcaaacctg                                                          <  2:558605‑M2/76‑20 (MQ=255)                   aaCATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAAAAACATCACTgccgccagaatcaaacctg                                                          <  2:553367‑M2/76‑20 (MQ=255)                         aCCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTgccgccagaatcaaacctgcgggca                                                    <  2:44511‑M2/76‑26 (MQ=255)                            gCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTcccgccagaatcaaacctgcgggcagga                                                 <  1:1159426‑M2/76‑29 (MQ=255)                               aTGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTgccgccagaatcaaacctgcgggcaggaaaa                                              <  2:160603‑M2/76‑32 (MQ=255)                               aTGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTgccgccagaatcaaacctgcgggcaggaaaa                                              <  2:527963‑M2/76‑32 (MQ=255)                                tGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTgccgccagaatcaaacctgcgggcaggaaaac                                             <  2:1446060‑M2/76‑33 (MQ=255)                                tGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTgccgccagaatcaaacctgcgggcaggaaaac                                             >  1:130346‑M2/1‑44 (MQ=255)                                  cTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTgccgccagaatcaaacctgcgggcaggaaaacgc                                           <  2:147411‑M2/76‑35 (MQ=255)                                  cTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTgccgccagaatcaaacctgcgggcaggaaaacgc                                           <  1:623864‑M2/76‑35 (MQ=255)                                      gcgTCGCCCATGGCACAAAGCCCATAAACAACATCACTgccgccagaatcaaacctgcgggcaggaaaacgcgcgg                                       >  2:1278385‑M2/2‑38 (MQ=255)                                         tCGCCCATGGCACAAAGCCCATAAACAACATCACTgccgccagaatcaaacccgcgggcaggaaaacgcgcggatt                                    <  1:954991‑M2/76‑42 (MQ=255)                                          cGCCCATGGCACAAAGCCCATAAACAACATCACTgccgccagaatcaaacctgcgggcagtaaaacgcgcggattc                                   >  2:308697‑M2/1‑34 (MQ=255)                                          cGCCCATGGCACAAAGCCCATAAACAACATCACTgccgccagaatcaaacctgcgggcaggaaaacgcgcggattc                                   <  1:678104‑M2/76‑43 (MQ=255)                                          cGCCCATGGCACAAAGCCCATAAACAACATCACTgccgccagaatcaaacctgcgggcaggaaaacgcgcggattc                                   >  1:44511‑M2/1‑34 (MQ=255)                                           gCCCATGGCACAAAGCCCATAAACAACATCACTgccgccagaatcaaacctgcgggcaggaaaacgcgcggattcg                                  <  1:697864‑M2/76‑44 (MQ=255)                                             ccATGGCACAAAGCCCATAAACAACATCACTgccgccagaatcaaacctgcgggcaggaaaacgcgcggattcgag                                >  2:1222887‑M2/1‑31 (MQ=255)                                             ccATGGCACAAAGCCCATAAACAACATCACTgccgccagaatcaaacctgcgggcaggaaaacgcgcggattcgag                                >  2:137467‑M2/1‑31 (MQ=255)                                             ccATGGCACAAAGCCCATAAACAACATCACTgccgccagaatcaaacctgcgggcaggaaaacgcgcggattcgag                                <  1:213068‑M2/76‑46 (MQ=255)                                              cATGGCACAAAGCCCATAAACAACATCACTgccgccagaatcaaacctgcgggcaggaaaacgcgcggattcgagc                               <  1:496732‑M2/76‑47 (MQ=255)                                               aTGGCACAAAGCCCATAAACAACATCACTgccgccagaatcaaacctgcgggcaggaaaacgcgcggattcgagcg                              >  1:1228419‑M2/1‑29 (MQ=255)                                                tGGCACAAAGCCCATAAACAACATCACTgccgccagaatcaaacctgcgggcaggaaaacgcgcggattcgagcga                             <  1:319344‑M2/76‑49 (MQ=255)                                                    acaAAGCCCATAAACAACATCACTgccgccagaatcaaacctgcgggcaggaaaacgcgcggattcgagcgatccg                         >  2:1884140‑M2/1‑24 (MQ=255)                                                     caAAGCCCATAAACAACATCACTgccgccagaatcaaacctgcgggcaggaaaacgcgcggattcgagcgatccga                        <  2:1727745‑M2/76‑54 (MQ=255)                                                       aaGCCCATAAACAACATCACTgccgcaagaatcaaacctgcgggcaggaaaacgcgcggattcgagcgatccgata                      <  1:1371381‑M2/76‑56 (MQ=255)                                                             agaaaaaacaTCCCGgccgccagaatcaacccggcgggcaggaaaacgcgcggattcgagcgatccgataccgaac                >  1:1669870‑M2/3‑15 (MQ=255)                                                             aTAAACAACATCACTgccgccagaatcaaacctgcgggcaggaaaacgcgcggattcgagcgatccgataccgaac                >  1:541620‑M2/1‑15 (MQ=255)                                                              tAAACAACATCACTgccgccagaatcaaacctgcgggcaggaaaacgcgcggattcgagcgatccgataccgaacc               <  2:685248‑M2/76‑63 (MQ=255)                                                                aacaacATCACTgccgccagaatcaaacctgcgggcaggaaaacgcgcggattcgagcgatccgataccgaaccca             <  2:928706‑M2/76‑65 (MQ=255)                                                                aacaacATCACTgccgccagaatcaaacctgcgggcaggaaaacgcgcggattcgagcgatccgataccgaaccca             <  2:119776‑M2/76‑65 (MQ=255)                                                                     caTCACTgccgccagaatcaaacctgcgggcaggaaaacgcgcggattcgagcgatccgataccgaacccatgatg        <  2:1182510‑M2/76‑70 (MQ=255)                                                                     caTCACTgccgccagaatcaaacctgcgggcaggaaaacgcgcggattcgagcgatccgataccgaacccatgatg        <  1:1406203‑M2/76‑70 (MQ=255)                                                                         aCTgccgccagaatcaaacctgcgggcaggaaaacgcgcggattcgagcgatccgataccgaacccatgatgaatt    <  2:1540796‑M2/76‑74 (MQ=255)                                                                         aCTgccgccagaatcaaacctgcgggcaggaaaacgcgcggattcgagcgatccgataccgaacccatgatgaatt    <  1:1018007‑M2/76‑74 (MQ=255)                                                                         aCTgccgccagaatcaaacctgcgggcaggaaaacgcgcggattcgagcgatccgataccgaacccatgatgaatt    <  1:653168‑M2/76‑74 (MQ=255)                                                                         aCTgccgccagaatcaaacctgcgggcaggaaaacgcgcggattcgagcgatccgataccgaacccatgatgaatt    >  1:1112513‑M2/1‑3 (MQ=255)                                                                          cTgccgccagaatcaaacctgcgggcaggaaaacgcgcggattcgagcgatccgataccgaacccatgatgaattt   >  2:1371381‑M2/1‑2 (MQ=255)                                                                           tgccgccagaatcaaacctgcgggcaggaaaacgcgcggattcgagcgatccgataccgaacccatgatgaatttc  <  1:356298‑M2/76‑76 (MQ=255)                                                                           |                                                                            CAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAAT  >  REL606/2298694‑2298843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑