Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	REL606	3,612,965	Δ4 bp	intergenic (‑26/+154)	dctA ← / ← yhjK	C4‑dicarboxylate transport protein/predicted diguanylate cyclase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 3612964	2 (0.040)	69 (1.390)	47/150	0.1	97.2%	intergenic (‑25/+158)	dctA/yhjK	C4‑dicarboxylate transport protein/predicted diguanylate cyclase
	?	REL606	3612969 =	2 (0.040)					intergenic (‑30/+153)	dctA/yhjK	C4‑dicarboxylate transport protein/predicted diguanylate cyclase

ATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/3612890‑3612964 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATT  >  REL606/3612969‑3613043                                                                                                                                                        ATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGT                                                                            <  1:768596/76‑1   CGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTGT                                                                          <  2:873125/76‑1   CGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTGT                                                                          <  2:1474409/76‑1     CTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTGTAA                                                                        >  1:1557052/1‑76       GTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGG                                                                      >  1:153550/1‑76        TCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGT                                                                     >  2:449398/1‑76        TCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGCGTAAGGT                                                                     >  2:449409/1‑76         CAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGGTTTCATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTT                                                                    >  2:1531622/1‑76           GGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTAT                                                                  <  2:23866/76‑1                 GAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTA                                                            >  1:471272/1‑76                    AGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCT                                                         >  1:851762/1‑76                     GTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTG                                                        <  2:634742/76‑1                      TAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGC                                                       >  2:306100/1‑76                       AAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCG                                                      >  1:52676/1‑76                       AAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCG                                                      >  2:781286/1‑76                        AAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGATACCTGCGG                                                     <  2:712360/76‑1                           GCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCT                                                  <  2:1238266/76‑1                            CTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTT                                                 <  2:1266956/76‑1                            CTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTT                                                 <  2:444510/76‑1                             TTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTA                                                >  2:801329/1‑76                             TTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTA                                                >  1:387601/1‑76                             TTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTA                                                >  2:122543/1‑76                              TTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAA                                               >  1:1310899/1‑76                                TAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAA                                             >  1:1499980/1‑76                                 AAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAAT                                            <  1:1548258/76‑1                                 AAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAAT                                            >  1:1374537/1‑76                                  AACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATA                                           <  2:893783/76‑1                                  AACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATA                                           >  1:707215/1‑76                                  AACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATA                                           <  2:731611/76‑1                                   ACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAA                                          >  2:717597/1‑76                                    CAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAAC                                         <  1:615223/76‑1                                            TTTTCATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGAC                                 <  1:787350/76‑1                                             TTTCATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACA                                <  2:580400/76‑1                                             TTTCATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACA                                <  1:1371101/76‑1                                             TTTCATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACA                                <  1:681351/76‑1                                               TCATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAAC                              >  1:1245133/1‑76                                               TCATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAAC                              >  1:130771/1‑76                                                CATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACA                             >  1:31731/1‑76                                                CATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACA                             >  2:1033918/1‑76                                                CATAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACA                             <  1:1429022/76‑1                                                  TAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATC                           <  1:1297403/76‑1                                                  TAGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATC                           >  1:1454898/1‑76                                                   AGGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCA                          <  2:20941/76‑1                                                    GGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCAC                         <  1:934095/76‑1                                                    GGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCAC                         >  2:1235922/1‑76                                                    GGGTGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCAC                         >  1:1547060/1‑76                                                       nGTCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGA                      >  1:865120/2‑76                                                         TCCTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAA                    <  1:405113/76‑1                                                           CTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATG                  >  2:1207044/1‑76                                                           CTTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATG                  <  2:1312921/76‑1                                                            TTTAGTAAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGT                 >  1:1372045/1‑76                                                                  AAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGG           <  1:748852/76‑1                                                                  AAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGG           <  1:1402140/76‑1                                                                  AAAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGG           <  1:840732/76‑1                                                                   AAACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGA          <  1:1142224/76‑1                                                                    AACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGAT         <  1:1453783/76‑1                                                                    AACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGAT         <  2:603821/76‑1                                                                     ACCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATC        <  1:1580206/76‑1                                                                      CCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTTCCTGGATCA       <  2:983621/76‑1                                                                      CCACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCA       <  1:1185236/76‑1                                                                       CACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCAT      <  1:787462/76‑1                                                                       CACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCAT      <  2:841903/76‑1                                                                        ACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATA     <  2:1567177/76‑1                                                                        ACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATA     <  1:931924/76‑1                                                                        ACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATA     <  1:806260/76‑1                                                                        ACAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATA     <  1:64732/76‑1                                                                         CAGTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAA    <  1:1026112/76‑1                                                                           GTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATT  <  2:303415/76‑1                                                                           GTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATT  <  1:1228890/76‑1                                                                                                                                                        ATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/3612890‑3612964 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATT  >  REL606/3612969‑3613043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.