| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| JC | REL606 | 4,097,038 | (AGCAAGGGAGC)1→2 | coding (535/846 nt) | glpF ← | glycerol facilitator |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | REL606 | 4097027 = | 7 (0.130) | 46 (0.890) | 35/148 | 0.5 | 89.4% | coding (546/846 nt) | glpF | glycerol facilitator |
| ? | REL606 | = 4097037 | 4 (0.080) | coding (536/846 nt) | glpF | glycerol facilitator | |||||
GTCACGCGCTGGGTTCATGGCAAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAAT > REL606/4096952‑4097101 | ggttcatggcaaaacccgtcaatgggcccatagatgcgccaatggccgcaatcagtagaccaatcagcaagggagc > 2:834470‑M1/76‑76 (MQ=255)ggttcatggcaaaacccgtcaatgggcccatagatgcgccaatgaccgcaatcagtagaccaatcagcaagggagc > 1:372135‑M1/76‑76 (MQ=255) tCACGCGCTGGGTTCATGGCAAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCa > 1:14266/1‑76 (MQ=255) tCACGCGCTGGGTTCATGGCAAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCa < 2:566929/76‑1 (MQ=255) gttcatggcaaaacccgtcaatgggcccatagatgcgccaatgaccgcaatcagtagaccaatcagcaagggagca > 1:153974‑M1/75‑76 (MQ=255) cgcgCTGGGTTCATGGCAAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCa < 1:644241/76‑1 (MQ=255) atggcaaaacccgtcaatgggcccatagatgcgccaatgaccgcaatcagtagaccaatcagcaagggagcagcaa > 1:165968‑M1/71‑76 (MQ=255) gcaaaacccgtcaatgggcccatagatgcgccaatgaccgcaatcagtagaccaatcagcaagggagcagcaaggg > 1:809763‑M1/68‑76 (MQ=255) gcaaaacccgtcaatgggcccatagatgcgccaatgaccgcaatcagtagaccaatcagcaagggagcagcaaggg > 1:396283‑M1/68‑76 (MQ=255) tGGGTTCATGGCAAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGa > 1:356375/1‑76 (MQ=255) tGGGTTCATGGCAAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGa > 2:474391/1‑76 (MQ=255) tGGGTTCATGGCAAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGa > 2:769404/1‑76 (MQ=255) aaaacccgtcaatgggcccatagatgcgccaatgaccgcaatcagtagaccaatcagcaagggagcagcaagggag < 2:297304‑M1/11‑1 (MQ=255) aaaacccgtcaatgggcccatagatgcgccaatgaccgcaatcagtagaccaatcagcaagggagcagcaagggag > 2:296632‑M1/66‑76 (MQ=255) ggTTCATGGCAAAACCCGTCAATGGGCCCATAGATGCGCCAATGGCCGCAATCAGTAGACCAATCAGCAAGGGAGc > 2:834470‑M2/1‑75 (MQ=255) ggTTCATGGCAAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGc > 1:372135‑M2/1‑75 (MQ=255) gTTCATGGCAAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGca > 1:153974‑M2/1‑74 (MQ=255) cccgtcaatgggcccatagatgcgccaatgaccgcaatcagtagaccaatcagcaagggagCAGCAAGGGAGCCaa > 2:492853‑M1/62‑76 (MQ=255) aTGGCAAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGcagcaa > 1:165968‑M2/1‑70 (MQ=255) tcaatgggcccatagatgcgccaatgaccgcaatcagtagaccaatcagcaagggagCAGCAAGGGAGCCAAAggg < 2:691557‑M1/19‑1 (MQ=255) gCAAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGcagcaaggg > 1:396283‑M2/1‑67 (MQ=255) gCAAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGcagcaaggg > 1:809763‑M2/1‑67 (MQ=255) caatgggcccatagatgcgccaatgaccgcaatcagtagaccaatcagcaagggagCAGCAAGGGAGCCAAAGGGc < 1:667081‑M1/20‑1 (MQ=255) caatgggcccatagatgcgccaatgaccgcaatcagtagaccaatcagcaagggagCAGCAAGGGAGCCAAAGGGc < 1:306751‑M1/20‑1 (MQ=255) caatgggcccatagatgcgccaatgaccgcaatcagtagaccaatcagcaagggagCAGCAAGGGAGCCAAAGGGc < 1:1316749‑M1/20‑1 (MQ=255) atgggcccatagatgcgccaatgaccgcaatcagtagaccaatcagcaagggagCAGCAAGGGAGCCAAAGGGCcg > 1:1235206‑M1/55‑76 (MQ=255) aaaaCCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGcagcaagggag > 2:296632‑M2/1‑65 (MQ=255) aaaaCCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGcagcaagggag < 2:297304‑M2/76‑12 (MQ=255) tgggcccatagatgcgccaatgaccgcaatcagtagaccaatcagcaagggagCAGCAAGGGAGCCAAAGGGccgg < 2:1467059‑M1/23‑2 (MQ=255) tgggcccatagatgcgccaatgaccgcaatcagtagaccaatcagcaagggagCAGCAAGGGAGCCAAAGGGCcgc < 1:604536‑M1/23‑1 (MQ=255) gcccatagatgcgccaatgaccgcaatcagtagaccaatcagcaagggagCAGCAAGGGAGCCAAAGGGCCGCGTg > 2:454301‑M1/51‑76 (MQ=255) cccGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGcagcaagggagccaa > 2:492853‑M2/1‑61 (MQ=255) ccatagatgcgccaatgaccgcaatcagtagaccaatcagcaagggagCAGCAAGGGAGCCAAAGGGCCGCGTGGt < 2:1403681‑M1/28‑1 (MQ=255) catagatgcgccaatgaccgcaatcagtagaccaatcagcaagggagCAGCAAGGGAGCCAAAGGGCCGCGTGGTa < 1:366184‑M1/29‑1 (MQ=255) tCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGcagcaagggagccaaaggg < 2:691557‑M2/76‑20 (MQ=255) atagatgcgccaatgaccgcaatcagtagaccaatcagcaagggagCAGCAAGGGAGCCAAAGGGCCGCGTGGTac < 1:866656‑M1/30‑1 (MQ=255) tagatgcgccaatgaccgcaatcagtagaccaatcagcaagggagCAGCAAGGGAGCCAAAGGGCCGCGTGGTaca > 2:801185‑M1/46‑76 (MQ=255) tagatgcgccaatgaccgcaatcagtagaccaatcagcaagggagCAGCAAGGGAGCCAAAGGGCCGCGTGGTaca < 2:1017050‑M1/31‑1 (MQ=255) tagatgcgccaatgaccgcaatcagtagaccaatcagcaagggagCAGCAAGGGAGCCAAAGGGCCGCGTGGTaca > 2:1562385‑M1/46‑76 (MQ=255) cAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGcagcaagggagccaaagggc < 1:306751‑M2/76‑21 (MQ=255) cAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGcagcaagggagccaaagggc < 1:667081‑M2/76‑21 (MQ=255) cAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGcagcaagggagccaaagggc < 1:1316749‑M2/76‑21 (MQ=255) agatgcgccaatgaccgcaatcagtagaccaatcagcaagggagCAGCAAGGGAGCCAAAGGGCCGCGTGGTacac < 1:703301‑M1/32‑1 (MQ=255) aTGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGcagcaagggagccaaagggccg > 1:1235206‑M2/1‑54 (MQ=255) tGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGcagcaagggagccaaagggccgg < 2:1467059‑M2/76‑24 (MQ=255) tGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGcagcaagggagccaaagggccgc < 1:604536‑M2/76‑24 (MQ=255) gCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGcagcaagggagccaaagggccgcgtg > 2:454301‑M2/1‑50 (MQ=255) ccATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGcagcaagggagccaaagggccgcgtggt < 2:1403681‑M2/76‑29 (MQ=255) cATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGcagcaagggagccaaagggccgcgtggta < 1:366184‑M2/76‑30 (MQ=255) aTAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGcagcaagggagccaaagggccgcgtggtac < 1:866656‑M2/76‑31 (MQ=255) tAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGcagcaagggagccaaagggccgcgtggtaca > 2:1562385‑M2/1‑45 (MQ=255) tAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGcagcaagggagccaaagggccgcgtggtaca > 2:801185‑M2/1‑45 (MQ=255) tAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGcagcaagggagccaaagggccgcgtggtaca < 2:1017050‑M2/76‑32 (MQ=255) atgaccgcaatcagtagaccaatcagcaagggagCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATc < 1:1574801‑M1/42‑1 (MQ=255) tgaccgcaatcagtagaccaatcagcaagggagCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCg > 1:216609‑M1/34‑76 (MQ=255) aGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGcagcaagggagccaaagggccgcgtggtacac < 1:703301‑M2/76‑33 (MQ=255) gaccgcaatcagtagaccaatcagcaagggagCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGt < 1:1373558‑M1/44‑1 (MQ=255) gaccgcaatcagtagaccaatcagcaagggagCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGt < 1:258246‑M1/44‑1 (MQ=255) gaccgcaatcagtagaccaatcagcaagggagCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGt < 2:780864‑M1/44‑1 (MQ=255) atcagtagaccaatcagcaagggagCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTaa > 2:534045‑M1/26‑76 (MQ=255) aaTGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAAc > 1:639946/1‑76 (MQ=255) aTGACCGCAATCAGTAGACCAATCAGCAAGGGAGcagcaagggagccaaagggccgcgtggtacaccgttgccatc < 1:1574801‑M2/76‑43 (MQ=255) tGACCGCAATCAGTAGACCAATCAGCAAGGGAGcagcaagggagccaaagggccgcgtggtacaccgttgccatcg > 1:216609‑M2/1‑33 (MQ=255) gACCGCAATCAGTAGACCAATCAGCAAGGGAGcagcaagggagccaaagggccgcgtggtacaccgttgccatcgt < 1:1373558‑M2/76‑45 (MQ=255) gACCGCAATCAGTAGACCAATCAGCAAGGGAGcagcaagggagccaaagggccgcgtggtacaccgttgccatcgt < 1:258246‑M2/76‑45 (MQ=255) gACCGCAATCAGTAGACCAATCAGCAAGGGAGcagcaagggagccaaagggccgcgtggtacaccgttgccatcgt < 2:780864‑M2/76‑45 (MQ=255) tagaccaatcagcaagggagCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCa > 1:395730‑M1/21‑76 (MQ=255) tagaccaatcagcaagggagCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCa > 1:413378‑M1/21‑76 (MQ=255) agaccaatcagcaagggagCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAg < 1:1363480‑M1/57‑1 (MQ=255) agaccaatcagcaagggagCAGCAAGGGAGCCAAAGGGCCGCGTGGGACACCGCTGCCATCGTCCGCTAACGCCAg < 1:71075‑M1/57‑1 (MQ=255) gaccaatcagcaagggagCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAgg < 2:1545469‑M1/58‑1 (MQ=255) caatcagcaagggagCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATc < 1:1005402‑M1/61‑1 (MQ=255) caatcagcaagggagCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATc > 2:1645228‑M1/16‑76 (MQ=255) aatcagcaagggagCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCa < 1:392311‑M1/62‑1 (MQ=255) aatcagcaagggagCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCa < 2:1045311‑M1/62‑1 (MQ=255) aagcagcaagggagCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCa > 1:995764‑M1/15‑76 (MQ=255) aTCAGTAGACCAATCAGCAAGGGAGcagcaagggagccaaagggccgcgtggtacaccgttgccatcgtccgttaa > 2:534045‑M2/1‑25 (MQ=255) tcagcaagggagCAGCAAGGGAGCCAAAGGGCCGCGTGGAACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGc < 2:198165‑M1/64‑1 (MQ=255) agcaagggagCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGccc > 2:259669‑M1/11‑76 (MQ=255) tAGACCAATCAGCAAGGGAGcagcaagggagccaaagggccgcgtggtacaccgttgccatcgtccgttaacgcca > 1:395730‑M2/1‑20 (MQ=255) tAGACCAATCAGCAAGGGAGcagcaagggagccaaagggccgcgtggtacaccgttgccatcgtccgttaacgcca > 1:413378‑M2/1‑20 (MQ=255) gcaagggagCAGCAAGGGAGCCAAAGGGCCGCGTCGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGcccc > 2:353942‑M1/10‑76 (MQ=255) caagggagCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCa > 1:1347123‑M1/9‑76 (MQ=255) aGACCAATCAGCAAGGGAGcagcaagggagccaaagggccgcgtggtacaccgttgccatcgtccgttaacgccag < 1:1363480‑M2/76‑58 (MQ=255) aGACCAATCAGCAAGGGAGcagcaagggagccaaagggccgcgtgggacaccgctgccatcgtccgctaacgccag < 1:71075‑M2/76‑58 (MQ=255) gACCAATCAGCAAGGGAGcagcaagggagccaaagggccgcgtggtacaccgttgccatcgtccgttaacgccagg < 2:1545469‑M2/76‑59 (MQ=255) aagggagCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCAt < 1:1227006‑M1/69‑1 (MQ=255) agggagCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATc > 1:914552‑M1/7‑76 (MQ=255) gggagCAGCAGGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCa > 2:898035‑M1/6‑76 (MQ=255) gggagCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCa > 2:216514‑M1/6‑76 (MQ=255) cAATCAGCAAGGGAGcagcaagggagccaaagggccgcgtggtacaccgttgccatcgtccgttaacgccaggatc > 2:1645228‑M2/1‑15 (MQ=255) cAATCAGCAAGGGAGcagcaagggagccaaagggccgcgtggtacaccgttgccatcgtccgttaacgccaggatc < 1:1005402‑M2/76‑62 (MQ=255) aaTCAGCAAGGGAGcagcaagggagccaaagggccgcgtggtacaccgttgccatcgtccgttaacgccaggatca < 2:1045311‑M2/76‑63 (MQ=255) aaTCAGCAAGGGAGcagcaagggagccaaagggccgcgtggtacaccgttgccatcgtccgttaacgccaggatca < 1:392311‑M2/76‑63 (MQ=255) aaGCAGCAAGGGAGcagcaagggagccaaagggccgcgtggtacaccgttgccatcgtccgttaacgccaggatca > 1:995764‑M2/1‑14 (MQ=255) tCAGCAAGGGAGcagcaagggagccaaagggccgcgtggaacaccgttgccatcgtccgttaacgccaggatcagc < 2:198165‑M2/76‑65 (MQ=255) | GTCACGCGCTGGGTTCATGGCAAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAAT > REL606/4096952‑4097101 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |