| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| JC JC | REL606 | 3,013,368 | IS150 (–) +3 bp | intergenic (+32/‑50) | ECB_02814 → / → ECB_02815 | conserved hypothetical protein/Capsule polysaccharide export inner‑membrane protein kpsE |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | REL606 | = 2775877 | NA (NA) | 62 (1.220) | 51/150 | 0.1 | 96.8% | noncoding (1/1443 nt) | IS150 | repeat region |
| ? | REL606 | 3013368 = | 2 (0.040) | intergenic (+32/‑52) | ECB_02814/ECB_02815 | conserved hypothetical protein/Capsule polysaccharide export inner‑membrane protein kpsE | |||||
| * | ? | REL606 | = 3013370 | 2 (0.040) | 57 (1.150) | 44/146 | 0.1 | 96.5% | intergenic (+34/‑50) | ECB_02814/ECB_02815 | conserved hypothetical protein/Capsule polysaccharide export inner‑membrane protein kpsE |
| ? | REL606 | = 3894996 | NA (NA) | noncoding (1443/1443 nt) | IS150 | repeat region | |||||
Only 100 of 122 total aligned reads displayed.
AAGGCAAATAAAGTCACTGGCCTTGTGCGCATTTTCGACTAATTAAGCAACGGGGTTCGAGAGGTTAGTCTCTCATTGAGTTTGCGCCCCACTGAAACTTTGATAATCGTTACATGTTGATAAAAGTGAAGTCTGCCGTATCCTGGATGCG > REL606/3013295‑3013445 | tcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaCAt < 1:232425‑M2/3‑1 (MQ=255)tcaaaagggcattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaCAt < 1:1315752‑M2/3‑1 (MQ=255) aGGCAAATAAAGTCACTGGCCTTGTGCGCATTTTCGACTAATTAAGCAACGGGGTTCGAGAGGTTAGTCTCTCATt > 1:517508‑M1/1‑75 (MQ=255) aaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaCATTg < 1:1628966‑M2/5‑1 (MQ=255) gCAAATAAAGTCACTGGCCTTGTGCGCATTTTCGACTAATGAAGCAACGGGGTTCGAGAGGTTAGTCTCTCATtgt < 2:611768‑M1/76‑4 (MQ=255) cAAATAAAGTCACTGGCCTTGTGCGCATTTTCGACTAATTAAGCAACGGGGTTCGAGAGGTTAGTCTCTCATtgta < 2:517508‑M1/76‑5 (MQ=255) cAAATAAAGTCACTGGCCTTGTGCGCATTTTCGACTAATTAAGCAACGGGGTTCGAGAGGTTAGTCTCTCATtgta < 1:653097‑M1/76‑5 (MQ=255) aaaTAAAGTCACTGGCCTTGTGCGCATTTTCGACTAATTAAGCAACGGGGTTCGAGAGGTTAGTCTCTCATtgtac > 2:685671‑M1/1‑71 (MQ=255) ggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaCATTGAGttt > 2:807352‑M2/67‑76 (MQ=255) ggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaCATTGAGttt > 2:944198‑M2/67‑76 (MQ=255) aTAAAGTCACTGGCCTTGTGCGCATTTTCGACTAATTAAGCAACGGGGTTCGAGAGGTTAGTCTCTCATtgtactt > 2:273882‑M1/1‑69 (MQ=255) tAAAGTCACTGGCCTTGTGCGCATTTTCGACTAATTAAGCAACGGGGTTCGAGAGGTTAGTCTCTCATtgtactga > 1:995858‑M1/1‑68 (MQ=255) tattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaCATTGagtttac > 2:1037827‑M2/65‑74 (MQ=255) aaGTCACTGGCCTTGTGCGCATTTTCGACTAATTAAGCAACGGGGTTCGAGAGGTTAGTCTCTCATTGAGTTTgcg > 1:950922/1‑76 (MQ=255) ttttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaCATTGAGTTTgcgc > 1:179677‑M2/63‑76 (MQ=255) ttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaCATTGAGTTTGCGccc > 2:500766‑M2/61‑76 (MQ=255) ggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaCATTGAGTTTGCGCCCCa > 1:1565343‑M2/59‑76 (MQ=255) gctttgacagattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaCATTGAGTTTGCGCCCCAc < 2:1137525‑M2/19‑1 (MQ=255) tGGCCTTGTGCGCATTTTCGACTAATTAAGCAACGGGGTTCGAGAGGTTAGTCTCTCATtgtactgaccccaaaaa < 1:353268‑M1/76‑18 (MQ=255) ctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaCATTGAGTTTGCGCCCCACt > 1:292593‑M2/57‑76 (MQ=255) tttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaCATTGAGTTTGCGCCCCACTg < 1:1028285‑M2/21‑1 (MQ=255) ggCCTTGTGCGCATTTTCGACTAATTAAGCAACGGGGTTCGAGAGGTTAGTCTCTCATtgtactgaccccaaaaag > 1:93378‑M1/1‑58 (MQ=255) ggCCTTGTGCGCATTTTCGACTAATTAAGCAACGGGGTTCGAGAGGTTAGTCTCTCATtgtactgaccccaaaaag > 1:1156091‑M1/1‑58 (MQ=255) ttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaCATTGAGTTTGCGCCCCACTGa > 1:540228‑M2/55‑76 (MQ=255) tgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaCATTGAGTTTGCGCCCCACTGaa < 2:479418‑M2/23‑1 (MQ=255) cTTGTGCGCATTTTCGACTAATTAAGCAACGGGGTTCGAGAGGTTAGTCTCTCATtgtactgaccccaaaaagttg > 2:589403‑M1/1‑55 (MQ=255) cTTGTGCGCATTTTCGACTAATTAAGCAACGGGGTTCGAGAGGTTAGTCTCTCATtgtactgaccccaaaaagttg > 1:1318359‑M1/1‑55 (MQ=255) ttGTGCGCATTTTCGACTAATTAAGCAACGGGGTTCGAGAGGTTAGTCTCTCATtgtactgaccccaaaaagttgg < 2:608454‑M1/76‑23 (MQ=255) ttGTGCGCATTTTCGACTAATTAAGCAACGGGGTTCGAGAGGTTAGTCTCTCATtgtactgaccccaaaaagttgg > 1:740755‑M1/1‑54 (MQ=255) acatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaCATTGAGTTTGCGCCCCACTGAAAc > 1:201169‑M2/52‑76 (MQ=255) acatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaCATTGAGTTTGCGCCCCACTGAAAc < 2:264782‑M2/25‑1 (MQ=255) catattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaCATTGAGTTTGCGCCCCACTGAAACt > 2:927549‑M2/51‑76 (MQ=255) atattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaCATTGAGTTTGCGCCCCACTGAAACtt < 2:368223‑M2/27‑1 (MQ=255) tgCGCATTTTCGACTAATTAAGCAACGGGGTTCGAGAGGTTAGTCTCTCATtgtactgaccccaaaaagttggaca < 1:1233842‑M1/76‑26 (MQ=255) tattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaCATTGAGTTTGCGCCCCACTGAAACttt > 2:902509‑M2/49‑76 (MQ=255) gcgcATTTTCGACTAATTAAGCAACGGGGTTCGAGAGGTTAGTCTCTCATtgtactgaccccaaaaagttggacag < 2:978884‑M1/76‑27 (MQ=255) taggggctattccatttcatcgtccaacaaaatgggtgcagtacaCATTGAGTTTGCGCCCCACTGAAACTTTGAt > 1:441051‑M2/46‑76 (MQ=255) cATTTTCGACTAATTAAGCAACGGGGTTCGAGAGGTTAGTCTCTCATtgtactgaccccaaaaagttggacagtta < 2:1203863‑M1/76‑30 (MQ=255) aggggctattccatttcatcgtccaacaaaatgggtgcagtacaCATTGAGTTTGCGCCCCACTGAAACTTTGATa < 1:1368895‑M2/32‑1 (MQ=255) aggggctattccatttcatcgtccaacaaaatgggtgcagtacaCATTGAGTTTGCGCCCCACTGAAACTTTGATa > 1:220989‑M2/45‑76 (MQ=255) aggggctattccatttcatcgtccaacaaaatgggtgcagtacaCATTGAGTTTGCGCCCCACTGAAACTTTGATa < 2:1251849‑M2/32‑1 (MQ=255) ttttCGACTAATTAAGCAACGGGGTTCGAGAGGTTAGTCTCTCATtgtactgaccccaaaaagttggacagttaaa > 2:631371‑M1/1‑45 (MQ=255) ttttCGACTAATTAAGCAACGGGGTTCGAGAGGTTAGTCTCTCATtgtactgaccccaaaaagttggacagttaaa < 1:774791‑M1/76‑32 (MQ=255) tttCGACTAATTAAGCAACGGGGTTCGAGAGGTTAGTCTCTCATtgtactgaccccaaaaagttggacagttaaac < 1:179643‑M1/76‑33 (MQ=255) ttCGACTAATTAAGCAACGGGGTTCGAGAGGTTAGTCTCTCATtgtactgaccccaaaaagttggacagttaaaca > 2:1305708‑M1/1‑43 (MQ=255) ttCGACTAATTAAGCAACGGGGTTCGAGAGGTTAGTCTCTCATtgtactgaccccaaaaagttggacagttaaaca < 2:113089‑M1/76‑34 (MQ=255) ctattccatttcatcgtccaacaaaatgggtgcagtacaCATTGAGTTTGCGCCCCACTGAAACTTTGATAATCGt > 2:971134‑M2/40‑76 (MQ=255) tattccatttcatcgtccaacaaaatgggtgcagtacaCATTGAGTTTGCGCCCCACTGAAACTTTGATAATCGtt < 1:1260629‑M2/38‑1 (MQ=255) attccatttcatcgtccaacaaaatgggtgcagtacaCATTGAGTTTGCGCCCCACTGGAACTTTGATAATCGTTa > 2:1536285‑M2/38‑76 (MQ=255) attccatttcatcgtccaacaaaatgggtgcagtacaCATTGAGTTTGCGCCCCACTGAAACTTTGATAATCGTTa > 1:859396‑M2/38‑76 (MQ=255) attccatttcatcgtccaacaaaatgggtgcagtacaCATTGAGTTTGCGCCCCACTGAAACTTTGATAATCGTTa > 2:1553265‑M2/38‑76 (MQ=255) attccatttcatcgtccaacaaaatgggtgcagtacaCATTGAGTTTGCGCCCCACTGAAACTTTGATAATCGTTa > 2:1164155‑M2/38‑76 (MQ=255) attccatttcatcgtccaacaaaatgggtgcagtacaCATTGAGTTTGCGCCCCACTGAAACTTTGATAATCGTTa > 1:859387‑M2/38‑76 (MQ=255) ttccatttcatcgtccaacaaaatgggtgcagtacaCATTGAGTTTGCGCCCCACTGAAACTTTGATAATCGTTAc > 1:1137191‑M2/37‑76 (MQ=255) cTAATTAAGCAACGGGGTTCGAGAGGTTAGTCTCTCATtgtactgaccccaaaaagttggacagttaaacacgagg > 2:681506‑M1/1‑38 (MQ=255) catttcatcgtccaacaaaatgggtgcagtacaCATTGAGTTTGCGCCCCACTGAAACTTTGATAATCGTTACATg < 2:1434208‑M2/43‑1 (MQ=255) catgtcatcgtccaacaaaatgggtgcagtacaCATTGAGTTTGCGCCCCACTGAAACTTTGATAATCGTTACATg < 2:1434216‑M2/43‑1 (MQ=255) aTTAAGCAACGGGGTTCGAGAGGTTAGTCTCTCATtgtactgaccccaaaaagttggacagttaaacacgaggcat > 1:1290011‑M1/1‑35 (MQ=255) ttcatcgtccaacaaaatgggtgcagtacaCATTGAGTTTGCGCCCCACTGAAACTTTGATAATCGTTACATGTTg < 2:249646‑M2/46‑1 (MQ=255) tAAGCAACGGGGTTCGAGAGGTTAGTCTCTCATtgtactgaccccaaaaagttggacagttaaacacgaggcatat > 2:328177‑M1/1‑33 (MQ=255) aaGCAACGGGGTTCGAGAGGTTAGTCTCTCATtgtactgaccccaaaaagttggacagttaaacacgaggcatata > 2:413488‑M1/1‑32 (MQ=255) ggcAACGGGGTTCGAGAGGTTAGTCTCTCATtgtactgaccccaaaaagttggacagttaaacacgaggcatatag > 1:853333‑M1/2‑31 (MQ=255) catcgtccaacaaaatgggtgcagtacaCATTGAGTTTGCGCCCCACTGAAACTTTGATAATCGTTACATGTTGAt < 1:780586‑M2/48‑1 (MQ=255) aGCAACGGGGTTCGAGAGGTTAGTCTCTCATtgtactgaccccaaaaagttggacagttaaacacgaggcatatag < 2:68703‑M1/76‑46 (MQ=255) tcgtccaacaaaatgggtgcagtacaCATTGAGTTTGCGCCCCACTGAAACTTTGATAATCGTTACATGTTGATaa < 2:248922‑M2/50‑1 (MQ=255) cacGGGGTTCGAGAGGTTAGTCTCTCATtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtc < 1:1637441‑M1/75‑49 (MQ=255) aCGGGGTTCGAGAGGTTAGTCTCTCATtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtct < 2:86704‑M1/76‑50 (MQ=255) tccaacaaaatgggtgcagtacaCATTGAGTTTGCGCCCCACTGAAACTTTGATAATCGTTACATGTTGATAAAAg > 2:1252644‑M2/24‑76 (MQ=255) cGGGGTTCGAGAGGTTAGTCTCTCATtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctg < 2:1264737‑M1/76‑51 (MQ=255) ccaacaaaatgggtgcagtacaCATTGAGTTTGCGCCCCACTGAAACTTTGATAATCGTTACATGTTGATAAAAGt < 1:169873‑M2/54‑1 (MQ=255) aacaaaatgggtgcagtacaCATTGAGTTTGCGCCCCACTGAAACTTTGATAATCGTTACATGTTGATAAAAGTGa > 2:586653‑M2/21‑76 (MQ=255) ttCGAGAGGTTAGTCTCTCATtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattcc > 2:1575886‑M1/1‑21 (MQ=255) caaaatgggtgcagtacaCATTGAGTTTGCGCCCCACTGAAACTTTGATAATCGTTACATGTTGATAAAAGTGAAg < 1:1459250‑M2/58‑1 (MQ=255) aaaatgggtgcagtacaCATTGAGTTTGCGCCCCACTGAAACTTTGATAATCGTTACATGTTGATAAAAGTGAAGt > 2:1103342‑M2/18‑76 (MQ=255) cGAGAGGTTAGTCTCTCATtgtactgaccccaaaaagttggacagttaaacacgaggcatataggcctgattccga > 2:1129706‑M1/1‑19 (MQ=255) gagaGGTTAGTCTCTCATtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgat < 2:853333‑M1/76‑59 (MQ=255) gagaGGTTAGTCTCTCATtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgat > 2:214582‑M1/1‑18 (MQ=255) gagaGGTTAGTCTCTCATtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgat < 2:1545825‑M1/76‑59 (MQ=255) aatgggtgcagtacaCATTGAGTTTGCGCCCCACTGAAACTTTGATAATCGTTACATGTTGATAAAAGTGAAGTCt > 1:771893‑M2/16‑76 (MQ=255) atgggtgcagtacaCATTGAGTTTGCGCCCCACTGAAACTTTGATAATCGTTACATGTTGATAAAAGTGAAGTCTg < 2:226486‑M2/62‑1 (MQ=255) atgggtgcagtacaCATTGAGTTTGCGCCCCACTGAAACTTTGATAATCGTTACATGTTGATAAAAGTGAAGTCTg < 1:1281338‑M2/62‑1 (MQ=255) tgggtgcagtacaCATTGAGTTTGCGCCCCACTGAAACTTTGATAATCGTTACATGTTGATAAAAGTGAAGTCTGc > 2:779390‑M2/14‑76 (MQ=255) tgggtgcagtacaCATTGAGTTTGCGCCCCACTGAAACTTTGATAATCGTTACATGTTGATAAAAGTGAAGTCTGc < 2:1340088‑M2/63‑1 (MQ=255) gagGTTAGTCTCTCATtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatat > 1:1356653‑M1/1‑16 (MQ=255) ttAGTCTCTCATtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaa > 1:1142594‑M1/1‑12 (MQ=255) tAGTCTCTCATtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaat > 2:50924‑M1/1‑11 (MQ=255) tAGTCTCTCATTGAGTTTGCGCCCCACTGAAACTTTGATAATCGTTACATGTTGATAAAAGTGAAGTCTGCCGTAt > 2:99270/1‑76 (MQ=255) aGTCTCTCATtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaatt < 1:685671‑M1/76‑67 (MQ=255) agtacaCATTGAGTTTGCGCCCCACTGAAACTTTGATAATCGTTACATGTTGATAAAAGTGAAGTCTGCCGTATcc > 1:699588‑M2/7‑76 (MQ=255) tacaCATTGAGTTTGCGCCCCACTGAAACTTTGATAATCGTTACATGTTGATAAAAGTGAAGTCTGCCGTATCCTg < 2:1014501‑M2/72‑1 (MQ=255) tacaCATTGAGTTTGCGCCCCACTGAAACTTTGATAATCGTTACATGTTGATAAAAGTGAAGTCTGCCGTATCCTg < 1:991714‑M2/72‑1 (MQ=255) ctctcATtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattgga < 1:548936‑M1/76‑70 (MQ=255) ctctcATtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattgga > 1:1566556‑M1/1‑7 (MQ=255) acaCATTGAGTTTGCGCCCCACTGAAACTTTGATAATCGTTACATGTTGATAAAAGTGAAGTCTGCCGTATCCTgg < 1:395270‑M2/73‑1 (MQ=255) ctcATtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagt > 1:1273719‑M1/1‑5 (MQ=255) cATtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtca < 1:1122124‑M1/76‑74 (MQ=255) aTtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcag > 1:1487301‑M1/1‑2 (MQ=255) ttgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcaga < 2:1584567‑M1/76‑76 (MQ=255) ttgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcaga < 2:1291488‑M1/76‑76 (MQ=255) ttGAGTTTGCGCCCCACTGAAACTTTGATAATCGTTACATGTTGATAAAAGTGAAGTCTGCCGTATCCTGGATGCg < 1:372283/76‑1 (MQ=255) | AAGGCAAATAAAGTCACTGGCCTTGTGCGCATTTTCGACTAATTAAGCAACGGGGTTCGAGAGGTTAGTCTCTCATTGAGTTTGCGCCCCACTGAAACTTTGATAATCGTTACATGTTGATAAAAGTGAAGTCTGCCGTATCCTGGATGCG > REL606/3013295‑3013445 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |