New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	2041054 =	54 (1.060)	9 (0.200)	5/136	5.1	13.2%	coding (330/1113 nt)	vioA	VioA, involved in dTDP‑N‑acetylviosamine synthesis
	?	REL606	2041468 =	70 (1.520)					coding (1353/1428 nt)	wzx	O antigen translocase (Wzx)
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TTATTTTTTTATAAGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACTTATTTCATTTATAATATAGTT  >  REL606/2041393‑2041536                                                                            |                                                                     ttATTTTTTTATAAGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATg                                                                      <  1:1195168/76‑1 (MQ=255) ttATTTTTTTATAAGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATg                                                                      <  1:425948/76‑1 (MQ=255)  tATTTTTTTATAAGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATgg                                                                     <  1:1486998/76‑1 (MQ=255)    tttttttATAAGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCa                                                                   <  1:936316/76‑1 (MQ=255)        tttATAAGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTta                                                               >  2:523887/1‑76 (MQ=255)        tttATAAGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTta                                                               >  1:1636378/1‑76 (MQ=255)         ttATAAGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTtaa                                                              <  2:82902/76‑1 (MQ=255)         ttATAAGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTtaa                                                              <  1:1594320/76‑1 (MQ=255)            taAGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTtaataa                                                           >  2:796631/1‑76 (MQ=255)            taAGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTtaataa                                                           <  1:162935/76‑1 (MQ=255)            taAGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTtaataa                                                           <  1:419145/76‑1 (MQ=255)            taAGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTtaataa                                                           <  1:73395/76‑1 (MQ=255)             aaGAGCAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATaaa                                                          <  2:1602518/76‑1 (MQ=255)             aaGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATaaa                                                          >  1:841676/1‑76 (MQ=255)             aaGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATaaa                                                          <  1:1140638/76‑1 (MQ=255)             aaGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATaaa                                                          >  1:1567123/1‑76 (MQ=255)             aaGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATaaa                                                          >  1:1567122/1‑76 (MQ=255)             aaGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATaaa                                                          <  1:900270/76‑1 (MQ=255)             aaGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATaaa                                                          <  2:1185148/76‑1 (MQ=255)               gagaAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAAt                                                        <  1:351376/76‑1 (MQ=255)               gagaAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAAt                                                        <  1:1467657/76‑1 (MQ=255)                agaAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGNCATTTAATAAAATa                                                       <  1:885787/76‑1 (MQ=255)                agaAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATa                                                       <  1:655311/76‑1 (MQ=255)                agaAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATa                                                       <  1:308078/76‑1 (MQ=255)                 gaAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAg                                                      <  1:1400799/76‑1 (MQ=255)                 gaAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAg                                                      >  1:1241446/1‑76 (MQ=255)                 gaAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATAAAAAAAAATAg                                                      <  2:788359/76‑1 (MQ=255)                 aaaaTAATAGTAATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAg                                                      <  1:788359/75‑1 (MQ=255)                  aaaTAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGt                                                     >  1:1256194/1‑76 (MQ=255)                   aataatAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTa                                                    <  1:480968/76‑1 (MQ=255)                   aataatAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTa                                                    <  1:695540/76‑1 (MQ=255)                      aataGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAat                                                 <  1:234839/76‑1 (MQ=255)                      aataGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAat                                                 <  1:1341753/76‑1 (MQ=255)                       ataGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAata                                                <  1:1309208/76‑1 (MQ=255)                        taGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAatat                                               <  1:1196556/76‑1 (MQ=255)                        taGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAatat                                               <  1:1196576/76‑1 (MQ=255)                        taGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAatat                                               >  2:92735/1‑76 (MQ=255)                         aGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAatatt                                              >  1:1628558/1‑76 (MQ=255)                           tCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAatattat                                            >  2:441075/1‑76 (MQ=255)                            cATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAatattata                                           <  1:620448/76‑1 (MQ=255)                            cATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAatattata                                           <  1:1334431/76‑1 (MQ=255)                               aaaTATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATAttt                                        <  1:826094/76‑1 (MQ=255)                                aaTATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTta                                       <  1:1144808/76‑1 (MQ=255)                                  tatTTCTTTGTACATAATTCGAATTAGTGTGATGTCTATAATGGCATTTAATAAAATAGTAAATATTATATTtaat                                     >  2:1450739/1‑76 (MQ=255)                                  tatTTCTTTGTACATAATTCGAATTAGTGTGATGTCTATAATGGCATTTAATAAAATAGTAAATATTATATTtaat                                     >  2:1450748/1‑76 (MQ=255)                                  tatTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTtaat                                     >  1:1485077/1‑76 (MQ=255)                                  tatTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTtaat                                     >  1:1485070/1‑76 (MQ=255)                                   atTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTtaata                                    <  1:341806/76‑1 (MQ=255)                                   atTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTtaata                                    <  2:1230308/76‑1 (MQ=255)                                     ttCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATaaa                                  >  2:408778/1‑76 (MQ=255)                                        tttGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGt                               <  1:1202951/76‑1 (MQ=255)                                           gTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTcc                            <  1:1274355/76‑1 (MQ=255)                                            tACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCa                           <  1:1486520/76‑1 (MQ=255)                                             aCATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCAt                          >  1:796354/1‑76 (MQ=255)                                                tAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACt                       <  1:429948/76‑1 (MQ=255)                                                tAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACt                       <  1:429960/76‑1 (MQ=255)                                                tAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACt                       <  1:940085/76‑1 (MQ=255)                                                tAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACt                       <  1:429973/76‑1 (MQ=255)                                                   ttCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACTTAt                    >  2:872714/1‑76 (MQ=255)                                                   ttCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACTTAt                    >  1:345855/1‑76 (MQ=255)                                                    tCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACTTAtt                   <  2:621167/76‑1 (MQ=255)                                                     cGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACTTAttt                  <  1:486260/76‑1 (MQ=255)                                                      gAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACTTATTTc                 <  1:1284198/76‑1 (MQ=255)                                                       aaTTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACTTATTTCa                <  1:1111623/76‑1 (MQ=255)                                                       aaTTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACTTATTTCa                >  1:1033867/1‑76 (MQ=255)                                                           aGTGTGATGTATATAATGGCATGTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACTTATTTCATTta            <  1:329266/76‑1 (MQ=255)                                                             tgtgATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACTTATTTCATTtata          <  2:1232183/76‑1 (MQ=255)                                                                 aTGTATATAATGGCATTTAATAAAATAGTAAATTTTATATTTAATAAGGGTTCCATACTTATTTCATTTATAatat      <  1:995764/76‑1 (MQ=255)                                                                 aTGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACTTATTTCATTTATAatat      <  1:995745/76‑1 (MQ=255)                                                                 aTGGATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACTTATTTCATTTATAatat      <  2:995745/76‑1 (MQ=255)                                                                   gTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACTTATTTCATTTATAATATAg    <  1:1483087/76‑1 (MQ=255)                                                                     atataATAGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACTTATTTCATTTATAATATAGtt  >  1:1053171/1‑76 (MQ=255)                                                                            |                                                                     TTATTTTTTTATAAGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACTTATTTCATTTATAATATAGTT  >  REL606/2041393‑2041536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.