breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsR2_Lane2_BC04_R1.good.fq1,836,964139,474,157100.0%75.9 bases76 bases95.8%
errorsR2_Lane2_BC04_R2.good.fq1,836,963139,474,081100.0%75.9 bases76 bases92.9%
total3,673,927278,948,238100.0%75.9 bases76 bases94.4%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionREL6064,629,81255.72.7100.0%Escherichia coli strain REL606.
total4,629,812100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 1000005751
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 2
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 5000176
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.006

Junction Skew Score Calculation

reference sequencepr(no read start)
REL6060.72026

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 3
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 1

Read Alignment Evidence

optionvalue
ModeConsensus/Mixed Base
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff10
Polymorphism frequency cutoff0.2
Polymorphism minimum variant coverage each strandOFF
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs ofOFF
Skip base substitutions when they create a homopolymer flanked on each side byOFF

Software Versions

programversion
bowtie22.3.4.1
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input04:42:40 02 Feb 201904:43:19 02 Feb 201939 seconds
Read alignment to reference genome04:43:19 02 Feb 201904:49:26 02 Feb 20196 minutes 7 seconds
Preprocessing alignments for candidate junction identification04:49:26 02 Feb 201904:50:08 02 Feb 201942 seconds
Preliminary analysis of coverage distribution04:50:08 02 Feb 201904:51:39 02 Feb 20191 minute 31 seconds
Identifying junction candidates04:51:39 02 Feb 201904:51:40 02 Feb 20191 second
Re-alignment to junction candidates04:51:40 02 Feb 201904:53:00 02 Feb 20191 minute 20 seconds
Resolving best read alignments04:53:00 02 Feb 201904:54:03 02 Feb 20191 minute 3 seconds
Creating BAM files04:54:03 02 Feb 201904:55:27 02 Feb 20191 minute 24 seconds
Tabulating error counts04:55:27 02 Feb 201904:55:50 02 Feb 201923 seconds
Re-calibrating base error rates04:55:50 02 Feb 201904:55:51 02 Feb 20191 second
Examining read alignment evidence04:55:51 02 Feb 201904:59:48 02 Feb 20193 minutes 57 seconds
Polymorphism statistics04:59:48 02 Feb 201904:59:48 02 Feb 20190 seconds
Output04:59:48 02 Feb 201904:59:56 02 Feb 20198 seconds
Total 17 minutes 16 seconds