Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	4,524,522	IS186 (+) +6 bp	coding (494‑499/549 nt)	fimA →	major type 1 subunit fimbrin (pilin)

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	2772854 =	NA (NA)	105 (1.750)	66/144	0.0	100%	noncoding (1/1343 nt)	IS186	repeat region
	?	REL606	= 4524527	0 (0.000)					coding (499/549 nt)	fimA	major type 1 subunit fimbrin (pilin)
*	?	REL606	= 2774196	NA (NA)	71 (1.180)	56/144	0.0	100%	noncoding (1343/1343 nt)	IS186	repeat region
	?	REL606	4524522 =	0 (0.000)					coding (494/549 nt)	fimA	major type 1 subunit fimbrin (pilin)

ACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/2774119‑2774196 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gggCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAG  >  REL606/4524522‑4524592                                                                                                                                                       ACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATG                                                                           <  2:1354215/76‑1  CGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGG                                                                          <  1:680428/76‑1  CGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGG                                                                          <  1:1188785/76‑1  CGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGG                                                                          <  2:1850065/76‑1  CGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGG                                                                          <  2:1890937/76‑1  CGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGG                                                                          >  2:1893151/1‑76  CGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGG                                                                          <  2:869083/76‑1  CGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGG                                                                          <  2:342400/76‑1  CGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGG                                                                          >  2:80521/1‑76   GCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG                                                                         >  2:1635187/1‑76   GCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG                                                                         <  1:73631/76‑1   GCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG                                                                         >  1:47950/1‑76   GCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG                                                                         <  1:1580218/76‑1    CGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGC                                                                        >  2:661029/1‑76    CGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGC                                                                        <  1:84194/76‑1     GAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCC                                                                       >  1:651435/1‑76      AGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCG                                                                      <  1:47745/76‑1      AGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCG                                                                      <  2:156915/76‑1        GCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCA                                                                    >  1:1420671/1‑76         CATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAA                                                                   >  1:158747/1‑76         CATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAA                                                                   <  2:839512/76‑1           TCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACC                                                                 <  2:1395350/76‑1             ggCTTAACGAGGCACCGGGGTGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCC                                                               <  2:1477189/74‑1              TCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCG                                                              <  1:203521/76‑1               CTTAACGAGGCACCGAGGCGTCGGATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGG                                                             >  1:1265034/1‑76               CTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGG                                                             >  2:1384524/1‑76                 TAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGT                                                           <  2:1979397/76‑1                  AACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTG                                                          >  2:364179/1‑76                  AACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTG                                                          >  1:1655488/1‑76                   ACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGC                                                         <  1:1137780/76‑1                      AGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGC                                                      <  1:871883/76‑1                       GGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCT                                                     >  2:1391858/1‑76                         CACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAA                                                   <  2:1956044/76‑1                          agCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGC‑‑CAACCCCGGGTGCTGCTAATGC                                                <  2:536870/74‑1                           CCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATG                                                 <  2:1806300/76‑1                           CCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATG                                                 >  1:1994144/1‑76                           CCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATG                                                 <  2:922847/76‑1                            CGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGC                                                <  2:913966/76‑1                              AGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGG                                              <  1:1023890/76‑1                               GGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGA                                             <  1:1602086/76‑1                                 CGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATG                                           <  1:1032551/76‑1                                  GTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGC                                          >  2:1358360/1‑76                                      tATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACC                                      >  2:968382/2‑76                                      CATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACC                                      <  1:1509677/76‑1                                      CATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACC                                      <  1:656590/76‑1                                       ATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCT                                     <  1:1484561/76‑1                                       ATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCT                                     >  1:1467005/1‑76                                        TTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTT                                    <  2:624972/76‑1                                         TCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGNGGATGCGACCTTC                                   <  1:1340882/76‑1                                          CTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCA                                  >  1:1835550/1‑76                                          CTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCA                                  <  1:1963140/76‑1                                           TTCAGATGGTTCGACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAA                                 <  2:186385/76‑1                                           TTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAA                                 >  1:787540/1‑76                                             CAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGG                               >  2:1095782/1‑76                                             CAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGG                               >  2:1546280/1‑76                                             CAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGG                               >  2:1521452/1‑76                                              AGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGT                              >  1:1970895/1‑76                                               GATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTT                             >  1:757789/1‑76                                               GATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTT                             <  1:1385013/76‑1                                                ATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTC                            <  2:499825/76‑1                                                     TCAGCCCTTAAGTTAGCGCGTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTAT                       <  2:1265034/76‑1                                                     TCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTAT                       >  2:1060146/1‑76                                                      CAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATC                      >  2:1901603/1‑76                                                        ACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAA                    <  2:405548/76‑1                                                          CCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATA                  >  1:1828215/1‑76                                                           CTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAA                 <  1:828587/76‑1                                                           CTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAA                 <  1:332231/76‑1                                                           CTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAA                 >  2:1196547/1‑76                                                           CTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAA                 <  1:1787919/76‑1                                                           CTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAA                 <  1:781581/76‑1                                                            TTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAAC                >  1:690828/1‑76                                                            TTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAAC                >  2:1213564/1‑76                                                              AAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCT              <  2:108743/76‑1                                                               AGTTAGCGCTTATGGGCCGCAACCCCAGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTA             >  1:962330/1‑76                                                                GTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTAC            <  1:1255188/76‑1                                                                  TAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCC          >  1:1616356/1‑76                                                                   AGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCA         <  2:1086267/76‑1                                                                   AGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCA         >  1:986262/1‑76                                                                      GCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGT      <  1:892142/76‑1                                                                       CTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTT     >  2:1410111/1‑76                                                                         TATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCA   >  2:310498/1‑76                                                                         TATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCA   >  1:416014/1‑76                                                                         TATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCA   >  1:614851/1‑76                                                                          ATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAG  >  2:1477570/1‑76                                                                                                                                                       ACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/2774119‑2774196 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gggCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAG  >  REL606/4524522‑4524592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.