Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	REL606	2,298,759	Δ4 bp	coding (321‑324/1359 nt)	glpT ←	sn‑glycerol‑3‑phosphate transporter

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	REL606	2298759	2298762	4	13 [0]	[0] 13	glpT	sn‑glycerol‑3‑phosphate transporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 2298758	0 (0.000)	13 (0.590)	11/150	0.9	100%	coding (325/1359 nt)	glpT	sn‑glycerol‑3‑phosphate transporter
	?	REL606	2298763 =	0 (0.000)					coding (320/1359 nt)	glpT	sn‑glycerol‑3‑phosphate transporter

AACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGA  >  REL606/2298689‑2298830                                                                      |                                                                         aaccGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGCCGCCCATGGCACAAAGCCCATAAAatcact                                                                    >  2:499326‑M1/1‑70 (MQ=255)     gCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAAatcactgccg                                                                <  2:344375‑M1/76‑11 (MQ=255)             acaacaGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAAatcactgccgccgccaga                                                        >  2:324768‑M1/1‑58 (MQ=255)              caacaGTACAAACATCACCGCAATGCTCGACGTCTCCCATGGCACAAAGCCCATAAAatcactgccgccgccagaa                                                       <  2:368750‑M1/76‑20 (MQ=255)                    tACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAAatcactgccgccgccagaatcaaac                                                 >  2:244760‑M1/1‑51 (MQ=255)                      cAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAAatcactgccgccgccagaatcaaacct                                               >  2:564491‑M1/1‑49 (MQ=255)                                        tCGACGTCGCCCATGGCACAAAGCCCATAAAatcactgccgccgccagaagcaaacctgcgggcaggaaaacgctc                             >  2:485765‑M1/1‑31 (MQ=255)                                         cGACGTCGCCCATGGCACAAAGCCCATAAAatcactgccgccgccagaatcaaacctgcgggcaggaaaacgcgcg                            >  2:63711‑M1/1‑30 (MQ=255)                                               cGCCCATGGCACAAAGCCCATAAAatcactgccgccgccagaatcaaacctgcgggcaggaaaacgcgcggcttcg                      >  1:73712‑M1/1‑24 (MQ=255)                                               cGCCCATGGCACAAAGCCCATAAAatcactgccgccgccagaatcaaacctgcgggcaggaaaacgcgcggattcg                      >  2:136968‑M1/1‑24 (MQ=255)                                                    gtgGCACAAAGCCCATAAAatcactgccgccgccagaatcaaacctgcgggcaggaaaacgcgcggattcgagcga                 >  1:397034‑M1/2‑19 (MQ=255)                                                               cccATAAAatcactgccgccgccagaatcaaacctgcgggcaggaaaacgcgcggattcgagcgatccgatacgga      >  1:545007‑M1/1‑8 (MQ=255)                                                                   tAAAatcactgccgccgccagaatcaaacctgcgggcaggaaaacgcgcggattcgagcgatccgataccgaaccc  >  1:597873‑M1/1‑4 (MQ=255)                                                                      |                                                                         AACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGA  >  REL606/2298689‑2298830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.