Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	REL606	3,894,997	Δ6,934 bp	IS150‑mediated	rbsD–[yieO]	rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	REL606	3893609–3894994	3901930	6937–8322	4 [3]	[0] 23	[insJ‑5]–[yieO]	[insJ‑5],insK‑5,rbsD,rbsA,rbsC,rbsB,rbsK,rbsR,[yieO]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 3652533	NA (NA)	23 (1.070)	20/146	0.2	100%	noncoding (1443/1443 nt)	IS150	repeat region
	?	REL606	3901931 =	0 (0.000)					coding (489/1428 nt)	yieO	predicted multidrug or homocysteine efflux system

GGTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAA  >  REL606/3893533‑3893627                                                                            |                    ggTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCaaa                     >  1:119779/1‑76 (MQ=255)     gAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCa                 >  2:454874/1‑76 (MQ=255)          cTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATa            <  1:697859/76‑1 (MQ=255)                    aCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGaa  <  1:582359/76‑1 (MQ=31)                                                                            |                    GGTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAA  >  REL606/3893533‑3893627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑