breseq  version 0.27.0b  revision f32c2f7f32bc
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 1889 shown)
  seq id position ref A a var freq score (cons/poly) reads annotation genes product
*NC_000913417,5330TTG44.7% 210.4 / 64.8 199V131G (GTG→GGG) phoBresponse regulator in two‑component regulatory system with PhoR
*NC_0009132,228,2750TTC44.1% 319.6 / 59.8 237pseudogene (335/1287 nt)mdtQpseudogene; putative channel/filament proteins
*NC_000913743,6570TTG41.2% 260.5 / 25.9 215V22G (GTG→GGG) ybgJputative allophanate hydrolase, subunit 1
*NC_000913433,6490TTG41.2% 266.5 / 82.8 323G65G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*NC_000913283,9400AAG39.6% 155.5 / 14.2 111E247G (GAG→GGG) yagFCP4‑6 prophage; dehydratase family protein
*NC_0009134,622,2500AAG38.6% 501.9 / 127.3 277E161G (GAG→GGG) yjjJputative protein kinase
*NC_0009134,604,3440CCT38.4% 616.9 / 241.1 400S62F (TCT→TTT) bglJbgl operon transcriptional activator
*NC_000913811,7210TTG38.3% 618.7 / 33.9 327V67G (GTG→GGG) bioCmalonyl‑ACP O‑methyltransferase, SAM‑dependent
*NC_0009133,206,4460CCA38.3% 594.7 / 168.5 362intergenic (‑190/‑17)ttdR/ttdAtranscriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
*NC_0009132,228,2720AAC38.1% 433.6 / 48.1 246pseudogene (338/1287 nt)mdtQpseudogene; putative channel/filament proteins
*NC_000913288,3820AAC37.8% 278.7 / 39.6 173T532P (ACC→CCC) yagHCP4‑6 prophage; putative xylosidase/arabinosidase
*NC_0009134,457,1050AAG37.5% 608.1 / 139.2 342I441V (ATC→GTC) mplUDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase
*NC_0009134,101,5070TTG37.5% 981.6 / 65.7 465intergenic (+77/‑183)sodA/kdgTsuperoxide dismutase, Mn/2‑keto‑3‑deoxy‑D‑gluconate transporter
*NC_0009133,401,4590TTC37.5% 586.5 / 109.0 315D625G (GAT→GGT) csrDtargeting factor for csrBC sRNA degradation
*NC_0009132,151,6890GGT37.4% 180.8 / 80.3 212intergenic (+43/+22)yegJ/yegKuncharacterized protein/ser/thr phosphatase‑related protein
*NC_0009133,133,3020TTG37.3% 757.8 / 111.5 376H219P (CAC→CCC) yghSputative ATP‑binding protein
*NC_0009132,326,4500TTG37.1% 680.6 / 127.2 345G114G (GGT→GGGatoBacetyl‑CoA acetyltransferase
*NC_000913273,6760TTG37.0% 987.8 / 77.1 450pseudogene (830/1108 nt)ykfCCP4‑6 prophage; conserved protein;Phage or Prophage Related
*NC_0009132,151,6860GGT37.0% 196.1 / 88.6 212intergenic (+40/+25)yegJ/yegKuncharacterized protein/ser/thr phosphatase‑related protein
*NC_0009132,074,0440TTC36.9% 239.5 / 18.7 123S836P (TCC→CCC) fluCP4‑44 prophage; antigen 43 (Ag43) phase‑variable biofilm formation autotransporter

Marginal new junction evidence (lowest skew 10 of 55 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4542682 =500 (1.210)29 (0.070) 25/308 9.8 6.0% intergenic (+49/‑433) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?NC_000913 4542996 = 440 (1.130)intergenic (+363/‑119) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
* ? NC_000913 1097039 =219 (0.530)64 (0.160)
+C
24/324 10.6 24.0% intergenic (+210/+526) ycdU/serX putative inner membrane protein/tRNA‑Ser
?NC_000913 = 1097580 188 (0.460)noncoding (73/88 nt) serX tRNA‑Ser
* ? NC_000913 = 382590NA (NA)31 (0.080) 24/326 10.7 5.9% noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 564107 = 491 (1.190)coding (374/633 nt) sfmZ response regulator family protein
* ? NC_000913 381260 =NA (NA)27 (0.070) 18/324 12.6 5.1% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 564111 502 (1.220)coding (370/633 nt) sfmZ response regulator family protein
* ? NC_000913 3652036 =NA (NA)100 (0.240) 17/326 13.0 16.3% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 3890078 513 (1.240)coding (1349/1416 nt) tnaA tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent
* ? NC_000913 1097029 =228 (0.550)48 (0.120)
+C
17/324 13.0 19.1% intergenic (+200/+536) ycdU/serX putative inner membrane protein/tRNA‑Ser
?NC_000913 = 1097570 180 (0.440)noncoding (83/88 nt) serX tRNA‑Ser
* ? NC_000913 = 2290113NA (NA)17 (0.040) 16/326 13.4 3.2% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 3890075 = 510 (1.230)coding (1346/1416 nt) tnaA tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent
* ? NC_000913 = 4542690541 (1.310)13 (0.030) 11/308 15.1 2.7% intergenic (+57/‑425) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?NC_000913 = 4542986 440 (1.130)intergenic (+353/‑129) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
* ? NC_000913 = 3815858424 (1.030)10 (0.030) 9/306 16.1 2.8% pseudogene (24/48 nt) rph ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
?NC_000913 3815941 = 290 (0.750)pseudogene (609/669 nt) rph ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
* ? NC_000913 2139570 =291 (0.700)7 (0.020) 7/304 17.2 2.6% intergenic (+85/+189) yegH/asmA inner membrane protein/suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein
?NC_000913 2139603 = 243 (0.630)intergenic (+118/+156) yegH/asmA inner membrane protein/suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein