Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	2,614,614	Δ3 bp	coding (681‑683/849 nt)	focB →	putative formate transporter

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	2614614	2614616	3	47 [0]	[0] 43	focB	putative formate transporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2614613	0 (0.000)	43 (1.100)	34/234	0.2	100%	coding (680/849 nt)	focB	putative formate transporter
	?	NC_000913	2614617 =	0 (0.000)					coding (684/849 nt)	focB	putative formate transporter

GCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCTCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAGTATGTGTTAT  >  NC_000913/2614481‑2614752                                                                                                                                         |                                                                                                                                        attgcgggcgttcgttatgcgacaaaatcgtcgccatgattttgcccatcaccctgtttgtcgccagtggctttgagcactgtatcgccaatttgtttgtgattccgttcgccattgccattcgccatttcgccccccc                                                                                                                                       >  1:63282‑M2/137‑139 (MQ=255)       ggcgttcgttatgcgacaaaatcgtcgccatgattttgcccatcaccctgtttgtcgccagtggctttgagcactgtatcgccaatttgtttgtgattccgttcgccattgccattcgccatttcgccccCCCCTTCTg                                                                                                                                 >  1:50613‑M2/131‑139 (MQ=255)            tcgttatgcgacaaaatcgtcgccatgattttgcccatcaccctgtttgtcgccagtggctttgagcactgtatcgccaatttgtttgtgattccgttcgccattgccattcgccatttcgccccCCCCTTCTGGCAGc                                                                                                                            <  2:50613‑M2/14‑1 (MQ=255)            tcgttatgcgacaaaatcgtcgccatgattttgcccatcaccctgtttgtcgccagtggctttgagcactgtatcgccaatttgtttgtgattccgttcgccattgccattcgccatttcgccccCCCCTTCTGGCAGc                                                                                                                            <  2:203397‑M2/14‑1 (MQ=255)            tcgttatgcgacaaaatcgtcgccatgattttgcccatcaccctgtttgtcgccagtggctttgagcactgtatcgccaatttgtttgtgattccgttcgccattgccattcgccatttcgccccCCCCTTCTGGCAGc                                                                                                                            <  1:75886‑M2/14‑1 (MQ=255)              gttatgcgacaaaatcgtcgccatgattttgcccatcaccctgtttgtcgccagtggctttgagcactgtatcgccaatttgtttgtgattccgttcgccattgccatccgccatttcgccccCCCCTTCTGGCAGCTg                                                                                                                          <  1:79423‑M2/16‑1 (MQ=255)                   gcgacaaaatcgtcgccatgattttgcccatcaccctgtttgtcgccagtggctttgagcactgtatcgccaatttgtttgtgattccgttcgccattgccattcgccatttcgccccCCCCTTCTGGCAGCTGGCGca                                                                                                                     <  2:708043‑M2/21‑1 (MQ=255)                      acaaaatcgtcgccatgattttgcccatcaccctgtttgtcgccagtggctttgagcactgtatcgccaatttgtttgtgattccgttcgccattgccattcgccatttcgccccCCCCTTCTGGCAGCTGGCGCACAg                                                                                                                  >  2:328298‑M2/116‑139 (MQ=255)                      acaaaatcgtcgccatgattttgcccatcaccctgtttgtcgccagtggctttgagcactgtatcgccaatttgtttgtgattccgttcgccattgccattcgccatttcgccccCCCCTTCTGGCAGCTGGCGCACAg                                                                                                                  >  1:340229‑M2/116‑139 (MQ=255)                        aaaatcgtcgccatgattttgcccatcaccctgtttgtcgtcagtggctttgagcactgtatcgccaatttgtttgtgattccgttcgccattgccattcgccatttcgccccCCCCTTCTGGCAGCTGGCGCACAGTa                                                                                                                >  1:684680‑M2/114‑139 (MQ=255)                                        ttttgcccatcaccctgtttgtcgccagtggctttgagcactgtatcgccaatttgtttgtgattccgttcgccattgccattcgccatttcgccccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTcc                                                                                                <  2:726480‑M2/42‑1 (MQ=255)                                            cccatcaccctgtttgtcgccagtggctttgagcactgtatcgccaatttgtttgtgattccgttcgtcattgccattcgccatttcgcccccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCa                                                                                            >  2:500213‑M2/94‑139 (MQ=255)                                                 tcaccctgtttgtcgccagtggctttgagcactgtatcgccaatttgtttgtgattccgttcgccattgccattcgccatttcgccccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGAc                                                                                       >  2:731758‑M2/89‑139 (MQ=255)                                                  caccctgtttgtcgccagtggctttgagcactgtatcgccaatttgtttgtgattccgttcgccattgccattcgccatttcgccccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACg                                                                                      >  1:209782‑M2/88‑139 (MQ=255)                                                        gtttgtcgtcagtggctttgagcactgtatcgccaatttgtttgtgattccgttcgccattgccattcgccatttcgccccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGc                                                                                <  2:684680‑M2/58‑1 (MQ=255)                                                        gtttgtcgccagtggctttgagcactgtatcgccaatttgtttgtgattccgttcgccattgccattcgccatttcgccccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGc                                                                                <  1:302010‑M2/58‑1 (MQ=255)                                                        gtttgtcgccagtggctttgagcactgtatcgccaatttgtttgtgattccgttcgccattgccattcgccatttcgccccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGc                                                                                <  1:125064‑M2/58‑1 (MQ=255)                                                            gtcgccagtggctttgagcactgtatcgccaatttgtttgtgattccgttcgccattgccattcgccatttcgccccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCAtt                                                                            <  1:557411‑M2/62‑1 (MQ=255)                                                               gccagtggctttgagcactgtatcgccaatttgtttgtgattccgttcgccattgccattcgccatttcgccccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGc                                                                                <  1:748020‑M2/58‑1 (MQ=255)                                                               gccagtggctttgagcactgtatcgccaatttgtttgtgattccgttcgccattgccattcgccatttcgccccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGc                                                                                >  2:748020‑M2/75‑132 (MQ=255)                                                                   gtggctttgagcactgtatcgccaatttgtttgtgattccgttcgccattgccattcgccatttcgcccccccc                                                                                                                                      >  2:445652‑M2/71‑74 (MQ=255)                                                                   gtggctttgagcactgtatcgccaatttgtttgggattccgttcgccattgccattcgccatttcgcccccccc                                                                                                                                      <  1:445652‑M2/4‑1 (MQ=255)                                                                          tgagcactgtatcgccaatttgtttgtgattccgttcgccattgccattcgccatttcgccccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAAt                                                              <  2:717680‑M2/76‑1 (MQ=255)                                                                               actgtatcgccaatttgtttgtgattccgttcgccattgccattcgccatttcgccccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCt                                                         <  2:335318‑M2/81‑1 (MQ=255)                                                                                 tgtatcgccaatttgtttgtgattccgttcgccattgccattcgccatttcgccccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCt                                                            >  1:667814‑M2/57‑134 (MQ=255)                                                                                 tgtatcgccaatttgtttgtgattccgttcgccattgccattcgccatttcgccccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCt                                                            <  2:667814‑M2/78‑1 (MQ=255)                                                                                  gtatcgccaatttgtttgtgattccgttcgccattgccattcgccatttcgccccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTccc                                                      >  2:445446‑M2/56‑139 (MQ=255)                                                                                   tatcgccaatttgtttgtgattccgttcgccattgccattcgccatttcgccccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCg                                                     >  2:139338‑M2/55‑139 (MQ=255)                                                                                        ccaatttgtttgtgattccgttcgccattgccattcgccaattcgccccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGAt                                                >  2:234783‑M2/50‑139 (MQ=255)                                                                                         caatttgtttgtgattccgttcgccattgccattcgccatttcgccccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATg                                               <  1:445446‑M2/91‑1 (MQ=255)                                                                                         caatttgtttgtgattccgttagccattgccattcgccatttcgccccCCCCTTCTGGCAGCTGGCGCACAGTAGCACAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATg                                               <  2:469927‑M2/91‑1 (MQ=255)                                                                                           atttgtttgtgattccgttcgccattgccattcgccatttcgccccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCt                                             <  2:594272‑M2/93‑1 (MQ=255)                                                                                             ttgtttgtgattccgttcgccattgccattcgccatttcgccccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTgg                                           >  1:117227‑M2/45‑139 (MQ=255)                                                                                                          cgttcgccattgccattcgccatttcgccccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATcgg                              <  1:731758‑M2/108‑1 (MQ=255)                                                                                                            ttcgccattgccattcgccatttcgccccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATgctgggtaatatcggcggtg                            >  2:780299‑M2/30‑131 (MQ=255)                                                                                                               gccattgccattcgccatttcgccccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCTTTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGgcggtg                         <  2:124600‑M2/113‑1 (MQ=255)                                                                                                                 attgccattcgccatttcgcccccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCggcggtgcc                       <  1:500213‑M2/115‑2 (MQ=255)                                                                                                                    tgccattcgccatttcgccccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGcggtgcggtg                    <  1:354004‑M2/118‑1 (MQ=255)                                                                                                                       cattcgccatttcgccccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTg                 <  1:328298‑M2/121‑1 (MQ=255)                                                                                                                        attcgccatttcgccccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTgg                <  1:139338‑M2/122‑1 (MQ=255)                                                                                                                           cgccatttcgccccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGa             >  2:500297‑M2/15‑139 (MQ=255)                                                                                                                                      cccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAGTATGTGTTAt  <  2:326701‑M2/136‑1 (MQ=255)                                                                                                                                      cccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAGTATGTGTTAt  <  1:500297‑M2/136‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        GCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCTCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAGTATGTGTTAT  >  NC_000913/2614481‑2614752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑