Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 2,614,614 Δ3 bp coding (681‑683/849 nt) focB → putative formate transporter

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 2614614 2614616 3 57 [0] [0] 53 focB putative formate transporter

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 26146130 (0.000)53 (0.940) 45/198 0.1 100% coding (680/849 nt) focB putative formate transporter
?NC_000913 2614617 = 0 (0.000)coding (684/849 nt) focB putative formate transporter

TTATTGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2614476‑2614613
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAGTAT  >  NC_000913/2614617‑2614745
                                                                                                                                                                                                                                                                           
TTATTGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCC                                                                                                                                  >  1:270305/1‑139
         GCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGG                                                                                                                         >  2:522542/1‑139
                       ACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAG                                                                                                           >  1:899143/1‑139
                                            TGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCA                                                                                                              <  1:556051/115‑1
                                            TGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCA                                                                                                              >  2:556051/1‑115
                                                  TCACTCGGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCTGTTCGCCATTGCGATTCGCCATTTCTACCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCGTACAATTTTCCGGCACTGAC                                                                                >  2:206091/1‑139
                                                            TGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCAT                                                                      >  2:903538/1‑139
                                                             GTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATT                                                                     >  1:124703/1‑139
                                                             GTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGT                                                                             >  2:21294/1‑131
                                                             GTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGT                                                                             <  1:21294/131‑1
                                                               CGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGC                                                                                                                     >  2:1191507/1‑89
                                                               CGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGC                                                                                                                     <  1:1191507/89‑1
                                                               CGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCC                                                                                                                                   >  2:1074746/1‑75
                                                               CGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCC                                                                                                                                   <  1:1074746/75‑1
                                                                GCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTA                                                                                                         <  2:643244/100‑1
                                                                GCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTA                                                                                                         >  1:643244/1‑100
                                                                GCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCG                                                                                                                <  2:65141/93‑1
                                                                GCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCG                                                                                                                >  1:65141/1‑93
                                                                    GTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTAC                                                              >  2:1464023/1‑139
                                                                      GGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCG                                                            <  2:899143/139‑1
                                                                       GCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGC                                                           <  2:270305/139‑1
                                                                           TGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAAT                                                       <  1:522542/139‑1
                                                                                 CTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCTTCTG                                                                                                                          <  1:1448288/65‑1
                                                                                 CTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCTTCTG                                                                                                                          >  2:1448288/1‑65
                                                                                  TGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCC                                                >  1:76853/1‑139
                                                                                     ATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGG                                             <  2:76853/139‑1
                                                                                      TCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCC                                                                                                                                   <  1:133020/52‑1
                                                                                      TCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCC                                                                                                                                   >  2:133020/1‑52
                                                                                       CGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTG                                           >  1:1485213/1‑139
                                                                                          CAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATG                                        <  2:124703/139‑1
                                                                                              TTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCG                                                                                                                <  2:1047525/63‑1
                                                                                              TTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCG                                                                                                                >  1:1047525/1‑63
                                                                                              TTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGC                                                                                                                        <  1:210544/55‑1
                                                                                              TTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGC                                                                                                                        >  2:210544/1‑55
                                                                                                GTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGT                                  <  1:1286114/139‑1
                                                                                                     TGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTC                                                                                          <  2:773951/78‑1
                                                                                                     TGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTC                                                                                          >  1:773951/1‑78
                                                                                                       ATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTAT                                                                 <  2:1504949/101‑1
                                                                                                       ATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTAT                                                                 >  1:1504949/1‑101
                                                                                                           CGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTTCGGCACTGACCGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGG                       >  2:684084/1‑139
                                                                                                           CGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATG                                        <  1:979421/122‑1
                                                                                                           CGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATG                                        >  2:979421/1‑122
                                                                                                             TTCGCCATTGCCATTCGCCATTTCGCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGG                    >  1:1165843/1‑139
                                                                                                               CGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCC                                                                                         >  2:1260933/1‑69
                                                                                                               CGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCC                                                                                         <  1:1260933/69‑1
                                                                                                               CGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGC                                                                                                                     >  1:927379/1‑41
                                                                                                               CGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGC                                                                                                                     <  2:927379/41‑1
                                                                                                               CGCCATTGCCATTCGCCATTTCGCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTG                  <  2:1165843/139‑1
                                                                                                                  CATTGCCATTCGCCATTTCGCCCCCCCCTACTGGCAGCTGGCGCACAGTAGCGCAGACAATCTTCCGGCACTGACGGTCAGCCATCTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCG                <  1:206091/139‑1
                                                                                                                     TGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGA                                                                                 <  1:1279573/71‑1
                                                                                                                     TGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGA                                                                                 >  2:1279573/1‑71
                                                                                                                          TTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTGGT        <  1:684084/139‑1
                                                                                                                          TTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTGGT        >  2:736360/1‑139
                                                                                                                            CGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCT                                                     <  1:81020/92‑1
                                                                                                                            CGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCT                                                     >  2:81020/1‑92
                                                                                                                             GCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAG     <  2:1485213/139‑1
                                                                                                                                ATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAGTAT  <  1:903538/139‑1
                                                                                                                                                                                                                                                                           
TTATTGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2614476‑2614613
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAGTAT  >  NC_000913/2614617‑2614745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.