Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,293,006	IS5 (+) +4 bp	intergenic (‑84/‑518)	hns ← / → tdk	global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1293006 =	0 (0.000)	21 (0.390)	18/208	1.6	100%	intergenic (‑84/‑521)	hns/tdk	global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
	?	NC_000913	= 1300693	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1293009	0 (0.000)	57 (1.070)	43/206	0.2	100%	intergenic (‑87/‑518)	hns/tdk	global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
	?	NC_000913	1299499 =	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

TGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGg‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1292873‑1293009 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTT  >  NC_000913/1299499‑1299637                                                                                                                                                                                                                                                                                      TGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGG                                                                                                                                           >  1:1264330/1‑139   CGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAA                                                                                                                                         >  2:901626/1‑139   CGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAATGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAA                                                                                                                                         >  1:1035694/1‑139    GCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAG                                                                                                                                        <  2:602691/139‑1             GTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACA                                                                                                                               <  1:901626/139‑1                  GATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCC                                                                                                                          >  1:1367457/1‑139                      TTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGA                                                                                                                      >  2:426940/1‑139                      TTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGA                                                                                                                      >  1:1299078/1‑139                              AATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGG                                                                                                                                           >  1:1023109/1‑110                              AATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGG                                                                                                                                           <  2:1023109/110‑1                                           TCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATC                                                                                                 <  2:1367457/139‑1                                                   TGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCA                                                                                         <  2:1299078/139‑1                                                          ATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACA                                                                                  <  2:373816/139‑1                                                          ATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACA                                                                                  <  2:1264330/139‑1                                                             TCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAA                                                                                                                                 <  2:563866/89‑1                                                             TCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAA                                                                                                                                 >  1:563866/1‑89                                                                               GGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCC                                                                                                                         >  2:344862/1‑79                                                                               GGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCC                                                                                                                         <  1:344862/79‑1                                                                                GTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAAC                                                            >  2:1051875/1‑139                                                                                GTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGATATGAGA                                                                                                               <  1:8043/88‑1                                                                                GTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGATATGAGA                                                                                                               >  2:8043/1‑88                                                                                     TTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGG                                                                                              <  2:543539/100‑1                                                                                     TTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGG                                                                                              >  1:543539/1‑100                                                                                               AATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACA                                             <  1:1051875/139‑1                                                                                               AATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAG                                                                                            >  2:996748/1‑92                                                                                               AATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAG                                                                                            <  1:996748/92‑1                                                                                                   GAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGA                                         >  2:1257526/1‑139                                                                                                    AGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGA                                                                     <  1:248309/110‑1                                                                                                    AGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGA                                                                     >  2:248309/1‑110                                                                                                    AGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCC                                                                                                                          <  2:1125831/57‑1                                                                                                    AGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCC                                                                                                                          >  1:1125831/1‑57                                                                                                     GCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAAT                                       >  1:1223151/1‑139                                                                                                     GCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAAT                                       >  2:1277892/1‑139                                                                                                      CCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATT                                      >  2:620032/1‑139                                                                                                      CCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAAAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATT                                      >  2:954691/1‑139                                                                                                       CTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGC                                                  >  1:483268/1‑126                                                                                                       CTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGC                                                  <  2:483268/126‑1                                                                                                         TAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAAAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAG                                   <  1:954691/139‑1                                                                                                         TAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGAT                                                                                                                     <  1:888535/57‑1                                                                                                         TAAATTCACTTGTGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGAT                                                                                                                     >  2:888535/1‑57                                                                                                                     TGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCG                       >  1:1346609/1‑139                                                                                                                     TGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCC                                                                                                                          >  1:374506/1‑40                                                                                                                     TGCAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCC                                                                                                                          <  2:374506/40‑1                                                                                                                       CAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCC                                                                                                                          >  2:1264508/1‑38                                                                                                                       CAATAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCC                                                                                                                          <  1:1264508/38‑1                                                                                                                          TAGCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCTGACAGTGAATTCAGCAGTAAGCGCCGTCAGA                  >  1:884123/1‑139                                                                                                                            GCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCTGACAGTGAATTCAGCAGTAAGCGCCGTCAGACC                <  2:884123/139‑1                                                                                                                            GCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACC                <  1:426940/139‑1                                                                                                                            GCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACC                >  2:1311286/1‑139                                                                                                                            GCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGG                                                                               >  2:367025/1‑76                                                                                                                            GCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGG                                                                               <  1:367025/76‑1                                                                                                                            GCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGATATGAG                                                                                                                >  2:607523/1‑43                                                                                                                            GCCAGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGATATGAG                                                                                                                <  1:607523/43‑1                                                                                                                               AGGAATGTAAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGA             >  1:767350/1‑139                                                                                                                                      TAAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGA      >  2:1025641/1‑139                                                                                                                                       AAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGG                                                                               >  1:209451/1‑65                                                                                                                                       AAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGG                                                                               <  2:209451/65‑1                                                                                                                                          GGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTT  >  2:1214957/1‑139                                                                                                                                          GGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAA                                                                                    <  2:443759/57‑1                                                                                                                                          GGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAA                                                                                    >  1:443759/1‑57                                                                                                                                                                                                                                                                                      TGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGg‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1292873‑1293009 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTT  >  NC_000913/1299499‑1299637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.