Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	883,665	IS1 (–) +9 bp		[mdfA]	[mdfA]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	19796 =	NA (NA)	40 (0.750)	34/208	0.4	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	= 883673	0 (0.000)					coding (1/1233 nt)	mdfA	multidrug efflux system protein
*	?	NC_000913	= 279930	NA (NA)	45 (0.860)	33/204	0.4	100%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	883665 =	0 (0.000)					intergenic (‑277/‑8)	ybjG/mdfA	undecaprenyl pyrophosphate phosphatase/multidrug efflux system protein

CGGCAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCG  >  NC_000913/279800‑280065                                                                                                                                   |                                                                                                                                        cGGCAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCgaga                                                                                                                                     >  2:49840/1‑135 (MQ=31) cGGCAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCgaga                                                                                                                                     <  1:49840/135‑1 (MQ=31)    cAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACaaa                                                                                                                              <  2:911044/139‑1 (MQ=37)    cAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACaaa                                                                                                                              <  2:80980/139‑1 (MQ=37)    cAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACaaa                                                                                                                              >  1:41899/1‑139 (MQ=37)         cTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGc                                                                                                                         >  2:55125/1‑139 (MQ=255)                             cccGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCt                                                                                                     >  2:1244615/1‑139 (MQ=255)                               cGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTaa                                                                                                   >  1:724423/1‑139 (MQ=255)                                        cTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATg                                                                                          >  2:1091124/1‑139 (MQ=255)                                          gAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTc                                                                                        <  2:724423/139‑1 (MQ=255)                                          gAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTc                                                                                        <  2:41899/139‑1 (MQ=255)                                           aaCAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCt                                                                                       >  2:916714/1‑139 (MQ=255)                                           aaCAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCt                                                                                       >  2:1104040/1‑139 (MQ=255)                                                gaggGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGccc                                                                                  >  1:1176427/1‑139 (MQ=255)                                                gaggGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGc                                                                                    >  2:1040491/1‑137 (MQ=255)                                                gaggGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGc                                                                                    <  1:1040491/137‑1 (MQ=255)                                                 aggGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCa                                                                                 <  1:1244615/139‑1 (MQ=255)                                                   ggACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGa                                                                                              <  1:443410/124‑1 (MQ=255)                                                   ggACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGa                                                                                              >  2:443410/1‑124 (MQ=255)                                                   ggACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAat                                                                               >  2:1202694/1‑139 (MQ=255)                                                     aCAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGcc                                                                                   >  2:12721/1‑133 (MQ=255)                                                     aCAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGcc                                                                                   <  1:12721/133‑1 (MQ=255)                                                        gCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGAc                                                                                             <  1:552894/120‑1 (MQ=255)                                                        gCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGAc                                                                                             >  2:552894/1‑120 (MQ=255)                                                         cTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTc                                                                         >  1:608020/1‑139 (MQ=255)                                                            aTAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAAc                                                                      <  1:1241571/139‑1 (MQ=255)                                                             tAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACa                                                                     <  1:1091124/139‑1 (MQ=255)                                                             tAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACa                                                                     <  1:342982/139‑1 (MQ=255)                                                             tAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACa                                                                     <  2:690629/139‑1 (MQ=255)                                                             tAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACa                                                                     <  1:55125/139‑1 (MQ=255)                                                                 aaCAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGaa                                                                 <  1:1202694/139‑1 (MQ=255)                                                                   cAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTc                                                                                        >  2:61989/1‑114 (MQ=255)                                                                   cAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTc                                                                                        <  1:61989/114‑1 (MQ=255)                                                                       aGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGAt                                                           <  1:1104040/139‑1 (MQ=255)                                                                           aCTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACt                                                                          <  1:1324951/120‑1 (MQ=255)                                                                           aCTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACt                                                                          >  2:1324951/1‑120 (MQ=255)                                                                              ggAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCtt                                                                                      <  2:1093238/105‑1 (MQ=255)                                                                              ggAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCtt                                                                                      >  1:1093238/1‑105 (MQ=255)                                                                                  cACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTaa                                                                                                                      >  1:1290692/1‑69 (MQ=255)                                                                                  cACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTaa                                                                                                                      <  2:1290692/69‑1 (MQ=255)                                                                                     cTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCa                                             >  1:1042253/1‑139 (MQ=255)                                                                                               ccATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAAc                                                                      <  2:755496/104‑1 (MQ=255)                                                                                               ccATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAAc                                                                      >  1:755496/1‑104 (MQ=255)                                                                                               ccATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAaacaac                                   <  2:608020/139‑1 (MQ=255)                                                                                                  tcatACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCtt                                                                                      >  1:290451/1‑85 (MQ=255)                                                                                                  tcatACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCtt                                                                                      <  2:290451/85‑1 (MQ=255)                                                                                                  tcatACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGa                                <  2:1176427/139‑1 (MQ=255)                                                                                                         cTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGaa                         <  2:1042253/139‑1 (MQ=255)                                                                                                             aTCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACt                                                                          >  1:245803/1‑86 (MQ=255)                                                                                                             aTCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACt                                                                          <  2:245803/86‑1 (MQ=255)                                                                                                                 gTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGc                                                                                    <  1:325687/72‑1 (MQ=255)                                                                                                                 gTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGc                                                                                    >  2:325687/1‑72 (MQ=255)                                                                                                                      ttGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACa                                                                     >  2:1294223/1‑82 (MQ=255)                                                                                                                      ttGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACa                                                                     <  1:1294223/82‑1 (MQ=255)                                                                                                                         gcagcaTCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTc                                                                                                       >  1:199262/1‑45 (MQ=255)                                                                                                                         gcagcaTCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTc                                                                                                       <  2:199262/45‑1 (MQ=255)                                                                                                                          cagcaTCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCaaa        >  1:735863/1‑139 (MQ=38)                                                                                                                            gcaTCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTc                                                                                        <  1:1296696/57‑1 (MQ=255)                                                                                                                            gcaTCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTc                                                                                        >  2:1296696/1‑57 (MQ=255)                                                                                                                            gcaTCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAac      >  2:171670/1‑139 (MQ=37)                                                                                                                                cACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCg  >  1:221861/1‑139 (MQ=18)                                                                                                                                   |                                                                                                                                        CGGCAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCG  >  NC_000913/279800‑280065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.