breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
read file | reads | bases | passed filters | average | longest | mapped | |
---|---|---|---|---|---|---|---|
errors | D-lyx-PAL-A4-F90-I0-R1_S10_L001_R1_001.good.fq | 1,391,565 | 416,855,525 | 100.0% | 299.6 bases | 300 bases | 86.2% |
errors | D-lyx-PAL-A4-F90-I0-R1_S10_L001_R2_001.good.fq | 1,193,226 | 340,674,998 | 100.0% | 285.5 bases | 286 bases | 88.5% |
total | 2,584,791 | 757,530,523 | 100.0% | 293.1 bases | 300 bases | 87.3% |
seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
---|---|---|---|---|---|---|---|
coverage | distribution | NC_000913 | 4,641,652 | 121.2 | 2.3 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
option | limit | actual |
---|---|---|
Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 11714 |
Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 498 |
Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.065 |
reference sequence | pr(no read start) |
---|---|
NC_000913 | 0.81628 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
option | value |
---|---|
Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 0 |
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 6 |
option | value |
---|---|
Mode | Full Polymorphism |
Ploidy | 1 (haploid) |
Consensus mutation E-value cutoff | 10 |
Consensus frequency cutoff | 0.75 |
Consensus minimum variant coverage each strand | OFF |
Consensus minimum total coverage each strand | OFF |
Consensus minimum variant coverage | OFF |
Consensus minimum total coverage | OFF |
Polymorphism E-value cutoff | 2 |
Polymorphism frequency cutoff | 0.05 |
Polymorphism minimum variant coverage each strand | 2 |
Polymorphism minimum total coverage each strand | OFF |
Polymorphism minimum variant coverage | OFF |
Polymorphism minimum total coverage | OFF |
Polymorphism bias cutoff | OFF |
Predict indel polymorphisms | YES |
Skip indel polymorphisms in homopolymers runs of | ≥3 bases |
Skip base substitutions when they create a homopolymer flanked on each side by | ≥5 bases |
program | version |
---|---|
bowtie2 | 2.3.4.1 |
R | 3.4.4 |
step | start | end | elapsed |
---|---|---|---|
Read and reference sequence file input | 11:29:58 27 Mar 2019 | 11:30:52 27 Mar 2019 | 54 seconds |
Read alignment to reference genome | 11:30:52 27 Mar 2019 | 11:46:33 27 Mar 2019 | 15 minutes 41 seconds |
Preprocessing alignments for candidate junction identification | 11:46:33 27 Mar 2019 | 11:47:15 27 Mar 2019 | 42 seconds |
Preliminary analysis of coverage distribution | 11:47:15 27 Mar 2019 | 11:49:37 27 Mar 2019 | 2 minutes 22 seconds |
Identifying junction candidates | 11:49:37 27 Mar 2019 | 11:49:46 27 Mar 2019 | 9 seconds |
Re-alignment to junction candidates | 11:49:46 27 Mar 2019 | 11:54:09 27 Mar 2019 | 4 minutes 23 seconds |
Resolving best read alignments | 11:54:09 27 Mar 2019 | 11:55:27 27 Mar 2019 | 1 minute 18 seconds |
Creating BAM files | 11:55:27 27 Mar 2019 | 11:57:28 27 Mar 2019 | 2 minutes 1 second |
Tabulating error counts | 11:57:28 27 Mar 2019 | 11:58:26 27 Mar 2019 | 58 seconds |
Re-calibrating base error rates | 11:58:26 27 Mar 2019 | 11:58:27 27 Mar 2019 | 1 second |
Examining read alignment evidence | 11:58:27 27 Mar 2019 | 12:56:58 27 Mar 2019 | 58 minutes 31 seconds |
Polymorphism statistics | 12:56:58 27 Mar 2019 | 12:56:59 27 Mar 2019 | 1 second |
Output | 12:56:59 27 Mar 2019 | 12:57:23 27 Mar 2019 | 24 seconds |
Total | 1 hour 27 minutes 25 seconds |