New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	4675357 =	63 (0.680)	10 (0.130)	9/204	NT	22.4%	intergenic (+10/+94)	PP_4137/chrR	outer membrane siderophore receptor/chromate reductase
	?	NC_002947	4675415 =	18 (0.240)					intergenic (+68/+36)	PP_4137/chrR	outer membrane siderophore receptor/chromate reductase

CGACGCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGTTTACCCGCGAATGCGTCATTGAATTTGC‑CGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCG  >  NC_002947/4675266‑4675560                                                                                                                                                       |                                                                                                                                                   cgacgCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGTTTACCCGCGAATGCGTCATTGAATTTGC‑CGACGCATTCGCGGGTAAACCCGCt                                                                                                                                                    <  1:572095/150‑1 (MQ=255) cgacgCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGTTTACCCGCGAATGCGTCATTGAATTTGC‑CGACGCATTCGCGGGTAAACCCGCt                                                                                                                                                    >  1:55698/1‑150 (MQ=255)  gacgCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGTTTACCCGCGAATGCGTCATTGAATTTGC‑CGACGCATTCGCGGGTAAACCCGCta                                                                                                                                                   >  2:1126383/1‑149 (MQ=255)               gcAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGTTTACCCGCGAATGCGTCATTGAATTTGC‑CGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCg                                                                                                                                      <  2:542982/149‑1 (MQ=255)                cAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGTTTACCCGCGAATGCGTCATTGAATTTGC‑CGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGt                                                                                                                                     <  1:711066/150‑1 (MQ=255)                         ttCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGTTTACCCGCGAATGCGTCATTGAATTTGC‑CGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCAcagca                                                                                                                            >  2:2011344/1‑150 (MQ=255)                                  gggCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGTTTACCCGCGAATGCGTCATTGAATTTGC‑CGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAggg                                                                                                                   <  2:55698/150‑1 (MQ=255)                                       aaGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGTTTACCCGCGAATGCGTCATTGAATTTGC‑CGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCa                                                                                                              <  2:285266/150‑1 (MQ=255)                                             ttGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGTTTACCCGCGAATGCGTCATTGAATTTGC‑CGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGc                                                                                                        <  2:1682931/150‑1 (MQ=255)                                              tGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGTTTACCCGCGAATGCGTCATTGAATTTGC‑CGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGcc                                                                                                       <  2:2414614/150‑1 (MQ=255)                                                  gtgtATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGTTTACCCGCGAATGCGTCATTGAATTTGC‑CGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGGTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGt                                                                                                   >  1:438734/1‑150 (MQ=255)                                                   tgtATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGGGGGCGCGGGTTTACCCGCGTATGCGTCATTGAACTTGC‑CGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGt‑t                                                                                                 <  2:985574/150‑1 (MQ=255)                                                          cGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGTTTACCCGCGAATGCGTCATTGAATTTGC‑CGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTc                                                                                          >  1:90206/1‑150 (MQ=255)                                                             ggTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGTTTACCCGCGAATGCGTCATTGAATTTGC‑CGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCAcc                                                                                       <  2:914073/150‑1 (MQ=255)                                                             ggTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGTTTACCCGCGAATGCGTCATTGAATTTGC‑CGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCAcc                                                                                       <  1:2011344/150‑1 (MQ=255)                                                                ttCCTACAGCTTCCGTGGACGTGTGGGCGCGGGTTTACCCGCGAATGCGTCA‑GACGTGTGCTCTTCCGATCTTGTTATAAACCCGCTCCGACACACGGGAGTTGCCCAGCAGGATTAGTGGTTCAGACCGCCCTGT‑TCAACTTCACCCAt                                                                                    <  1:1126383/150‑1 (MQ=255)                                                                   cTACAGCTTCCGCTGACGGGTGGGCGCGGGTTTACCCGCGAATGCGTCATTGAATTTGC‑CGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCACCCATGAc                                                                                 <  1:226268/150‑1 (MQ=255)                                                                             cGCTTACGGGTGGGCGCTGGTTTACCCGCGAATGCTTCATTTAATTTGC‑CGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCACTCATGACGCAAACCTGt                                                                       <  2:438734/150‑1 (MQ=38)                                                                                tGACGGGTGGGCGCGGGTTTACCCGCGAATGCGTCATTGAATTTGC‑CGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCACCCATGACGCAAACCTGTCGa                                                                    >  1:592135/1‑150 (MQ=255)                                                                                            GCGGGTTTACCCGCGAATGCGGCATTGAATTTGC‑CGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGt‑t                                                                                                 <  2:317880‑M1/109‑1 (MQ=255)                                                                                            GCGGGTTTACCCGCGAATGCGGCATTGAATTTGC‑CGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGt‑t                                                                                                 >  1:317880‑M1/1‑109 (MQ=255)                                                                                               GGTTTACCCGCGAATGCGTCGGCAAATTCAA‑TGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAAGTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGa                                                     <  2:1486197‑M1/150‑1 (MQ=255)                                                                                                GTTTACCCGCGAATGCGTCATTGAATTTGC‑CGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCACCCAt                                                                                    >  2:912583‑M1/1‑118 (MQ=255)                                                                                                GTTTACCCGCGAATGCGTCATTGAATTTGC‑CGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCACCCAt                                                                                    <  1:912583‑M1/118‑1 (MQ=255)                                                                                                     acccgcgaatgcgtcggcaaattcaatgacgcattcgcgggtaaacccgcTCCTACACAGGGGCGTTGCACAGCAGGATTAgggg                                                                                                                  >  2:1572529‑M2/51‑85 (MQ=255)                                                                                                     acccgcgaatgcgtcggcaaattcaatgacgcattcgcgggtaaacccgcTCCTACACAGGGGCGTTGCACAGCAGGATTAgggg                                                                                                                  <  1:1572529‑M2/35‑1 (MQ=255)                                                                                                      CCGCGAATGCGTCATTGAATTTGC‑CGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGca                                                                                                                                 >  1:2404861‑M1/1‑68 (MQ=255)                                                                                                      CCGCGAATGCGTCATTGAATTTGC‑CGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGca                                                                                                                                 <  2:2404861‑M1/68‑1 (MQ=255)                                                                                                      CCGCGAATGCGTCATTGAATTTGC‑CGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAAc                                                   <  1:2126879‑M1/145‑1 (MQ=255)                                                                                                      CCGCGAATGCGTCATTGAATTTGC‑CGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAAc                                                   >  2:2126879‑M1/1‑145 (MQ=255)                                                                                                      CCGCGAATGCGTCATTGAATTTGC‑CGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGt                                                <  1:1941123‑M1/148‑1 (MQ=255)                                                                                                      CCGCGAATGCGTCATTGAATTTGC‑CGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGt                                                >  2:1941123‑M1/1‑148 (MQ=255)                                                                                                       ctgcgaatgcgtcggcaaattcaatgacgcattcgcgggtaaacccgcTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTcg                                              >  1:1512302‑M2/49‑150 (MQ=255)                                                                                                       ccgngaatgcgtcggcaaattcaatgacgcattcgcgggtaaacccgcTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTcg                                              >  2:1500226‑M2/49‑150 (MQ=255)                                                                                                       ccgcgaatgcgtcggcaaattcaatgacgcattcgcgggtaaacccgcTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGG‑TCAACTTCACCCATGACGCAAACTTGTCGACAAACGCCTGCAAGAACGGTcg                                              >  1:603798‑M2/49‑150 (MQ=255)                                                                                                        cgcgaatgcgtcggcaaattcaatgacgcattcgcgggtaaacccgcTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑ACAACTGCACCCAAGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTcgc                                             >  2:521611‑M2/48‑150 (MQ=255)                                                                                                           gaatgcgtcggcaaattcaatgacgcattcgcgggtaaacccgcTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTcgc                                             >  2:1757354‑M2/45‑147 (MQ=255)                                                                                                           gaatgcgtcggcaaattcaatgacgcattcgcgggtaaacccgcTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTcgc                                             <  1:1757354‑M2/103‑1 (MQ=255)                                                                                                           gaatgcgtcggcaaattcaatgacgcattcgcgggtaaacccgcTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGNGTc                                          <  2:1512302‑M2/106‑1 (MQ=255)                                                                                                            aTGCGTCATTGAATTTGC‑CGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGAGTAGGGGTT‑AGCTGGTGGGCTAAAAACAGTTCGTATATCGCAAACCTGTCGACAAACGCCTGCAAGAACGGGCGCGTCtt                                        >  1:1474938/1‑150 (MQ=16)                                                                                                             TGCGTCATTGAATTTGC‑CGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTg                                       >  2:90206‑M1/1‑150 (MQ=255)                                                                                                                GTCATTGAATTTGC‑CGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCa                                    <  1:228774‑M1/150‑1 (MQ=255)                                                                                                                   aTTGAATTTGC‑CGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTg                                 >  1:1893101/1‑150 (MQ=255)                                                                                                                     gcaaattcaatgacgcattcgcgggtaaacccgcTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTATGTCTGCCGTGT‑TCATTGCCACTCGTGTCGTAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGa                                <  1:1486197‑M2/116‑1 (MQ=255)                                                                                                                               cGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGa                      >  1:2189065/1‑150 (MQ=255)                                                                                                                                 acgcattcgcgggtaaacccgcTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAAt                    >  2:51715‑M2/23‑150 (MQ=255)                                                                                                                                    cattcgcgggtaaacccgcTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGc                                                         <  1:1586614‑M2/91‑1 (MQ=255)                                                                                                                                    cattcgcgggtaaacccgcTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGc                                                         >  2:1586614‑M2/20‑110 (MQ=255)                                                                                                                                            ggtaaacccgcTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTg                           <  2:1925980‑M2/121‑1 (MQ=255)                                                                                                                                            ggtaaacccgcTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTg                           >  1:1925980‑M2/12‑132 (MQ=255)                                                                                                                                            ggtaaacccgcTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAg         <  2:2189065‑M2/139‑1 (MQ=255)                                                                                                                                             gtaaacccgcTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGc        <  1:51715‑M2/140‑1 (MQ=255)                                                                                                                                                aacccgcTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGcc                                                                                                       <  2:1959367‑M2/46‑1 (MQ=255)                                                                                                                                                aacccgcTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGcc                                                                                                       >  1:1959367‑M2/8‑53 (MQ=255)                                                                                                                                                  cccgcTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCGCTGT‑TCAACTTCGCCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTAAGCTTGCCCGAAGCGTCGAACAAGCTcgcc   >  2:1323227‑M2/6‑150 (MQ=255)                                                                                                                                                  cccgcTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTcgc                                             <  2:1417199‑M2/103‑1 (MQ=255)                                                                                                                                                  cccgcTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTcgc                                             >  1:1417199‑M2/6‑108 (MQ=255)                                                                                                                                                   ccgcTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGa                                <  1:2075447‑M2/116‑1 (MQ=255)                                                                                                                                                   ccgcTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGa                                >  2:2075447‑M2/5‑120 (MQ=255)                                                                                                                                                   ccgcTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTcgccg  >  1:2317955‑M2/5‑150 (MQ=255)                                                                                                                                                       |                                                                                                                                                   CGACGCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGTTTACCCGCGAATGCGTCATTGAATTTGC‑CGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGT‑TCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCG  >  NC_002947/4675266‑4675560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.