New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	1176938 =	53 (1.220)	3 (0.070)	3/238	NT	5.8%	coding (1363/1470 nt)	guaB	IMP dehydrogenase and single strand DNA binding factor
	?	NC_002947	1176967 =	48 (1.180)					coding (1392/1470 nt)	guaB	IMP dehydrogenase and single strand DNA binding factor

CCTCGGCCGGCGCCGAGAAGCTGGTACCGGAAGGCATCGAAGGCCGCGTTCCTTACAAAGGCGCCCTGGCCGCGATCATCCACCAGCTGATGGGCGGCCTGCGTTCGTCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCACGTGCACGACGTGCAAATCACCAAAGAAGCCCCTAACTACCGCGTAGGCTGAGGCTTCCAGCTACAACATAAAGCGGGGCTGTCATGACAG  >  NC_002947/1176795‑1177084                                                                                                                                                |                                                                                                                                                   agtggaaatcggaaacgacgcggggctgtcatgacagccccgctttatgttgtagctggaagcctcagcctacgccggagttaggggcttcttggtggatgtgcacgtcgtgcacgtgggactcggccatgccggcaccggtgATGCGCa                                                                                                                                              >  1:689429‑M1/144‑150 (MQ=255)  ctcGGCCGGCGCCGAGAAGCTGGTACCGGAAGGCATCGAAGGCCGCGTTCCTTACAAAGGCGCCCTGGCCTCGATCATCCACCAGCTGATGGGCGGCCTGCGTTCGTCCACGGTTAACACAGGCAGCGCAACCATCGAAGAAATGCGCAc                                                                                                                                             >  1:336208/1‑150 (MQ=255)  ctcGGCCGGCGCCGAGAAGCTGGTACCGGAAGGCATCGAAGGCCGCGTTCCTTACAAAGGCGCCCTGGCCGCGATCATCCACCAGCTGATGGGCGGCCTGCGTTCGTCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCAc                                                                                                                                             >  1:971444/1‑150 (MQ=255)   tcGGCCGGCGCCGAGAAGCTGGTACCGGAAGGCATCGAAGGCCGCGTTCCTTACAAAGGCGCCCTGGCCGCGATCATCCACCAGCTGATGGGCGGCCTGCGTTCGTCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCAcc                                                                                                                                            >  2:732992/1‑150 (MQ=255)                  aaGCTGGTACCGGAAGGCATCGAAGGCCGCGTTCCTTACAAAGGCGCCCTGGCCGCGATCATCCACCAGCTGATGGGCGGCCTGCGTTTGTCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTg                                                                                                                             <  1:835819/150‑1 (MQ=255)                  aaGCTGGTACCGGAAGGCATCGAAGGCCGCGTTCCTTACAAAGGCGCCCTGGCCGCGATCATCCACCAGCTGATGGGCGGCCTGCGTTCGTCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTg                                                                                                                             <  1:695637/150‑1 (MQ=255)                   aGCTGGTACCGGAAGGCATCGAAGGCCGCGTTCCTTACAAAGGCGCCCTGGCCGCGATCATCCACCAGCTGATGGGCGGCCTGCGTTCGTCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTgc                                                                                                                            <  1:978134/150‑1 (MQ=255)                      tGGTACCGGAAGGCATCGAAGGCCGCGTTCCTTACAAAGGCGCCCTGGCCGCGATAATCCACCAGCTGATGGGCGGCCTGCGTTCGTCCATGGGCTACACCGGCCGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTggcca                                                                                                                         >  2:136873/1‑146 (MQ=255)                          aCCGGAAGGCATCGAAGGCCGCGTTCCTTACAAAGGCGCCCTGGCCGCGATCATCCACCAGCTGATGGGCGGCCTGCGTTCGTCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCAc                                                                                                                     <  1:889810/150‑1 (MQ=255)                          aCCGGAAGGCATCGAAGGCCGCGTTCCTTACAAAGGCGCCCTGGCCGCGATCATCCACCAGCTGATGGGCGGCCTGCGTTCGTCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCAc                                                                                                                     <  1:761802/150‑1 (MQ=255)                             ggAAGGCATCGAAGGCCGCGTTCCTTACAAAGGCGCCCTGGCCGCGATCATCCACCAGCTGATGGGCGGCCTGCGTTCGTCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCgg                                                                                                                  <  1:1001326/150‑1 (MQ=255)                                    aTCGAAGGCCGCGTTCCTTACAAAGGCGCCCTGGCCGCGATCATCCACCAGCTGATGGGCGGCCTGCGTTCGTCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGc                                                                                                           >  2:1033502/1‑150 (MQ=255)                                           gCCGCGTTCCTTACAAAGGCGCCCTGGCCGCGATCATCCACCAGCTGATGGGCGGCCTGCGTTCGTCCATGGGCTACAACGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCg                                                                                                    >  1:747354/1‑150 (MQ=255)                                            ccGCGTTCCTTACAAAGGCGCCCTGGCCGCGATCATCCACCAGCTGATGGGCGGCCTGCGTTCGTCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGa                                                                                                   <  1:380956/150‑1 (MQ=255)                                                 ttCCTTACAAAGGCGCCCTGGCCGCGATCATCCACCAGCTGATGGGCGGTCTGGGTTCGTCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTccc                                                                                              >  1:630852/1‑150 (MQ=255)                                                     ttACAAAGGCGCCCTGGCCGCGATCATCCACCAGCTGATGGGCGGCCTGCGTTCGTCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGCGTCCCACGt                                                                                          >  2:348487/1‑150 (MQ=255)                                                     ttACAAAGGCGCCCTGGCCGCGATCATCCACCAGCTGATGGGCGGCCTGCGTTCGTCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCAGGCATGGCCGAGTCCCGCGt                                                                                          >  2:793699/1‑150 (MQ=255)                                                         aaaGGCGCCCTGGCCGCGATCATCCACCAGCTGATGGGCGGCCTACGTTCGTTCATTGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCACGTGCac                                                                                      <  2:476759/150‑1 (MQ=255)                                                                cccTGGCCGCGATCATCCACCAGCTGATGGGCGGCCTGCGTTCGTCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCACGTGCACGACGTGc                                                                               <  2:135435/150‑1 (MQ=255)                                                                cccTGGCCGCGATCATCCACCAGCTGATGGGCGGCCTGCGTTCGTCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATACTCGGTGCCGGCATGGCCGAGTCCCACGTGCACGACGTGc                                                                               >  1:560764/1‑150 (MQ=255)                                                                   tGGCCGCGATCATCCACCAGCTGATGGGCGGCCTGCGTTCGTCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCACGTGCACGACGTGCaaa                                                                            >  1:162793/1‑150 (MQ=255)                                                                     gCCGCGATCATCCACCAGCTGATGGGCGGCCTGCGTTCGTCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCACGTGCACGTCGTGCacgtg                                                                          <  2:532171/150‑5 (MQ=255)                                                                     gCCGCGATCATCCACCAGCTGATGGGCGGCCTGCGTTCGTCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCACGTGCACGACGTGCAAATc                                                                          <  2:162793/150‑1 (MQ=255)                                                                        gcgATCATCCACCAGCTGATGGGCGGCCTGCGTTCGTCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCAc                                                                                            <  2:37223/129‑1 (MQ=255)                                                                        gcgATCATCCACCAGCTGATGGGCGGCCTGCGTTCGTCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCAc                                                                                            >  1:37223/1‑129 (MQ=255)                                                                          gATCATCCACCAGCTGATGGGCGGCCTGCGTTCGTCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCACGTGCACGACGTGCAAATCACCaa                                                                     >  1:355171/1‑150 (MQ=255)                                                                          gATCATCCACCAGCTGATGGGCGGCCTGCGTTCGTCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCACGTGCACGACGTGCAAATCACCaa                                                                     >  1:355146/1‑150 (MQ=255)                                                                               tcCACCAGCTGATGGGCGGCCTGCGTTCGTCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCACGTGCACGACGTGCAAATCACCAaagaag                                                                >  2:148850/1‑150 (MQ=255)                                                                                ccaccaGCTGATGGGCGGCCTGCGTTCGTCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCACGTGCACGACGTGCAAATCACCAAAGAAGc                                                               <  1:732992/150‑1 (MQ=255)                                                                                  accaGCTGATGGGCGGCCTGCGTTCGTCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCACGTGCACGACGTGCAAATCACCAAAGAAGccc                                                             >  1:490789/1‑150 (MQ=255)                                                                                  accaGCTGATGGGCGGCCTGCGTTCGTCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCACGTGCACGACGTGCAAATCACCAAAGAAGccc                                                             <  1:654358/150‑1 (MQ=255)                                                                                   ccaGCTGATGGGCGGCCTGCGTTCGTCCATGGGCTAAACCTGTAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCACGTGCACGACGTGCAAATCACCAAAGAAGcccc                                                            <  1:11224/150‑1 (MQ=255)                                                                                              gcggcCTGCGTTCGTCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCACGTGCACGACGTGCAAATCACCAAAGAAGCCCCTAACTACcgcg                                                 >  1:903675/1‑150 (MQ=255)                                                                                                    tGCGTTCGTCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCACGTGCACGACGTGCAAATCACCAAAGAAGCCCCTAACTACCGCGTAGGCt                                           >  2:875327/1‑150 (MQ=255)                                                                                                    tGCGTTCGTCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCACGTGCACGACGTGCAAATCACCAAAGAAGCCCCTAACTACCGCGTAGGCt                                           >  1:509869/1‑150 (MQ=255)                                                                                                       gcacgtcgtgcacgtgggactcggccatgccggcaccggtgATGCGCACCAAGCCGGAGTTCGTgcgc                                                                                                                          <  1:951786‑M1/27‑1 (MQ=255)                                                                                                       gcacgtcgtgcacgtgggactcggccatgccggcaccggtgATGCGCACCAAGCCGGAGTTCGTgcgc                                                                                                                          >  2:951786‑M1/42‑68 (MQ=255)                                                                                                        ttCGTCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCACGTGCACGACGTGCAAATCACCAAAGAAGCCCCTAACTACCGCGTAGGCTGAgg                                       >  2:257449/1‑150 (MQ=255)                                                                                                            tCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCACGTGCACGACGTGCAAATCACCAAAGAAGCCCCTAACTACCGCGTAGGCTGAGGCTTc                                   >  2:315734/1‑150 (MQ=255)                                                                                                            tCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCAACGTGCACGACGTGCAAATCACCAAAGAAGCCCCTAACTACCGCGTAGGCTGAGGCTTc                                   >  1:74370/1‑150 (MQ=255)                                                                                                             ccATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCACGTGCACGACGTGCAAATCACCAAAGAAGCCCCTAACTACCGCGTAGGCTGAGGCTTcc                                  <  2:955906/150‑1 (MQ=255)                                                                                                               aTGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCACGTGCACGACGTGCAAATCACCAAAGAAGCCACTAACTACCGCGTAGGCTGAGGCTTCCAg                                >  2:782442/1‑150 (MQ=255)                                                                                                                 gggCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCACGTGCACGACGTGCAAATCACCAAAGAAGCCCCTAACTACCGCGTAGGCTGAGGCTTCCAGCt                              <  1:875327/150‑1 (MQ=255)                                                                                                                     tACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGAACATGACCGGTGCCGGCATGGCCGAGTCCCACGTGCACGACGTGCAAATCACCAAAGAAGCCCCTAACTACCGCGTAGGCTGAGGCTTCCAGCTacaa                          >  2:1122378/1‑150 (MQ=255)                                                                                                                        acCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCACGTGCACGACGTGCAAATCACCAAAGAAGCCCCTACCTACCGCGTAGGCTGAGGCTTCCAGCTACAACAt                       >  1:175508/1‑150 (MQ=255)                                                                                                                        acCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCACGTGCACGACGTGCAAATCACCAAAGAAGCCCCTAACTACCGCGTAGGCTGAGGCTTCCAGCTACAACAt                       <  2:630852/150‑1 (MQ=255)                                                                                                                         ccGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGCGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCACGTGCACGACGTGCAAATCACCAAAGAAGCCCCTAACTACCGCGTAGGCTGAGGCTTCCAGCTACAACATa                      >  2:70003/1‑150 (MQ=255)                                                                                                                               gcgcAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCACGTGCACGACGTGCAAATCACCAAAGAAGCCCCTAACTACCGCGTATGCTGAGGCTTCCAGCTACAACATAAAGCgg                >  2:595705/1‑150 (MQ=255)                                                                                                                               gcgcAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCAACGTGCACGACGTGCAAATCACCAAAGAAGCCCCTAACTACCGCGTAGGCTGAGGCTTCCAGCTACAACATAAAGCgg                <  2:74370/150‑1 (MQ=255)                                                                                                                                 gcAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGCGCCGCCATGGACGAGTCCCTCCTGTTCGATGTGCATCTCTTCGACGCATTCCGTATCTTCCGCGTAGGCTGAGGCTTCCAGCTACAACATAAAGCgggg              <  2:971444/150‑1 (MQ=255)                                                                                                                                 atgccggcaccggtgATGCGCACGAACTCCGGCTTGGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCACGTGCACGACGTGCACATCACCAAAGAAGCCCCTAACTACCGCGTAGGCTGAGGCTTCCAGCTACAACATAAAGCgggg              <  1:603361‑M1/135‑1 (MQ=255)                                                                                                                                   aaCCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCa                                                                                             <  1:209998/69‑1 (MQ=255)                                                                                                                                   aaCCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCa                                                                                             >  2:209998/1‑69 (MQ=255)                                                                                                                                   aaCCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCACGTGCACGACGTGCAAATCACCAAAGAAGCCCCTAACTACCGCGTAGGCTGAGGCTTCCAGCTACAACATAAAGCGGGGCt            <  1:257449/150‑1 (MQ=255)                                                                                                                                   aaCCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCACGTGCACGACGTGCAAATCACCAAAGAAGCCCCTAACTACCGCGTAGGCTGAGGCTTCCAGCTACAACATAAAGCGGGGCt            >  1:52891/1‑150 (MQ=255)                                                                                                                                        tCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCACGTGCACGACGTGCAAATCACCAAAGAAGCCCCTAACTACCGCGTAGGCTGAGGCTTCCAGCTACAACATAAAGCGGGGCTGTCAt       <  2:747354/150‑1 (MQ=255)                                                                                                                                        tCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCACGTGCACGACGTGCAAATCACCAAAGAAGCCCCTAACTACCGCGTAGGCTGAGGCTTCCAGCTACAACATAAAGCGGGGCTGTCAt       <  2:175508/150‑1 (MQ=255)                                                                                                                                           aagaaACGCGCACCAAGCAGGAGCTCGTGCGCATCACCGGTACAGGCATGGCCGAGTCCCACGTGCACGACGTGCAAATCACCAAAGAAGCCCCTAACTACCGCGTAGGCTGAGGCTTCCAGCTACAACATAAAGCGGGGCTGTCATGAc    <  2:336208/150‑1 (MQ=255)                                                                                                                                             gaaATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCACGTGCACGACGTGCAAATCACCAAAGAAGCCCCTAACTACCGCGTAGGCTGAGGCTTCCAGCTACAACATAAAGCGGGGCTGTCATGACAg  <  2:374122/150‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                   CCTCGGCCGGCGCCGAGAAGCTGGTACCGGAAGGCATCGAAGGCCGCGTTCCTTACAAAGGCGCCCTGGCCGCGATCATCCACCAGCTGATGGGCGGCCTGCGTTCGTCCATGGGCTACACCGGCAGCGCAACCATCGAAGAAATGCGCACCAAGCCGGAGTTCGTGCGCATCACCGGTGCCGGCATGGCCGAGTCCCACGTGCACGACGTGCAAATCACCAAAGAAGCCCCTAACTACCGCGTAGGCTGAGGCTTCCAGCTACAACATAAAGCGGGGCTGTCATGACAG  >  NC_002947/1176795‑1177084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.