Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_002947	5,701,573	Δ61 bp	9.8%	intergenic (+20/‑132)	pyrC' → / → hslV	dihydroorotase‑like protein/ATP‑dependent HslVU protease peptidase subunit

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	= 5701572	248 (0.780)	18 (0.060)	9/256	NT	9.8%	intergenic (+19/‑193)	pyrC'/hslV	dihydroorotase‑like protein/ATP‑dependent HslVU protease peptidase subunit
	?	NC_002947	5701634 =	97 (0.320)					intergenic (+81/‑131)	pyrC'/hslV	dihydroorotase‑like protein/ATP‑dependent HslVU protease peptidase subunit

CTGTTCGACCCGCAAGCGTCCACCGTTGCTGGCGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947/5701416‑5701572 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑agcgggcaTGCCCGCGAATGCCTTCACATAGTCCCCCCGCCAAGCGCGACCATTCATCCCCCCCGGTTGAAATCCTTCCCCCCACCCCCATATGAGTCTGCATCAGGCACTTTCGCCCCGCTGCGTGGAGACTCTCCCCTTGACCACAATCGTTTCT  >  NC_002947/5701634‑5701782                                                                                                                                                                                                                                                                                                                    CTGTTCGACCCGCAAGCGTCCACCGTTGCTGGCGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGTCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTA                                                                                                                                                              <  1:6968384/150‑1 CTGTTCGACCCGCAAGCGTCCACCGTTGCTGGCGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTA                                                                                                                                                              <  2:5129107/150‑1 CTGTTCGACCCGCAAGCGTCCACCGTTGCTGGCGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTA                                                                                                                                                              <  2:5128689/150‑1 CTGTTCGACCCGCAAGCGTCCACCGTTGCTGGCGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTA                                                                                                                                                              <  1:3054720/150‑1  TGTTCGACCCGCAAGCGTCCACCGTTGCTGGCGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCCTGCTTAG                                                                                                                                                             >  2:3159188/1‑150  TGTTCGACCCGCAAGCGTCCACCGTTGCTGGCGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAG                                                                                                                                                             >  2:1641428/1‑150   GTTCGACCCGCAAGCGTCCACCGTTGCTGGCGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGC                                                                                                                                                            <  2:5411789/150‑1   GTTCGACCCGCAAGCGTCCACCGTTGCTGGCGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGC                                                                                                                                                            >  2:405034/1‑150   GTTCGACCCGCAAGCGTCCACCGTTGCTGGCGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGC                                                                                                                                                            <  2:194719/150‑1    TTCGACCCGCAAGCGTCCACCGTTGCTGGCGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCG                                                                                                                                                           <  2:604781/150‑1    TTCGACCCGCAAGCGTCCACCGTTGCTGGCGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCG                                                                                                                                                           <  2:4320573/150‑1    TTCGACCCGCAAGCGTCCACCGGTGCTGGCGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCCCATCTGCCACGAGGCCGGAGTACCAGGCTTAGCG                                                                                                                                                           >  2:7428508/1‑150     TCGACCCGCAAGCGTCCACCGTTGCTGGCGAACTATGTTTCTCCCGTGGCGAGATCTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTTGGTTATTCCTTGGTCGATGGCCACCTCTGGCACGAGGCCTGAGTACCATTCTTAGCGG                                                                                                                                                          <  1:1291170/150‑1      CGACCCGCAAGCGTCCACCGTTGCTGGCGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGG                                                                                                                                                         <  1:6114038/150‑1      CGACCCGCAAGCGTCCACCGTTGCTGGCGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGG                                                                                                                                                         <  2:3022450/150‑1      CGACCCGCAAGCGTCCACCGTTGCTGGCGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGG                                                                                                                                                         >  1:6510185/1‑150      CGACCCGCAAGCGTCCACCGTTGCTGGCGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGG                                                                                                                                                         >  1:6510397/1‑150                  GTCCACCGTTGCTGGCGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGGCGGGCGCGGTGCGCTAGCCCTCGGTCGATGGCCACATCTGCCACGATTCCTGAGTCCCATGGGTAGCGGGCATGCCCGCGAA                                                                                                                                             <  2:2451078/150‑1                  GTCCACCGTTGCTGGCGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCGTGCCCGCTAA                                                                                                                                             <  2:4852036/150‑1                  GTCCACCGTTGCTGGCGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCGTGCCCGCTAA                                                                                                                                             <  1:509443/150‑1                  GTCCACCGTTGCTGGCGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCGTGCCCGCTAA                                                                                                                                             <  1:7430832/150‑1                  GTCCACCGTTGCTGGCGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCGTGCCCGCTAA                                                                                                                                             <  1:6121781/150‑1                  GTCCACCGTTGCTGGCGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCGTGCCCGCTAA                                                                                                                                             <  2:650090/150‑1                   TCCACCGTTGCTGGCGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCGTGCCCGCTAAg                                                                                                                                            <  1:752456/150‑2                       CCGTTGCTGGCGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCATGCCCGCGAATGCCT                                                                                                                                        <  2:7431354/150‑1                                                  GTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCGTGCCCGCTAA                                                                                                                                             >  1:2692763/1‑118                                                  GTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCGTGCCCGCTAA                                                                                                                                             <  2:2692763/118‑1                                                     GCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCAAGAGGCCTGAGTACCATGCTTAGCGGGCGTGCCCGCTAA                                                                                                                                             <  1:2158598/115‑1                                                     GCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCAAGAGGCCTGAGTACCATGCTTAGCGGGCGTGCCCGCTAA                                                                                                                                             >  2:2158598/1‑115                                                                               ttCCTGGCGGTCGCGGTTCGTTATACCGTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCATGCCCGCGAATGCCTTCACATAGTCCCCCCGCCAAGCGCGACCATTCATCCCCCCCGGTTGAAATCCTTCC                                                                                <  1:3039643/148‑1                                                                                            gGGTTCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGTCCTGTTTACCATGCTTAGCGGGCATGCCCGCGAATGCCTTCACATAGTCCCCCCGCCAAGCGCGACCATTCATCCCCCCCGGTTGAAATCCTTCCCCCCACCCCCATA                                                                   <  1:2423336/149‑1                                                                                                       ACCTTTGTCGATGTCCACATTTGCAAGGATGGCTGTTTACCATTGTTAGCGGGCATGCCCGCGAATGCCTTCACATAGTCCCCCCGCCAAGCGCGACCATTCATCCCCCCCGGTTGAAATCCTTCCCCCCACCCCCATATGAGTCTGCAT                                                        <  1:6739738/150‑1                                                                                                          ggtGTCGATGGCCACATGTGCCACGAGGCTTGTTTCCCATGCTTAGCGGGCATGCCCGCGAATGCCTTCACATAGTCCCCCCGCCAAGCGCGACCATTCATCCCCCCCGGTTGAAATCCTTCCCCCCACCCCCATATGAGTCTGCATCAG                                                     <  1:7428508/147‑1                                                                                                           ccGTCGATCGCCCCATGTGCCAGGCGGCCTGAGTCCCATGCTTAGCGGGCATGCCCGCGAATGCCTTCACATAGTCCCCCCGCCAATCGCGACCTTTCATCCCCCCCGGTTGAAATCCTTCCCCCCACCCCCATATGAGTCTGCATCCGG                                                    <  2:4324421/148‑1                                                                                                           TGGTCGATGGCCACATCTGCCACGAGGCCTGATTACCATGGTTAGCGGGCATGCCCGCGAATGCCTTCACATAGTCCCCCCGCCAAGCGCGACCATTCATCCCCCCCGGTTGAAATCCTTCCCCCCACCCCCATATGAGTCTGCATCAGG                                                    <  1:2988285/150‑1                                                                                                                                               catgggTAGCGGGCATGCCCGCGAATGCCTTCACATAGTCCCCCCGCCAAGCGCGACCATTCATCCCCCCCGGTTGAAATCCTTCCCCCCACCCCCATATGAGTCTGCATCAGGCACTTTCGCCCCGCTGCGTGGAGACTCTCCCCTTGA                <  1:1733517/144‑1                                                                                                                                                      AGCGGGCATGCCCGCGAATGCCTTCACATAGTCCCCCCGCCAAGCGCGACCATTCATCCCCCCCGGTTGAAATCCTTCCCCCCACCCCCATATGAGTCTGCATCAGGCACTTTCGCCCCGCTGCGTGGAGACTCTCCCCTTGACCACAAT         <  1:6991102/150‑1                                                                                                                                                       GCGGGCATGCCCGCGAATGCCTTCACATAGTCCCCCCGCCAAGCGCGACCATTCATCCCCCCCGGTTGAAATCCTTCCCCCCACCCCCATATGAGTCTGCATCAGGCACTTTCGCCCCGCTGCGTGGAGACTCTCCCCTTGACCACAATC        <  1:597385/150‑1                                                                                                                                                          GGCATGCCCGCGAATGCCTTCACATAGTCCCCCCGCCAAGCGCGACCATTCATCCCCCCCGGTTGAAATCCTTCCCCCCACCCCCATATGAGTCTGCATCAGGCACTTTCGCCCCGCTGCGTGGAGACTCTCCCCTTGACCACAATCGTT     <  1:4629542/150‑1                                                                                                                                                          GGCATGCCCGCGAATGCCTTCACATAGTCCCCCCGCCAAGCGCGACCATTCATCCCCCCCGGTTGAAATCCTTCCCCCCACCCCCATATGAGTCTGCATCAGGCACTTTCGCCCCGCTGCGTGGAGACTCTCCCCTTGACCACAATCGTT     <  1:5400600/150‑1                                                                                                                                                           GCATGCCCGCGAATGCCTTCACATAGTCCCCCCGCCAAGCGCGACCATTCATCCCCCCCGGTTGAAATCCTTCCCCCCACCCCCATATGAGTCTGCATCAGGCACTTTCGCCCCGCTGCGTGGAGACTCTCCCCTTGACCACAATCGTTT    <  1:2565594/150‑1                                                                                                                                                           GCATGCCCGCGAATGCCTTCACATAGTCCCCCCGCCAAGCGCGACCATTCATCCCCCCCGGTTGAAATCCTTCCCCCCACCCCCATATGAGTCTGCATCAGGCACTTTCGCCCCGCTGCGTGGAGACTCTCCCCTTGACCACAATCGTTT    <  1:6049808/150‑1                                                                                                                                                             ATGCCCGCGAATGCCTTCACATAGTCCCCCCGCCAAGCGCGACCATTCATCCCCCCCGGTTGAAATCCTTCCCCCCACCCCCATATGAGTCTGCATCAGGCACTTTCGCCCCGCTGCGTGGAGACTCTCCCCTTGACCACAATCGTTTCT  <  1:3196765/150‑1                                                                                                                                                             ATGCCCGCGAATGCCTTCACATAGTCCCCCCGCCAAGCGCGACCATTCATCCCCCCCGGTTGAAATCCTTCCCCCCACCCCCATATGAGTCTGCATCAGGCACTTTCGCCCCGCTGCGTGGAGACTCTCCCCTTGACCACAATCGTTTCT  <  1:6738705/150‑1                                                                                                                                                             ATGCCCGCGAATGCCTTCACATAGTCCCCCCGCCAAGCGCGACCATTCATCCCCCCCGGTTGAAATCCTTCCCCCCACCCCCATATGAGTCTGCATCAGGCACTTTCGCCCCGCTGCGTGGAGACTCTCCCCTTGACCACAATCGTTTCT  <  1:1614698/150‑1                                                                                                                                                             ATGCCCGCGAATGCCTTCACATAGTCCCCCCGCCAAGCGCGACCATTCATCCCCCCCGGTTGAAATCCTTCCCCCCACCCCCATATGAGTCTGCATCAGGCACTTTCGCCCCGCTGCGTGGAGACTCTCCCCTTGACCACAATCGTTTCT  <  1:1196009/150‑1                                                                                                                                                                                                                                                                                                                    CTGTTCGACCCGCAAGCGTCCACCGTTGCTGGCGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947/5701416‑5701572 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑agcgggcaTGCCCGCGAATGCCTTCACATAGTCCCCCCGCCAAGCGCGACCATTCATCCCCCCCGGTTGAAATCCTTCCCCCCACCCCCATATGAGTCTGCATCAGGCACTTTCGCCCCGCTGCGTGGAGACTCTCCCCTTGACCACAATCGTTTCT  >  NC_002947/5701634‑5701782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.