breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsJBEI_Xylose_A28_F48_I1_S30_R1_001.good.fq1,168,642155,403,803100.0%133.0 bases136 bases96.8%
errorsJBEI_Xylose_A28_F48_I1_S30_R2_001.good.fq1,168,642155,403,803100.0%133.0 bases136 bases82.9%
total2,337,284310,807,606100.0%133.0 bases136 bases89.8%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionNC_0029476,183,85843.78.1100.0%na
total6,183,858100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 1000004410
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 2
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 5000215
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.010

Junction Skew Score Calculation

reference sequencepr(no read start)
NC_0029470.89052

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 3
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 1

Read Alignment Evidence

optionvalue
ModeConsensus/Mixed Base
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff10
Polymorphism frequency cutoff0.2
Polymorphism minimum variant coverage each strandOFF
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs ofOFF
Skip base substitutions when they create a homopolymer flanked on each side byOFF

Software Versions

programversion
bowtie22.3.4.1
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input00:06:27 18 Jul 202000:07:27 18 Jul 20201 minute 0 seconds
Read alignment to reference genome00:07:28 18 Jul 202000:12:45 18 Jul 20205 minutes 17 seconds
Preprocessing alignments for candidate junction identification00:12:45 18 Jul 202000:13:47 18 Jul 20201 minute 2 seconds
Preliminary analysis of coverage distribution00:13:47 18 Jul 202000:14:59 18 Jul 20201 minute 12 seconds
Identifying junction candidates00:14:59 18 Jul 202000:15:01 18 Jul 20202 seconds
Re-alignment to junction candidates00:15:01 18 Jul 202000:16:19 18 Jul 20201 minute 18 seconds
Resolving best read alignments00:16:19 18 Jul 202000:18:02 18 Jul 20201 minute 43 seconds
Creating BAM files00:18:02 18 Jul 202000:19:05 18 Jul 20201 minute 3 seconds
Tabulating error counts00:19:05 18 Jul 202000:19:47 18 Jul 202042 seconds
Re-calibrating base error rates00:19:47 18 Jul 202000:19:49 18 Jul 20202 seconds
Examining read alignment evidence00:19:49 18 Jul 202000:26:51 18 Jul 20207 minutes 2 seconds
Polymorphism statistics00:26:51 18 Jul 202000:26:52 18 Jul 20201 second
Output00:26:52 18 Jul 202000:27:35 18 Jul 202043 seconds
Total 21 minutes 7 seconds